Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,173	C→T	*212* (TAG→TAA)	argO ←	arginine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,173	0	C	T	100.0%	143.5 / NA	45	*212* (TAG→TAA)	argO	arginine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/26);  total (19/26)

CATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGC  >  NC_000913/3068045‑3068285                                                                                                                                 |                                                                                                                 cATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACa                                                                                                        <  2:470310/139‑1 (MQ=255)  aTTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACaa                                                                                                       >  1:380984/1‑139 (MQ=255)      aTGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCt                                                                                                   <  1:276951/139‑1 (MQ=255)       tGCCGCCAGGGCGATAACTTTAAACTTAACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTAAGGACATCTGGAGGCAAGCTTAGCGCCTCAGTTTTATTTTTCCATCAGATACCGCTTAACTGCACAAGGcat                                                                                                  >  1:1080943/1‑137 (MQ=255)         ccgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACa                                                                                                        <  1:654201/131‑1 (MQ=255)         ccgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACa                                                                                                        >  2:654201/1‑131 (MQ=255)          cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTtgtg                                                                                               >  1:943475/1‑139 (MQ=255)          cgccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTtgtg                                                                                               >  1:75087/1‑139 (MQ=255)           gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                              <  2:361552/139‑1 (MQ=255)           gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                              <  1:337944/139‑1 (MQ=255)           gccAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                              <  2:75087/139‑1 (MQ=255)                      aaCTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATAc                                                                                   <  1:1145660/139‑1 (MQ=255)                                    cGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGc                                                                     <  1:746363/139‑1 (MQ=255)                                       tAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGc                                                                  >  1:187919/1‑139 (MQ=255)                                        aGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCa                                                                 <  2:753667/139‑1 (MQ=255)                                           ctctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAgg                                                              <  1:388497/139‑1 (MQ=255)                                             ctcCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCa                                                            >  2:877835/1‑139 (MQ=255)                                                  tgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                       <  2:187919/139‑1 (MQ=255)                                                  tgtTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATaaa                                                       <  1:877835/139‑1 (MQ=255)                                                      aaTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                        <  2:969705/118‑1 (MQ=255)                                                      aaTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                        >  1:969705/1‑118 (MQ=255)                                                       aTTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGCTAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACcaca                                                  <  1:698536/139‑1 (MQ=255)                                                        ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCtt                                                                                                  <  1:136758/90‑1 (MQ=255)                                                        ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCtt                                                                                                  >  2:136758/1‑90 (MQ=255)                                                        ttGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACAt                                                 >  2:532419/1‑139 (MQ=255)                                                          gACTTCCCCAAAATGCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATaa                                               <  1:615447/139‑1 (MQ=255)                                                            cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTa                                                                                                                <  1:227182/72‑1 (MQ=255)                                                            cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                              <  2:770049/90‑1 (MQ=255)                                                            cTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGc                                                                                              >  1:770049/1‑90 (MQ=255)                                                                cccAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATc                                         >  2:90786/1‑139 (MQ=255)                                                                   aaaaTTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTA‑‑TTTCCATCAGATAGCGCTTAACTGGACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACGACATAACACATCCcaca                                    <  1:697935/139‑1 (MQ=255)                                                                        tCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAAcc                                 <  1:75686/139‑1 (MQ=255)                                                                          aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGc                                                                                                    <  2:650636/70‑1 (MQ=255)                                                                          aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGc                                                                                                    >  1:650636/1‑70 (MQ=255)                                                                          aGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAg                               >  1:826199/1‑139 (MQ=255)                                                                                            cTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                               >  1:655184/1‑41 (MQ=255)                                                                                            cTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCTTaa                                                                                                               <  2:655184/41‑1 (MQ=255)                                                                                                   ccTCTGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGcc      <  2:826199/139‑1 (MQ=255)                                                                                                      ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                        <  2:83230/70‑1 (MQ=255)                                                                                                      ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGcc                                                                        >  1:83230/1‑70 (MQ=255)                                                                                                      ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                            >  2:172256/1‑98 (MQ=255)                                                                                                      ctGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAacac                                            <  1:172256/98‑1 (MQ=255)                                                                                                       tGTTTTATTTTTCCATCAGATAGCGCTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTgc  >  2:196571/1‑139 (MQ=255)                                                                                                                              cgcTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                         <  2:1122330/61‑1 (MQ=255)                                                                                                                              cgcTTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATa                                                         >  1:1122330/1‑61 (MQ=255)                                                                                                                                 |                                                                                                                 CATTAATGCCGCCAGGGCGATAACTTTGAACTTCACGTTAGTCTCTCCGTGTTAATTGACTTGCCCAAAATTCAGGACCTCTGGAGGCAAGCTTAGCGCCTCTGTTTTATTTTTCCATCAGATAGCGCCTAACTGAACAAGGCTTGTGCATGAGCAATACCGTCTCTCGCCAGCTGCAAGGCAATAAACCACATAACACATCCCACAACCAGATTGATAATGCGCTGTGCTTTTGCCGTGC  >  NC_000913/3068045‑3068285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑