Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,937,168 G→A *297* (TAG→TAA)  rbsB → D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,937,1680GA100.0% 155.0 / NA 48*297* (TAG→TAA) rbsBD‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/30);  total (18/30)

CAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAAC  >  NC_000913/3937033‑3937302
                                                                                                                                       |                                                                                                                                      
cAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAAttt                                                                                                                                     >  1:848313/1‑139 (MQ=255)
    cTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                                 <  2:729504/139‑1 (MQ=255)
    cTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                                 <  1:427271/139‑1 (MQ=255)
      agcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCa                                                                                                                               <  1:1130844/139‑1 (MQ=255)
       gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                              <  2:401244/139‑1 (MQ=255)
             aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                                        <  1:134430/139‑1 (MQ=255)
             aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                                        <  2:568897/139‑1 (MQ=255)
             aCTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                                        >  1:379232/1‑139 (MQ=255)
               tATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATg                                                                                                                      >  2:139755/1‑139 (MQ=255)
                 tCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                                    <  2:262714/139‑1 (MQ=255)
                     tCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGa                                                                                                                >  2:1186218/1‑139 (MQ=255)
                       aGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATg                                                                                                              <  2:379232/139‑1 (MQ=255)
                         cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                                            <  1:431837/139‑1 (MQ=255)
                           aCCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCa                                                                                                                               >  2:714019/1‑118 (MQ=255)
                           aCCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCa                                                                                                                               <  1:714019/118‑1 (MQ=255)
                                 tCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATaaa                                                                                                    >  1:749693/1‑139 (MQ=255)
                                  cAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAAt                                                                                                   <  1:611611/139‑1 (MQ=255)
                                      ttGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGt                                                                                               <  1:139755/139‑1 (MQ=255)
                                       tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                                 >  1:624406/1‑104 (MQ=255)
                                       tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAAt                                                                                                                                 <  2:624406/104‑1 (MQ=255)
                                       tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCt                                                                                                                  <  1:161308/119‑1 (MQ=255)
                                       tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCt                                                                                                                  >  2:161308/1‑119 (MQ=255)
                                       tGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTc                                                                                              >  1:50853/1‑139 (MQ=255)
                                         gcgcGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCAt                                                                                            <  1:1068031/139‑1 (MQ=255)
                                          cgcgAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATc                                                                                           >  1:470218/1‑139 (MQ=255)
                                                  gCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATg                                                                                   <  1:454177/139‑1 (MQ=255)
                                                   cGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                                  <  1:126521/139‑1 (MQ=255)
                                                        aaaCCGCAGATAAAGTGGTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATCACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtc                                                                             <  2:749693/139‑2 (MQ=255)
                                                        aaaCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                             <  2:470218/139‑1 (MQ=255)
                                                        aaaCCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                             <  1:119695/139‑1 (MQ=255)
                                                             gCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAata                                                                        <  1:143343/139‑1 (MQ=255)
                                                                           gAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                             <  1:899440/120‑1 (MQ=255)
                                                                           gAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                             >  2:899440/1‑120 (MQ=255)
                                                                                 cGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                                        <  2:968054/71‑1 (MQ=255)
                                                                                 cGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                                        >  1:968054/1‑71 (MQ=255)
                                                                                   agaAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                                  <  1:1186218/139‑1 (MQ=255)
                                                                                                                     aCTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATcc                <  2:848313/139‑1 (MQ=255)
                                                                                                                        ggTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGa             >  2:236584/1‑139 (MQ=255)
                                                                                                                         gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGAc                                                                                        >  1:742044/1‑63 (MQ=255)
                                                                                                                         gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGAc                                                                                        <  2:742044/63‑1 (MQ=255)
                                                                                                                         gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGa                     >  2:1120182/1‑130 (MQ=255)
                                                                                                                         gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGa                     <  1:1120182/130‑1 (MQ=255)
                                                                                                                         gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGaa            <  1:912953/139‑1 (MQ=255)
                                                                                                                         gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGaa            >  1:437472/1‑139 (MQ=255)
                                                                                                                         gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGaa            >  2:912953/1‑139 (MQ=255)
                                                                                                                          ttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAat           <  2:437472/139‑1 (MQ=255)
                                                                                                                                aGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGc       >  1:873366/1‑137 (MQ=255)
                                                                                                                                aGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGc       <  2:873366/137‑1 (MQ=255)
                                                                                                                                   agtaatTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAAc  >  2:453955/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CAAACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAATATGCAAAAC  >  NC_000913/3937033‑3937302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑