Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,298,035	T→C	E35G (GAA→GGA)	yraQ ←	putative inner membrane permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,298,035	0	T	C	100.0%	163.3 / NA	46	E35G (GAA→GGA)	yraQ	putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

TTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTG  >  NC_000913/3297903‑3298169                                                                                                                                     |                                                                                                                                       ttCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCAtt                                                                                                                                  <  2:988679/139‑1 (MQ=255)                          tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGAc                                                                                                         >  2:943335/1‑139 (MQ=255)                          tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGAc                                                                                                         >  2:925219/1‑139 (MQ=255)                            gcgTAATCCAACGCCGGCTGACATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACGGTGGGTTTCGGAACCAGTAAACGCATTGCCGGAGTAAGGTCCGCAATTCACTTACCAGAGACCGGCAATGACaa                                                                                                       <  1:788380/139‑1 (MQ=255)                            gcgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACaa                                                                                                       <  2:875946/139‑1 (MQ=255)                                aaTCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACgag                                                                                                   <  2:858207/139‑1 (MQ=255)                                            ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATAc                                                                                                                          >  1:364310/1‑104 (MQ=255)                                            ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATAc                                                                                                                          <  2:364310/104‑1 (MQ=255)                                            ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                                        >  1:572182/1‑138 (MQ=255)                                            ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcg                                                                                        <  2:572182/138‑1 (MQ=255)                                              tGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                                     <  1:171245/139‑1 (MQ=255)                                                ccATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGt                                                                                   <  2:454178/139‑1 (MQ=255)                                                      gTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACGTACCAGAGACCGGCAATGACaa                                                                                                       <  2:289501/113‑1 (MQ=255)                                                      gTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACaa                                                                                                       >  1:289501/1‑113 (MQ=255)                                                        tAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTccacca                                                                           <  1:420123/139‑1 (MQ=255)                                                          gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                                    >  1:760897/1‑80 (MQ=255)                                                          gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                                    <  2:760897/80‑1 (MQ=255)                                                          gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACt                                                                         >  1:1204246/1‑139 (MQ=255)                                                          gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATAACAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACt                                                                         >  1:844981/1‑139 (MQ=255)                                                           cATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCAtt                                                                                                                                  <  1:1174182/81‑1 (MQ=255)                                                           cATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCAtt                                                                                                                                  >  2:1174182/1‑81 (MQ=255)                                                               cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATg                                                                    >  1:914594/1‑139 (MQ=255)                                                                 ccTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                  >  1:144096/1‑139 (MQ=255)                                                                 ccTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATggg                                                                  >  1:410462/1‑139 (MQ=255)                                                                                aTTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTgaga                                                   >  1:247006/1‑139 (MQ=255)                                                                                    aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGaa                                               >  2:782111/1‑139 (MQ=255)                                                                                     ccGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAg                                              >  1:892706/1‑139 (MQ=255)                                                                                      cGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGa                                             >  2:524100/1‑139 (MQ=255)                                                                                       gATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGAc                                            <  2:892706/139‑1 (MQ=255)                                                                                            tgtgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAcc                                       <  1:943335/139‑1 (MQ=255)                                                                                              tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCa                                                                                                                        >  2:553551/1‑56 (MQ=255)                                                                                              tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCa                                                                                                                        <  1:553551/56‑1 (MQ=255)                                                                                              tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAg                                     >  2:1015857/1‑139 (MQ=255)                                                                                               gggTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGt                                    <  1:1020939/139‑1 (MQ=255)                                                                                                  tttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCAt                                 >  1:876772/1‑139 (MQ=255)                                                                                                   ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATAc                                                                                                                          >  2:982874/1‑49 (MQ=255)                                                                                                   ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATAc                                                                                                                          <  1:982874/49‑1 (MQ=255)                                                                                                      ggCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                             >  2:360399/1‑139 (MQ=255)                                                                                                                 aCGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGaa                  <  2:1020417/139‑1 (MQ=255)                                                                                                                    cTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATaa               <  2:876772/139‑1 (MQ=255)                                                                                                                        gCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa           <  1:61472/139‑1 (MQ=255)                                                                                                                        gCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa           <  2:410462/139‑1 (MQ=255)                                                                                                                        gCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTa           <  2:144096/139‑1 (MQ=255)                                                                                                                               taAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAAt    >  2:924332/1‑139 (MQ=255)                                                                                                                                 aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTg  >  2:616520/1‑139 (MQ=255)                                                                                                                                 aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTg  >  1:406754/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       TTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTTATGATAATTG  >  NC_000913/3297903‑3298169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑