Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,191,696	G→A	*355* (TAG→TAA)	yqiI →	fimbrial protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,191,696	0	G	A	100.0%	126.5 / NA	40	*355* (TAG→TAA)	yqiI	fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/20);  total (20/20)

ATTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAA  >  NC_000913/3191561‑3191806                                                                                                                                        |                                                                                                               aTTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAatat                                                                                                             <  1:1159225/139‑1 (MQ=255) aTTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAatat                                                                                                             <  2:739839/139‑1 (MQ=255) aTTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAatat                                                                                                             <  2:594385/139‑1 (MQ=255)              tCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAacac                                                                                                <  1:676821/139‑1 (MQ=255)                              cTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATAc                                                                                                      >  1:724558/1‑117 (MQ=255)                              cTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGCTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATAc                                                                                                      <  2:724558/117‑1 (MQ=255)                                 cGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGcc                                                                             <  2:1001874/139‑1 (MQ=255)                                          cACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATc                                                                    <  2:514730/139‑1 (MQ=255)                                              aTTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCa                                                                   <  2:200793/136‑1 (MQ=255)                                              aTTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCa                                                                   >  1:200793/1‑136 (MQ=255)                                               ttCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGc                                                               >  1:1050438/1‑139 (MQ=255)                                                tCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGct                                                              <  2:1050438/139‑1 (MQ=255)                                                    ggCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAGATCACCTATAACTAATATCTAATACa                                                                                                     >  1:465894/1‑96 (MQ=255)                                                    ggCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                     <  2:465894/96‑1 (MQ=255)                                                       aGAACTGGGTCCGAACCCGGCAGAAACGGGTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCCCCTCTAACTAATATCTAATACAAACACTAAAACGGGCGATCAGGCCCGTTATATCAGTGCTCTAACTc                                                       <  1:553401/139‑1 (MQ=255)                                                         aaCTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCa                                                     >  2:340601/1‑139 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAg                                                                  >  1:164993/1‑121 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAg                                                                  >  1:135509/1‑121 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAg                                                                  <  2:135509/121‑1 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAg                                                                  <  2:164993/121‑1 (MQ=255)                                                              aGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGctct                                                >  2:630987/1‑139 (MQ=255)                                                                  cGAACCCGGCAGAAACGGTTATTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAgg                                                                               >  1:633543/1‑104 (MQ=255)                                                                  cGAACCCGGCAGAAACGGTTATTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAgg                                                                               <  2:633543/104‑1 (MQ=255)                                                                     aCCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACgg                                                                                        <  2:1049856/92‑1 (MQ=255)                                                                     aCCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACgg                                                                                        >  1:1049856/1‑92 (MQ=255)                                                                     aCCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCt                                         <  1:630987/139‑1 (MQ=255)                                                                                          aGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAcc                    >  1:905699/1‑139 (MQ=255)                                                                                              aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                     <  2:1102248/54‑1 (MQ=255)                                                                                              aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACa                                                                                                     >  1:1102248/1‑54 (MQ=255)                                                                                              aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTc                                                       >  1:46677/1‑100 (MQ=255)                                                                                              aCCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTc                                                       <  2:46677/100‑1 (MQ=255)                                                                                                    tAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACg          <  2:905699/139‑1 (MQ=255)                                                                                                    tAGTAAAGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACg          >  1:693345/1‑139 (MQ=255)                                                                                                         aaGACGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTgcgc     >  2:1150542/1‑139 (MQ=255)                                                                                                            aCGTAATCCTGAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCaaa  >  2:417569/1‑139 (MQ=255)                                                                                                                      gAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAgg                                                                               >  2:13482/1‑52 (MQ=255)                                                                                                                      gAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAgg                                                                               <  1:13482/52‑1 (MQ=255)                                                                                                                      gAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGtt                             >  2:280712/1‑102 (MQ=255)                                                                                                                      gAAAATCACCTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGtt                             <  1:280712/102‑1 (MQ=255)                                                                                                                              ccTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                     >  2:304296/1‑102 (MQ=255)                                                                                                                              ccTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                     >  2:1050106/1‑102 (MQ=255)                                                                                                                              ccTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                     <  1:304296/102‑1 (MQ=255)                                                                                                                              ccTATAACTAATATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATAc                     <  1:1050106/102‑1 (MQ=255)                                                                                                                                        |                                                                                                               ATTTGCTTCTTTTTCATCGTCTAGTGCGCCTTCGGTCACCGCACCATTCAGGGCAGAACTGAGTGCGAACCCGGCAGAAACGGTTGTTGAGGGACCGTTTAGTAAAGACGTAATCCTGAAAATCACCTATAACTAGTATCTAATACAAACACTAAAACGGGCCATCAGGCCCGTTATATCAGTGCTCTAACTCCAGCTCTCTTGCCTGCATCATCTGTTGACAATACCAGGCATAACGTGCGCAAA  >  NC_000913/3191561‑3191806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑