Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,277,867	C→T	V267I (GTC→ATC)	agaR ←	transcriptional repressor of the aga regulon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,867	0	C	T	100.0%	184.1 / NA	57	V267I (GTC→ATC)	agaR	transcriptional repressor of the aga regulon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/26);  total (31/26)

AATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGG  >  NC_000913/3277732‑3277998                                                                                                                                        |                                                                                                                                    aaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAg                                                                                                                                  >  1:100418/1‑139 (MQ=255) aaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAg                                                                                                                                  >  1:66436/1‑139 (MQ=255)   tGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGaa                                                                                                                                >  1:1027931/1‑139 (MQ=255)     ttAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATc                                                                                                                              <  2:1027931/139‑1 (MQ=255)        aaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACt                                                                                                                           >  1:229409/1‑139 (MQ=255)               gACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAAccc                                                                                                                    >  1:961798/1‑139 (MQ=255)                         tGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCg                                                                                                          <  2:100418/139‑1 (MQ=255)                            cGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                       <  2:66436/139‑1 (MQ=255)                              aCTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTc                                                                                                     >  1:321238/1‑139 (MQ=255)                                                 agaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                  >  2:369239/1‑139 (MQ=255)                                                 agaAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAgg                                                                                  >  1:556153/1‑139 (MQ=255)                                                     aCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                       >  2:232597/1‑114 (MQ=255)                                                     aCAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAg                                                                                                       <  1:232597/114‑1 (MQ=255)                                                       agaagaAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGcc                                                                            <  2:321238/139‑1 (MQ=255)                                                              aCCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcatca                                                                     >  2:501871/1‑139 (MQ=255)                                                                 cATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                  <  2:229409/139‑1 (MQ=255)                                                                 cATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATcaaca                                                                  >  1:714870/1‑139 (MQ=255)                                                                      aTGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCTGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                             <  1:1214124/139‑1 (MQ=255)                                                                       tGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATc                                                            >  2:167471/1‑139 (MQ=255)                                                                            ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTc                                                       >  2:131364/1‑139 (MQ=255)                                                                            ttCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTc                                                       <  2:556153/139‑1 (MQ=255)                                                                                caATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc                                                                         <  1:504389/117‑1 (MQ=255)                                                                                caATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtc                                                                         >  2:504389/1‑117 (MQ=255)                                                                                 aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTa                                                                                          >  2:886831/1‑99 (MQ=255)                                                                                 aaTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTa                                                                                          <  1:886831/99‑1 (MQ=255)                                                                                  aTGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACg                                                 <  2:714870/139‑1 (MQ=255)                                                                                    gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCTCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                    <  1:149132/134‑1 (MQ=255)                                                                                    gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGAt                                                    >  2:149132/1‑134 (MQ=255)                                                                                    gCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGtt                                               >  2:627255/1‑139 (MQ=255)                                                                                        gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                       >  2:964616/1‑111 (MQ=255)                                                                                        gccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                       <  1:964616/111‑1 (MQ=255)                                                                                         ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                     <  2:587362/128‑1 (MQ=255)                                                                                         ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGa                                                     >  1:587362/1‑128 (MQ=255)                                                                                         ccgAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTa                                          >  2:616215/1‑139 (MQ=255)                                                                                           gAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATc                                        >  2:887039/1‑139 (MQ=255)                                                                                                      tgtTGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTAt                             <  1:369239/139‑1 (MQ=255)                                                                                                        ttGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                       >  1:210896/1‑95 (MQ=255)                                                                                                        ttGACCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                       <  2:210896/95‑1 (MQ=255)                                                                                                           aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCtt                                                                          <  1:144077/89‑1 (MQ=255)                                                                                                           aCCAGCGGGCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCtt                                                                          >  2:144077/1‑89 (MQ=255)                                                                                                                 gggCATCCTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGaa                  <  1:131364/139‑1 (MQ=255)                                                                                                                        cTTTCTCACTCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGa           <  1:501871/139‑1 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                             <  2:222831/48‑1 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAgg                                                                                             >  1:222831/1‑48 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGa                                                                                 >  1:358104/1‑60 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGa                                                                                 <  2:358104/60‑1 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGAt                                  >  2:209384/1‑107 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGAt                                  <  1:209384/107‑1 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTa                          <  1:278218/115‑1 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTa                          >  2:278218/1‑115 (MQ=255)                                                                                                                                 tCCCCGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTgg  >  2:979487/1‑139 (MQ=255)                                                                                                                                  ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                       <  1:667122/69‑1 (MQ=255)                                                                                                                                  ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTtcat                                                                       >  2:667122/1‑69 (MQ=255)                                                                                                                                  ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                             >  2:362214/1‑79 (MQ=255)                                                                                                                                  ccccGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGAt                                                             <  1:362214/79‑1 (MQ=255)                                                                                                                                     cGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATg                            <  2:1186491/109‑1 (MQ=255)                                                                                                                                     cGATCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATg                            >  1:1186491/1‑109 (MQ=255)                                                                                                                                        |                                                                                                                                    AATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGGAATGCCTTCATCAACAATGATCATGTCGATACGTTGAGTATCAATGATCTTATGTAAACTGGAACGATTGAACTTACTGG  >  NC_000913/3277732‑3277998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑