Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,819,488 C→T H205H (CAC→CAT
*561* (TAG→TAA
yicG →
ligB ←
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,819,4880CT100.0% 181.1 / NA 57H205H (CAC→CAT
*561* (TAG→TAA
yicG
ligB
UPF0126 family inner membrane protein
DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

CATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCTC  >  NC_000913/3819353‑3819622
                                                                                                                                       |                                                                                                                                      
cATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTaa                                                                                                                                     <  1:1174532/139‑1 (MQ=255)
          cAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                           >  1:1118190/1‑139 (MQ=255)
          cAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                           >  2:379608/1‑139 (MQ=255)
             cacTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCtg                                                                                                                        >  1:347115/1‑139 (MQ=255)
                      tCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATctgct                                                                                                               <  1:89013/139‑1 (MQ=255)
                          aCCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                           >  1:356649/1‑139 (MQ=255)
                          aCCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTGTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGc                                                                                                           >  2:772758/1‑139 (MQ=255)
                              tgatgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                           >  1:226678/1‑119 (MQ=255)
                              tgatgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAAc                                                                                                                           <  2:226678/119‑1 (MQ=255)
                               gatgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      <  2:1118190/139‑1 (MQ=255)
                               gatgTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      <  1:379608/139‑1 (MQ=255)
                                    ggTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCaa                                                                                                 >  1:812974/1‑139 (MQ=255)
                                         ttATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:347115/139‑1 (MQ=255)
                                         ttATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:812974/139‑1 (MQ=255)
                                         ttATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:356649/139‑1 (MQ=255)
                                         ttATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:729113/139‑1 (MQ=255)
                                         ttATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:523846/139‑1 (MQ=255)
                                           aTCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCa                                                                                          <  1:772758/139‑1 (MQ=255)
                                             ctcCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                              <  2:744181/101‑1 (MQ=255)
                                             ctcCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCaa                                                                                                                              >  1:744181/1‑101 (MQ=255)
                                                cACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGcttc                                                                                     >  2:524872/1‑139 (MQ=255)
                                                 aCCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATc                                                                                                                   <  2:33491/108‑1 (MQ=255)
                                                 aCCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATc                                                                                                                   >  1:33491/1‑108 (MQ=255)
                                                   ccTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGGAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTg                                                                                  >  1:663319/1‑139 (MQ=255)
                                                     tGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGAt                                                                                >  1:132110/1‑139 (MQ=255)
                                                       gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTaa                                                                                                                                     <  2:731948/84‑1 (MQ=255)
                                                       gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTaa                                                                                                                                     >  1:731948/1‑84 (MQ=255)
                                                       gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      >  2:895139/1‑115 (MQ=255)
                                                       gTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGcagcca                                                                                                      <  1:895139/115‑1 (MQ=255)
                                                             ggCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAgg                                                                                                                                   <  1:1136004/80‑1 (MQ=255)
                                                             ggCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAgg                                                                                                                                   >  2:1136004/1‑80 (MQ=255)
                                                             ggCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCAt                                                                        >  1:174685/1‑139 (MQ=255)
                                                             ggCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCAt                                                                        >  1:389284/1‑139 (MQ=255)
                                                                         cGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCAtt                                                            >  2:150437/1‑139 (MQ=255)
                                                                          gCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTc                                                           <  2:313520/139‑1 (MQ=255)
                                                                                 ggCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGTTTTGCGCATTTTCCTTCCATTCAATAAcc                                                    <  2:781388/139‑1 (MQ=255)
                                                                                        cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATctg                                                                                                                 >  2:652839/1‑71 (MQ=255)
                                                                                        cGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATctg                                                                                                                 <  1:652839/71‑1 (MQ=255)
                                                                                         gCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgc                                            <  2:951545/139‑1 (MQ=255)
                                                                                               aGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTcc                                      <  2:1024720/139‑1 (MQ=255)
                                                                                                    ggATTACCGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATc                                 >  1:1107657/1‑139 (MQ=255)
                                                                                                          ccGGTCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTcc                           >  1:1086946/1‑139 (MQ=255)
                                                                                                              tCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                               >  2:1075547/1‑99 (MQ=255)
                                                                                                              tCTTTTACTACAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTttccttcc                                                               <  1:1075547/99‑1 (MQ=255)
                                                                                                                   tactacCGCCACGAAGGTCTTTAAGGTTCAAAACCTGTGATCTTCTGGGAAGCCAGCTAACTTCCCAGCTTCTTGATTTGCCCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAgct                  <  1:135007/139‑1 (MQ=255)
                                                                                                                        cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgc             >  2:175061/1‑139 (MQ=255)
                                                                                                                        cAGCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGAGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCtgctgc             >  2:735106/1‑139 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            <  2:327000/58‑1 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGcc                                                                                            >  1:327000/1‑58 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                           >  1:1050820/1‑107 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                           >  2:981304/1‑107 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                           <  2:1050820/107‑1 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGgcg                                           <  1:981304/107‑1 (MQ=255)
                                                                                                                          gCCACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCa           >  2:857735/1‑139 (MQ=255)
                                                                                                                           ccACGAAGGACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAg          >  1:878485/1‑139 (MQ=255)
                                                                                                                                  ggACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCt   <  1:524872/139‑1 (MQ=255)
                                                                                                                                   gACATTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCTc  <  1:857735/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CATCGCGCTGCAACACTATGTTTCTAACCATGATGTGGTAATTATCTCCACCCTGGTATTCGGCTTTTTTGCCCGCTTACTGGCGCTACGCCTTAAGCTGGGATTACCGGTCTTTTACTACAGCCACGAAGGACACTAAGGTTCAAAACCTGTGATCTGCTGGGCAGCCAGCCAACTGCCCAGCTTCTTGATTTGCGCATTTTCCTTCCATTCAATAACCTGTCTGGCGCGTCCCGATCCAGTCCCCGGCAGCTGCTGCCAGAACTGCTC  >  NC_000913/3819353‑3819622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: