Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,913	C→T	A57T (GCA→ACA)	waaH ←	LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,913	0	C	T	100.0%	125.4 / NA	39	A57T (GCA→ACA)	waaH	LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/19);  total (20/19)

ACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGG  >  NC_000913/3789789‑3790045                                                                                                                             |                                                                                                                                     aCTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTa                                                                                                                        >  1:1111719/1‑139 (MQ=255)     cATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTc                                                                                                                    >  1:400395/1‑139 (MQ=255)                tCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTAt                                                                                                         <  2:665231/139‑1 (MQ=255)                        aGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGaa                                                                                                 <  2:400395/139‑1 (MQ=255)                             cATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCACCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATc                                                                                            >  1:848409/1‑139 (MQ=255)                              atatTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGAGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCg                                                                                           >  1:738893/1‑139 (MQ=255)                               tatTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCACCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGt                                                                                          <  2:848409/139‑1 (MQ=255)                                  ttGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTtaa                                                                                       >  2:612365/1‑139 (MQ=255)                                   tGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTtaat                                                                                      <  1:612365/139‑1 (MQ=255)                                        gTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTa                                                                                                                                   >  2:590566/1‑89 (MQ=255)                                        gTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTa                                                                                                                                   <  1:590566/89‑1 (MQ=255)                                                ttCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTcc                                                                         >  2:321031/1‑139 (MQ=255)                                                    aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTa                                                                                                          >  1:1145808/1‑102 (MQ=255)                                                    aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTa                                                                                                          <  2:1145808/102‑1 (MQ=255)                                                    aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag                                                                     <  2:954027/139‑1 (MQ=255)                                                    aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag                                                                     <  2:684527/139‑1 (MQ=255)                                                    aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagaa                                                                     <  2:738893/139‑2 (MQ=255)                                                          cGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACttt                                                                                                                            <  1:353134/78‑1 (MQ=255)                                                          cGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACttt                                                                                                                            >  2:353134/1‑78 (MQ=255)                                                          cGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTcc                                                               >  2:928283/1‑139 (MQ=255)                                                                cgcgCCACCGATGCGTCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGaa                                                                                                 <  1:541931/99‑1 (MQ=255)                                                                cgcgCCACCGATGCGTCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGaa                                                                                                 >  2:541931/1‑99 (MQ=255)                                                                 gcgcCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTg                                                        <  1:928283/139‑1 (MQ=255)                                                                  cgcCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag                                                                     <  1:613189/125‑1 (MQ=255)                                                                  cgcCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag                                                                     >  2:613189/1‑125 (MQ=255)                                                                     cACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTa                                                    >  2:737579/1‑139 (MQ=255)                                                                       ccGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATg                                                                               >  1:1070714/1‑110 (MQ=255)                                                                       ccGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATg                                                                               <  2:1070714/110‑1 (MQ=255)                                                                          aTGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAAtt                                                                                                           <  2:115255/79‑1 (MQ=255)                                                                          aTGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAAtt                                                                                                           >  1:115255/1‑79 (MQ=255)                                                                               ccAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCCATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGAtt                                          <  1:737579/139‑1 (MQ=255)                                                                                    aTTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATa                                     >  2:383064/1‑139 (MQ=255)                                                                                        gCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAg                                 >  1:10721/1‑139 (MQ=255)                                                                                             aTGCAACAAACGAACGTGCGGATAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCgg                            >  1:1098526/1‑139 (MQ=255)                                                                                                                  aTAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATtatat       >  2:909434/1‑139 (MQ=255)                                                                                                                   tAGTTTTCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATtatata      <  2:10721/139‑1 (MQ=255)                                                                                                                       tttCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACgg  <  2:1111719/139‑1 (MQ=255)                                                                                                                        ttCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag                                                                     <  2:1001467/71‑1 (MQ=255)                                                                                                                        ttCTGTGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCagag                                                                     >  1:1001467/1‑71 (MQ=255)                                                                                                                             |                                                                                                                                     ACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGG  >  NC_000913/3789789‑3790045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑