Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,478,592	C→T	*67* (TAG→TAA)	yheV ←	DUF2387 family putative metal‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,478,592	0	C	T	100.0%	177.8 / NA	55	*67* (TAG→TAA)	yheV	DUF2387 family putative metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

GATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACAT  >  NC_000913/3478458‑3478723                                                                                                                                       |                                                                                                                                    gATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGt                                                                                                                                 <  2:6142/139‑1 (MQ=255)  aTAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTc                                                                                                                                >  1:1051354/1‑139 (MQ=255)      gCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGa                                                                                                                            >  2:933016/1‑139 (MQ=255)         aGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGa                                                                                                                         >  2:383837/1‑139 (MQ=255)                     aGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             <  2:1099592/139‑1 (MQ=255)                       gTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             >  2:70848/1‑137 (MQ=255)                       gTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATc                                                                                                             <  1:70848/137‑1 (MQ=255)                             tGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTc                                                                                                     <  1:160681/139‑1 (MQ=255)                                 aCTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCt                                                                                                 >  1:976290/1‑139 (MQ=255)                                            atCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTg                                                                                      >  1:955384/1‑139 (MQ=255)                                             tctcCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGa                                                                                     <  1:933016/139‑1 (MQ=255)                                                      cATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGct                                                                            >  2:588265/1‑139 (MQ=255)                                                         gggAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGcttct                                                                         >  2:96991/1‑139 (MQ=255)                                                              gaaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTc                                                                    >  2:1085863/1‑139 (MQ=255)                                                              gaaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTGAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTc                                                                    >  1:750890/1‑139 (MQ=255)                                                               aaTAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCt                                                                   >  2:972049/1‑139 (MQ=255)                                                                 tAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATcc                                                                                                                  >  1:256176/1‑90 (MQ=255)                                                                 tAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATcc                                                                                                                  <  2:256176/90‑1 (MQ=255)                                                                    tGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATc                                                                                   >  2:220333/1‑118 (MQ=255)                                                                    tGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATc                                                                                   <  1:220333/118‑1 (MQ=255)                                                                    tGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCt                                                                   >  1:646089/1‑134 (MQ=255)                                                                    tGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCt                                                                   <  2:646089/134‑1 (MQ=255)                                                                     ggCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                             <  1:588265/139‑1 (MQ=255)                                                                     ggCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtct                                                             <  2:955384/139‑1 (MQ=255)                                                                      gcgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATcc                                                                                                                  >  2:357140/1‑85 (MQ=255)                                                                      gcgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATcc                                                                                                                  <  1:357140/85‑1 (MQ=255)                                                                      gcgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTtctc                                                            >  2:519502/1‑139 (MQ=255)                                                                       cgcAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCg                                                           <  2:882658/139‑1 (MQ=255)                                                                          aGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCAt                                                        <  2:976290/139‑1 (MQ=255)                                                                            aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                      <  1:442132/139‑1 (MQ=255)                                                                            aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                      <  1:96991/139‑1 (MQ=255)                                                                            aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                      <  2:439348/139‑1 (MQ=255)                                                                            aTTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCt                                                      >  2:442132/1‑139 (MQ=255)                                                                              tGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGa                                                    <  2:750890/139‑1 (MQ=255)                                                                                   cGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                               <  2:1051354/139‑1 (MQ=255)                                                                                      aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCgg                                                                                                                             <  1:1109750/58‑1 (MQ=255)                                                                                      aaTTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCgg                                                                                                                             >  2:1109750/1‑58 (MQ=255)                                                                                        ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                               <  2:120124/134‑1 (MQ=255)                                                                                        ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                               >  1:120124/1‑134 (MQ=255)                                                                                        ttCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACt                                          >  1:704573/1‑139 (MQ=255)                                                                                          cTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTa                                        <  2:704573/139‑1 (MQ=255)                                                                                           tGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTaa                                       >  1:478298/1‑139 (MQ=255)                                                                                            gCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAac                                      <  2:478298/139‑1 (MQ=255)                                                                                                  gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACAt                                  <  1:888275/137‑1 (MQ=255)                                                                                                  gtgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACAt                                  >  2:888275/1‑137 (MQ=255)                                                                                                   tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                               <  2:541525/123‑1 (MQ=255)                                                                                                   tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTcc                                               >  1:541525/1‑123 (MQ=255)                                                                                                   tgtACTCACTAGCTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTca                               >  1:1081788/1‑139 (MQ=255)                                                                                                               cTTAAAAAAACTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAAcaatatcaata                   >  2:174734/1‑139 (MQ=255)                                                                                                                  aaaaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTc                                                >  1:49250/1‑107 (MQ=255)                                                                                                                  aaaaaaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTc                                                <  2:49250/107‑1 (MQ=255)                                                                                                                      aaaCTCGGCGCATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTAtttt            >  2:213273/1‑139 (MQ=255)                                                                                                                               gcATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCcaca   <  1:31563/139‑1 (MQ=255)                                                                                                                                cATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACAt  <  1:519502/139‑1 (MQ=255)                                                                                                                                cATATCGTTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACAt  <  1:972049/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    GATAGGCCAGGCTGACCACCAGGTCTTTTGCTACTTTCATGATATCTCCTGAGCATGGGAAGAATAGTGGCGCAGATTGTAGCGGAATTCTGCAGCCGTGTACTCACTAGCTTAAAAAAACTCGGCGCATATCGCTAGTCCGGATGAAAAATCCCGATCACTTGCTCATCTTTGCGAACGTGATCGCGGGCTTCTTTGTCTGCTTCTCGCATCTGATGTCCGCACTTAACACATTCAACAATATCAATATTATTTTCGCGCCACAT  >  NC_000913/3478458‑3478723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑