Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,977	A→G	M397V (ATG→GTG)	yagH →	CP4‑6 prophage, putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,977	0	A	G	100.0%	91.7 / NA	26	M397V (ATG→GTG)	yagH	CP4‑6 prophage, putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)

GACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAG  >  NC_000913/287845‑288078                                                                                                                                     |                                                                                                      gacACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGt                                                                                                 >  1:1008622/1‑139 (MQ=255)            ctcgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTg                                                                                      <  2:1008622/139‑1 (MQ=255)             tcgctcACCGCGCGCCCGCGCTTCATCCTACTCTATGGCAACGACTCGCTTTATCCCACCTTCACCCCATCGACGGTAGTGGGCCGCTGGGAGCACATAACCTTCCTGGCAAAAACGCGGGTGTAGCTCTGGACGGTGc                                                                                     >  1:739618/1‑139 (MQ=255)             ccgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                       >  2:516444/2‑137 (MQ=255)             ccgctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGt                                                                                       <  1:516444/136‑1 (MQ=255)               gctcACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCAc                                                                                   <  1:715326/139‑1 (MQ=255)                         cgcCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCaga                                                                         >  2:1058555/1‑139 (MQ=255)                                     ttACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGa                                                             <  2:383584/139‑1 (MQ=255)                                       aCGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAcc                                                           <  1:674697/139‑1 (MQ=255)                                        cGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCt                                                          <  1:253029/139‑1 (MQ=255)                                          gCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGc                                                        >  1:169447/1‑139 (MQ=255)                                           ctctATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGct                                                       <  1:214393/139‑1 (MQ=255)                                                   cAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTacaac                                               <  2:169447/139‑1 (MQ=255)                                                        aCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                            <  1:364351/121‑1 (MQ=255)                                                        aCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGAc                                                            >  2:364351/1‑121 (MQ=255)                                                        aCTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCaa                                          >  1:108870/1‑139 (MQ=255)                                                          tCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTg                                                                                                     >  2:925854/1‑78 (MQ=255)                                                          tCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTg                                                                                                     >  2:168844/1‑78 (MQ=255)                                                                          tCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTg                                                                                                     >  2:997154/1‑62 (MQ=255)                                                                                             gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa     <  1:1058555/139‑1 (MQ=255)                                                                                             gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa     <  2:737591/139‑1 (MQ=255)                                                                                             gcgcCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGa     <  2:108870/139‑1 (MQ=255)                                                                                                ccgcTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAg  >  1:985512/1‑139 (MQ=255)                                                                                                                gCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                      <  2:184239/55‑1 (MQ=255)                                                                                                                gCCTTCCGGGCAGAAACGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAgcg                                                                      >  1:184239/1‑55 (MQ=255)                                                                                                                             aaaCGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGAc                 <  2:1097445/95‑1 (MQ=255)                                                                                                                             aaaCGCGGGTGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGAc                 >  1:1097445/1‑95 (MQ=255)                                                                                                                                     |                                                                                                      GACACCCTCGGCTCGCTCACCGCGCGCCCGGGCTTCTTACGGCTCTATGGCAACGACTCGCTCAATTCGACCTTCACCCAATCGACCGTGGCGCGCCGCTGGCAGCACTTCGCCTTCCGGGCAGAAACGCGGATGGAGTTCTCGCCGGTGCACTTCCAGCAGAGCGCGGGGCTGACCTGCTACTACAACAGCAAAAACTGGAGCTACTGCTTTGTGGACTACGAGGAGGGACAG  >  NC_000913/287845‑288078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑