Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,347,945	T→C	R277R (CGT→CGC)	yhbJ →	adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,347,945	0	T	C	100.0%	232.6 / NA	64	R277R (CGT→CGC)	yhbJ	adaptor protein for GlmZ/GlmY sRNA decay, glucosamine‑6‑phosphate‑regulated, NTPase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/31);  total (33/31)

TGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTAC  >  NC_000913/3347809‑3348074                                                                                                                                         |                                                                                                                                  tGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCAc                                                                                                                                 >  1:1035424/1‑139 (MQ=255)     ggAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGGAGCACCGTTCGGGGTATATTGCAGAGCAACTGGCAGACGCCTTACGCTCGCGCGGTAAAAACGACCAGTCACGCCATCGCAAGCTg                                                                                                                             >  1:1092154/1‑139 (MQ=255)      gAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTgg                                                                                                                            >  1:1003804/1‑139 (MQ=255)       aaaCCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGa                                                                                                                           <  2:1035424/139‑1 (MQ=255)        aaCCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGaa                                                                                                                          >  2:60245/1‑139 (MQ=255)            aacaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAAc                                                                                                                      >  1:349766/1‑139 (MQ=255)             acaacCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACg                                                                                                                     <  2:349766/139‑1 (MQ=255)                    tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCa                                                                                                              >  2:227750/1‑139 (MQ=255)                    tAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCa                                                                                                              >  1:1165298/1‑139 (MQ=255)                     aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCAt                                                                                                             >  2:377679/1‑139 (MQ=255)                     aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCAt                                                                                                             <  2:1022160/139‑1 (MQ=255)                     aGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCAt                                                                                                             >  2:140024/1‑139 (MQ=255)                         aCTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTTGAAAAACGTAAACCATGAcc                                                                                                         >  1:937563/1‑139 (MQ=255)                          cTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCg                                                                                                        <  2:643893/139‑1 (MQ=255)                                    gCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAAc                                                                                              >  2:869590/1‑139 (MQ=255)                                                aCCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATcac                                                                                  >  2:113209/1‑139 (MQ=255)                                                   ggcggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaa                                                                               <  1:1219723/139‑1 (MQ=255)                                                     cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAAt                                                                                     <  2:379606/131‑1 (MQ=255)                                                     cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAAt                                                                                     >  1:379606/1‑131 (MQ=255)                                                     cggGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCacaaaca                                                                             >  1:1208286/1‑139 (MQ=255)                                                           gCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg                                                                       <  1:869590/139‑1 (MQ=255)                                                           gCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTgg                                                                       <  1:113209/139‑1 (MQ=255)                                                            cACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTggg                                                                      <  1:377679/139‑1 (MQ=255)                                                               cGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcat                                                                   >  1:1098758/1‑139 (MQ=255)                                                                gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  2:1098758/139‑1 (MQ=255)                                                                gTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  2:1003804/139‑1 (MQ=255)                                                                     gtgtATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCatgcatgc                                                             <  1:1029257/139‑1 (MQ=255)                                                                        tataTTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGccca                                                          <  1:227750/139‑2 (MQ=255)                                                                             tGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAATAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTg                                                     >  1:1007744/1‑139 (MQ=255)                                                                                  agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  >  2:343248/1‑121 (MQ=255)                                                                                  agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  1:343248/121‑1 (MQ=255)                                                                                  agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                                >  1:686936/1‑139 (MQ=255)                                                                                  agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                                >  2:110340/1‑139 (MQ=255)                                                                                  agCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg                                                >  1:500656/1‑139 (MQ=255)                                                                                    cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGtt                                                                                          >  1:1015184/1‑95 (MQ=255)                                                                                    cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGtt                                                                                          <  2:1015184/95‑1 (MQ=255)                                                                                    cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  <  2:948056/119‑1 (MQ=255)                                                                                    cAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGgcatg                                                                  >  1:948056/1‑119 (MQ=255)                                                                                     aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg                                             <  1:140024/139‑1 (MQ=255)                                                                                     aaCTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAg                                             <  1:60245/139‑1 (MQ=255)                                                                                       cTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCt                                           >  1:721189/1‑139 (MQ=255)                                                                                              actactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa                                    >  1:180698/1‑139 (MQ=255)                                                                                              actactTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGaa                                    <  2:721189/139‑1 (MQ=255)                                                                                                    tCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATg                              >  2:50191/1‑139 (MQ=255)                                                                                                     ccGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGc                             >  1:892815/1‑139 (MQ=255)                                                                                                          cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGaaa                                                                                                                         >  1:1051931/1‑42 (MQ=255)                                                                                                          cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGaaa                                                                                                                         <  2:1051931/42‑1 (MQ=255)                                                                                                          cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAGCCATGACCGTCAAGCa                                                                                                 >  2:485471/1‑66 (MQ=255)                                                                                                          cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAGCCATGACCGTCAAGCa                                                                                                 <  1:485471/66‑1 (MQ=255)                                                                                                          cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           <  1:189333/88‑1 (MQ=255)                                                                                                          cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           >  2:189333/1‑88 (MQ=255)                                                                                                          cgcgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGt                        >  1:1070699/1‑139 (MQ=255)                                                                                                            cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           <  1:484070/86‑1 (MQ=255)                                                                                                            cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAg                                                                           >  2:484070/1‑86 (MQ=255)                                                                                                            cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGttt                      <  2:500656/139‑1 (MQ=255)                                                                                                            cgcgGTAAAAACGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGttt                      <  1:254212/139‑1 (MQ=255)                                                                                                                     aaCGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGa             >  2:1162576/1‑139 (MQ=255)                                                                                                                      aCGTCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGaa            <  1:185820/139‑1 (MQ=255)                                                                                                                         tCCAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTg         <  2:1070699/139‑1 (MQ=255)                                                                                                                           cAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGaa            >  1:128494/1‑134 (MQ=255)                                                                                                                           cAGTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGaa            <  2:128494/134‑1 (MQ=255)                                                                                                                             gTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTg                                                                                         >  2:360774/1‑55 (MQ=255)                                                                                                                             gTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTg                                                                                         <  1:360774/55‑1 (MQ=255)                                                                                                                             gTCACGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGctct     <  2:1165298/139‑1 (MQ=255)                                                                                                                                aCGCCATCGCACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTAc  <  1:50191/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  TGCTGGAAACCAACAACCGTAGCTACTTGACGGTCGCCATTGGTTGTACCGGCGGGAAGCACCGTTCGGTGTATATTGCAGAGCAACTGGCAGACTACTTCCGCTCGCGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTAC  >  NC_000913/3347809‑3348074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑