Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,545,565	G→A	*228* (TAG→TAA)	gntX →	DNA catabolic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,545,565	0	G	A	100.0%	207.0 / NA	63	*228* (TAG→TAA)	gntX	DNA catabolic protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/35);  total (28/35)

CCCGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGA  >  NC_000913/3545431‑3545685                                                                                                                                       |                                                                                                                         cccGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGcc                                                                                                                      >  2:314561/1‑139 (MQ=255)        aaGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                               <  2:476458/139‑1 (MQ=255)         aGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                              <  2:458540/139‑1 (MQ=255)          ggTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                              <  2:465363/138‑1 (MQ=255)          ggTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatgat                                                                                                              >  1:465363/1‑138 (MQ=255)            tCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCGGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                           <  1:702747/139‑1 (MQ=255)             cGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                          <  2:308750/139‑1 (MQ=255)               ccATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGt                                                                                                        >  1:1148477/1‑139 (MQ=255)                             tgGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTa                                                                                          >  2:992236/1‑139 (MQ=255)                                    tgTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGt                                                                                   <  1:204821/139‑1 (MQ=255)                                    tgTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGt                                                                                   <  2:1148477/139‑1 (MQ=255)                                     gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                 >  2:247387/1‑124 (MQ=255)                                     gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATaa                                                                                                 <  1:247387/124‑1 (MQ=255)                                     gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                  <  1:283649/139‑1 (MQ=255)                                     gTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                  >  2:283649/1‑139 (MQ=255)                                      tCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCa                                                                                 <  1:1199371/139‑1 (MQ=255)                                         ttACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACt                                                                              >  2:961204/1‑139 (MQ=255)                                           accaccGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAt                                                                            <  2:554937/139‑1 (MQ=255)                                             caccGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTa                                                                          <  1:681958/139‑1 (MQ=255)                                                cGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       <  1:314561/139‑1 (MQ=255)                                                cGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       <  1:961204/139‑1 (MQ=255)                                                cGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       <  2:729311/139‑1 (MQ=255)                                                       aCCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                               <  1:26468/92‑1 (MQ=255)                                                       aCCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                               >  2:26468/1‑92 (MQ=255)                                                         cGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       >  1:656797/1‑130 (MQ=255)                                                         cGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       <  2:656797/130‑1 (MQ=255)                                                          gTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAAcc                                                                                               <  1:81149/105‑1 (MQ=255)                                                          gTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAAcc                                                                                               >  2:81149/1‑105 (MQ=255)                                                             gCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCAAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATc                                                          >  2:590534/1‑139 (MQ=255)                                                              cAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                               <  2:1124254/85‑1 (MQ=255)                                                              cAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                               >  1:1124254/1‑85 (MQ=255)                                                                gagaTTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGt                                                       <  1:535412/139‑1 (MQ=255)                                                                     tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAgcca                                                                                 <  2:927028/108‑4 (MQ=255)                                                                     tGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGCCa                                                                                 >  1:927028/1‑108 (MQ=255)                                                                             tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                  >  2:831761/1‑99 (MQ=255)                                                                             tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACt                                                                <  1:568122/117‑1 (MQ=255)                                                                             tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACt                                                                >  2:568122/1‑117 (MQ=255)                                                                             tGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGGCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTc                                                                                  <  1:831761/99‑1 (MQ=255)                                                                                 tttACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                               <  2:1107189/66‑1 (MQ=255)                                                                                 tttACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCgatga                                                                                                               >  1:1107189/1‑66 (MQ=255)                                                                                         aTGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       >  2:995352/1‑98 (MQ=255)                                                                                         aTGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       <  1:995352/98‑1 (MQ=255)                                                                                               cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       <  1:332821/92‑1 (MQ=255)                                                                                               cggcggCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGc                                                                       >  2:332821/1‑92 (MQ=255)                                                                                                    gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                          <  2:624294/52‑1 (MQ=255)                                                                                                    gcTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                          >  1:624294/1‑52 (MQ=255)                                                                                                      tGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAActgct                 >  1:553123/1‑139 (MQ=255)                                                                                                       gTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATgg                                                                                                            >  1:664976/1‑47 (MQ=255)                                                                                                       gTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATgg                                                                                                            <  2:664976/47‑1 (MQ=255)                                                                                                                tGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAtt                                                                           <  2:1150817/71‑1 (MQ=255)                                                                                                                tGGTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAtt                                                                           >  1:1150817/1‑71 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                           <  2:380342/37‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                           >  2:1167448/1‑37 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                           >  1:380342/1‑37 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATggg                                                                                                           <  1:1167448/37‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                          >  1:141685/1‑38 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGc                                                                                                          <  2:141685/38‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTaaa                                                                                        >  2:219591/1‑56 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTaaa                                                                                        <  1:219591/56‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAt                                                                            >  2:301095/1‑68 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTAt                                                                            <  1:301095/68‑1 (MQ=255)                                                                                                                  gTGCCTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGga     >  1:389785/1‑139 (MQ=255)                                                                                                                     ccTTTGTCGAACCTTGTAAAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGgaaga  <  2:389785/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                         CCCGTGCAAGGTCGCCATATGGTGATTGTGGATGATGTCGTTACCACCGGAAGTACCGTCGCAGAGATTGCGCAGTTGCTTTTACGCAATGGTGCGGCGGCTGTCCAGGTCTGGTGCCTTTGTCGAACCTTGTAGAGCCTCGATGATGGGCGTATTATAACCAACTAAAATAGTCAACTATTAGGCCATTACTATGATCCGTATTTCCGATGCTGCACAAGCGCACTTTGCCAAACTGCTGGCAAATCAGGAAGA  >  NC_000913/3545431‑3545685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑