Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,131,341	C→T	*356* (TAG→TAA)	yghQ ←	putative inner membrane polysaccharide flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,131,341	0	C	T	100.0%	133.2 / NA	42	*356* (TAG→TAA)	yghQ	putative inner membrane polysaccharide flippase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/24);  total (18/24)

TAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAAC  >  NC_000913/3131213‑3131476                                                                                                                                 |                                                                                                                                        tAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGt                                                                                                                               >  1:177877/1‑139 (MQ=18)     tGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATa                                                                                                                           <  2:177877/139‑1 (MQ=25)       gCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACg                                                                                                                         <  1:176062/139‑1 (MQ=38)         gaagaaACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       <  2:989398/139‑1 (MQ=255)         gaagaaACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       >  2:396758/1‑139 (MQ=255)            gaaACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCg                                                                                                                    <  1:936291/139‑1 (MQ=255)                   tCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACt                                                                                                             <  1:614985/139‑1 (MQ=255)                     tAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgaga                                                                                                                 >  1:108422/1‑133 (MQ=255)                     tAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgaga                                                                                                                 <  2:108422/133‑1 (MQ=255)                       aaTAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       <  1:1072027/125‑1 (MQ=255)                       aaTAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       >  2:1072027/1‑125 (MQ=255)                        aTAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACg                                                                                                        <  2:545362/139‑1 (MQ=255)                            gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgaga                                                                                                                 <  1:611994/126‑1 (MQ=255)                            gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgaga                                                                                                                 >  2:611994/1‑126 (MQ=255)                             cTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCaaa                                                                                                   <  1:656840/139‑1 (MQ=255)                             cTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCaaa                                                                                                   >  2:81938/1‑139 (MQ=255)                                 ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTaa                                                                                                          <  2:941105/128‑1 (MQ=255)                                 ttCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTaa                                                                                                          >  1:941105/1‑128 (MQ=255)                                    aaGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGc                                                                                            <  1:1189668/139‑1 (MQ=255)                                           ttACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGa                                                                                     <  1:81938/139‑1 (MQ=255)                                           ttACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGa                                                                                     >  2:65944/1‑139 (MQ=255)                                            tACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAg                                                                                    <  1:65944/139‑1 (MQ=255)                                                  agaAAAAATCGGCCCACACCTGAAGAAATGGAATGTCTTAGTCCCCTGAGAAGAATTTCCCAACTTATTCGCCCCTTCCTTAAGCCAGAAACTACGCCTCGAGATACTTAACGCCAAACACCAGCGAAACGAGCGGCtc                                                                              >  1:303367/1‑138 (MQ=255)                                                    aaaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCa                                                                                                     <  1:406475/114‑1 (MQ=255)                                                    aaaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCa                                                                                                     >  2:406475/1‑114 (MQ=255)                                                                        aagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAgc                                                        <  2:79604/139‑1 (MQ=255)                                                                           aaatgaaatgACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGcc                                                     >  1:3373/1‑139 (MQ=255)                                                                                        gAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGa                                        >  1:459198/1‑139 (MQ=255)                                                                                             aGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccg                                   >  2:1108984/1‑139 (MQ=255)                                                                                               ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccgcc                                 <  2:3373/139‑1 (MQ=255)                                                                                                cGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTccgccc                                >  2:783884/1‑139 (MQ=255)                                                                                                           tCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAAcc                     <  1:396758/139‑1 (MQ=255)                                                                                                                   tATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTca             <  2:459198/139‑1 (MQ=255)                                                                                                                     ttCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTcaca           <  2:392315/139‑1 (MQ=255)                                                                                                                        gCACCTTCCTTAAATCAGGTCATACGCGTCGAGATACTTAACGCCAATCCCCCGGGAAATGCGCTGCTTGCCGACGATCAACCCCGCGAGGGCCACCAGAACACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCt        <  2:303367/139‑1 (MQ=255)                                                                                                                          aCCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTa                                                                                                           >  1:960933/1‑38 (MQ=255)                                                                                                                          aCCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTa                                                                                                           <  2:960933/38‑1 (MQ=255)                                                                                                                            cTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTcaca           <  2:761888/132‑1 (MQ=255)                                                                                                                            cTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTcaca           >  1:761888/1‑132 (MQ=255)                                                                                                                              tCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTcac            <  2:753510/129‑1 (MQ=255)                                                                                                                              tCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTcac            >  1:753510/1‑129 (MQ=255)                                                                                                                              tCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTaacaac  >  1:1192110/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                        TAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAAC  >  NC_000913/3131213‑3131476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑