Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,282,504	A→G	T177A (ACC→GCC)	agaS →	tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,282,504	0	A	G	100.0%	192.4 / NA	53	T177A (ACC→GCC)	agaS	tagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/26);  total (27/26)

AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGT  >  NC_000913/3282370‑3282635                                                                                                                                       |                                                                                                                                    aaTGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTgccac                                                                                                                                 >  2:1211145/1‑139 (MQ=255) aaTGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTgccac                                                                                                                                 >  1:383007/1‑139 (MQ=255)     cTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCatg                                                                                                                             >  1:748954/1‑139 (MQ=255)     cTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCatg                                                                                                                             >  2:128271/1‑139 (MQ=255)      tATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCatga                                                                                                                            <  2:383007/139‑1 (MQ=255)               ccGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGCTAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCt                                                                                                                   >  2:875754/1‑139 (MQ=255)               ccGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCt                                                                                                                   >  1:732635/1‑139 (MQ=255)                  aTCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCATCAGCATTGCCACCATGATGGCCAGCTGcc                                                                                                                >  1:786767/1‑139 (MQ=255)                     aCCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcg                                                                                                             <  1:128271/139‑1 (MQ=255)                      ccTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcgc                                                                                                            >  1:1011556/1‑139 (MQ=255)                         gCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                         <  2:1011556/139‑1 (MQ=255)                               aaGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGc                                                                                                   >  2:794566/1‑139 (MQ=255)                               aaGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGc                                                                                                   >  1:130668/1‑139 (MQ=255)                                    ggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              >  2:373718/1‑139 (MQ=255)                                    ggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              <  2:74281/139‑1 (MQ=255)                                             tACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     <  2:732635/139‑1 (MQ=255)                                                     cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      >  1:480686/1‑114 (MQ=255)                                                     cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      <  2:480686/114‑1 (MQ=255)                                                     cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa                                                                             >  2:888889/1‑139 (MQ=255)                                                         aTCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCt                                                                         >  1:298545/1‑139 (MQ=255)                                                          tCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      >  2:986189/1‑109 (MQ=255)                                                          tCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      <  1:986189/109‑1 (MQ=255)                                                               aGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCcgcg                                                                   <  2:130668/139‑1 (MQ=255)                                                                gCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGa                                                                  <  2:298545/139‑1 (MQ=255)                                                                gCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGa                                                                  <  1:373718/139‑1 (MQ=255)                                                                   aTAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              >  2:591791/1‑108 (MQ=255)                                                                   aTAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTg                                                                                              <  1:591791/108‑1 (MQ=255)                                                                           tttGCCCTGCTGATGCCCGCTGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGGCCCGCGGTTCTCGCACCTGAGACGATCAACAGCCAAACCTTCAGCCACGTGGCGGagc                                                       >  1:716155/1‑137 (MQ=255)                                                                               cccTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTg                                                   <  1:794566/139‑1 (MQ=255)                                                                                 ctgctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGGGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGcc                                                 <  1:1211145/139‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  2:1093112/139‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  1:19191/139‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  1:875754/139‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  1:801089/139‑1 (MQ=255)                                                                                      gATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                    >  2:78654/1‑83 (MQ=255)                                                                                      gATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                    <  1:78654/83‑1 (MQ=255)                                                                                         gCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATc                                         <  1:335547/139‑1 (MQ=255)                                                                                           ccGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTgaga                                                                                           <  1:257566/87‑1 (MQ=255)                                                                                           ccGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTgaga                                                                                           >  2:257566/1‑87 (MQ=255)                                                                                            cGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTg                                      >  2:533892/1‑139 (MQ=255)                                                                                              cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGAc                                    >  2:519881/1‑139 (MQ=255)                                                                                              cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGAc                                    >  2:1098351/1‑139 (MQ=255)                                                                                                           tCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGAt                       <  1:1114335/139‑1 (MQ=255)                                                                                                              cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     <  1:53009/74‑1 (MQ=255)                                                                                                              cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     >  2:53009/1‑74 (MQ=255)                                                                                                              cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     >  2:1101146/1‑74 (MQ=255)                                                                                                              cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCa                                                                                     <  1:1101146/74‑1 (MQ=255)                                                                                                                   ttGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      <  2:416903/52‑1 (MQ=255)                                                                                                                   ttGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                      >  1:416903/1‑52 (MQ=255)                                                                                                                    tGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGaa              >  1:323499/1‑139 (MQ=255)                                                                                                                     gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAg             >  1:828693/1‑139 (MQ=255)                                                                                                                     gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAg             <  2:828693/139‑1 (MQ=255)                                                                                                                           aCCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              >  1:954645/1‑100 (MQ=255)                                                                                                                           aCCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                              <  2:954645/100‑1 (MQ=255)                                                                                                                                cagcaTTGCCACAATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGt  >  1:1047852/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGT  >  NC_000913/3282370‑3282635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑