Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,176,858	C→T	*141* (TAG→TAA)	yqiB ←	DUF1249 protein YqiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,176,858	0	C	T	100.0%	163.3 / NA	51	*141* (TAG→TAA)	yqiB	DUF1249 protein YqiB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/24);  total (27/24)

TTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAG  >  NC_000913/3176725‑3176990                                                                                                                                      |                                                                                                                                     ttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTtaata                                                                                                                                 >  1:6938/1‑139 (MQ=255)          aCAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAAt                                                                                                                        >  1:441329/1‑139 (MQ=255)             gggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGc                                                                                                                     <  2:6938/139‑1 (MQ=255)                 ttCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATc                                                                                                                 <  1:1016361/139‑1 (MQ=255)                   cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGc                                                                                                               >  2:938925/1‑139 (MQ=255)                      gCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                                            <  1:800559/139‑1 (MQ=255)                           tgtgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                       <  1:264152/139‑1 (MQ=255)                           tgtgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                       <  1:762162/139‑1 (MQ=255)                           tgtgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                       <  2:441329/139‑1 (MQ=255)                                           tgtCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCaa                                                                                       >  2:489184/1‑139 (MQ=255)                                              cGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                                            <  2:817939/115‑1 (MQ=255)                                              cGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                                            >  1:817939/1‑115 (MQ=255)                                                       gTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaa                                                                           >  2:1164839/1‑139 (MQ=255)                                                          aaaTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaaaaa                                                                        <  1:489184/139‑1 (MQ=255)                                                             tCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                                            >  2:907762/1‑100 (MQ=255)                                                             tCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                                            <  1:907762/100‑1 (MQ=255)                                                                                agccagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTtcg                                                  <  1:938925/139‑1 (MQ=255)                                                                                  ccagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                                            <  2:781333/79‑1 (MQ=255)                                                                                  ccagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTcc                                                                                                            >  1:781333/1‑79 (MQ=255)                                                                                      agGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCa                                                                                                                   >  2:1090114/1‑68 (MQ=255)                                                                                      agGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCa                                                                                                                   <  1:1090114/68‑1 (MQ=255)                                                                                           gggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATg                                       >  2:162018/1‑139 (MQ=255)                                                                                            ggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGc                                      >  2:1106380/1‑139 (MQ=255)                                                                                             gTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCa                                     >  2:441951/1‑139 (MQ=255)                                                                                                aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTcgc                                              <  1:971754/127‑1 (MQ=255)                                                                                                aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTcgc                                              >  2:971754/1‑127 (MQ=255)                                                                                                aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATg                                       >  2:1047166/1‑134 (MQ=255)                                                                                                aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATg                                       <  1:1047166/134‑1 (MQ=255)                                                                                                     gCTTTCCAAATGGTGTCCTTAGGTTTCACGACATTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTtta                             <  1:924860/139‑1 (MQ=255)                                                                                                       tttCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCg                                                                                                                <  2:1140146/54‑1 (MQ=255)                                                                                                       tttCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCg                                                                                                                >  1:1140146/1‑54 (MQ=255)                                                                                                       tttCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                           <  1:457433/139‑1 (MQ=255)                                                                                                        ttCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatta                          >  2:557098/1‑139 (MQ=255)                                                                                                           cAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                       <  1:557098/139‑1 (MQ=255)                                                                                                                 gtgtCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCa                 >  2:79878/1‑139 (MQ=255)                                                                                                                   gtCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAt                                                               <  1:638905/91‑1 (MQ=255)                                                                                                                   gtCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAAt                                                               >  2:638905/1‑91 (MQ=255)                                                                                                                   gtCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATa               >  2:79796/1‑139 (MQ=255)                                                                                                                   gtCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATa               >  1:962972/1‑139 (MQ=255)                                                                                                                   gtCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATa               >  2:643519/1‑139 (MQ=255)                                                                                                                    tCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAGCCAGt                                                                                  <  2:538988/71‑1 (MQ=255)                                                                                                                    tCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                  >  1:538988/1‑71 (MQ=255)                                                                                                                    tCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                         <  1:854634/128‑1 (MQ=255)                                                                                                                    tCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                         >  2:854634/1‑128 (MQ=255)                                                                                                                     ccTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                       >  2:891280/1‑49 (MQ=255)                                                                                                                     ccTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                       <  1:891280/49‑1 (MQ=255)                                                                                                                            tttCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGc                                      >  2:810913/1‑107 (MQ=255)                                                                                                                            tttCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGc                                      <  1:810913/107‑1 (MQ=255)                                                                                                                              tCACGACGTTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                       <  1:461298/40‑1 (MQ=255)                                                                                                                              tCACGACGTTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                       >  2:461298/1‑40 (MQ=255)                                                                                                                                acgacgTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCTTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAg  >  2:88101/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                     TTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAG  >  NC_000913/3176725‑3176990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑