Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,015	T→C	W329R (TGG→CGG)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,015	0	T	C	100.0%	144.1 / NA	42	W329R (TGG→CGG)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/22);  total (20/22)

AGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG  >  NC_000913/3969881‑3970148                                                                                                                                       |                                                                                                                                       aGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                    <  1:433240/139‑1 (MQ=255) aGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCgggg                                                                                                                                    <  1:357802/139‑1 (MQ=255)  gAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGc                                                                                                                                   >  1:1078210/1‑139 (MQ=255)        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCAtt                                                                                                                                <  1:1168581/136‑1 (MQ=255)        ggCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCAtt                                                                                                                                >  2:1168581/1‑136 (MQ=255)                    cacCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGAt                                                                                                                 <  2:123199/139‑1 (MQ=255)                                 ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTc                                                                                                    >  1:28389/1‑139 (MQ=255)                                 ggTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTc                                                                                                    >  1:832108/1‑139 (MQ=255)                                    ccAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCa                                                                                                 <  2:600532/139‑1 (MQ=255)                                             gATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACccgc                                                                                        >  1:372585/1‑139 (MQ=255)                                                    gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                 <  2:385770/139‑1 (MQ=255)                                                    gTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCt                                                                                 <  2:28389/139‑1 (MQ=255)                                                                aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                     >  1:916709/1‑139 (MQ=255)                                                                             tACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                      <  1:435895/125‑1 (MQ=255)                                                                             tACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                      >  2:435895/1‑125 (MQ=255)                                                                               cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAgc                                                      >  2:288911/1‑139 (MQ=255)                                                                               cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg                                                                                                                          >  1:146695/1‑70 (MQ=255)                                                                               cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑gg                                                                                                                          <  2:146695/70‑1 (MQ=255)                                                                                gCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAgcg                                                     >  2:515955/1‑139 (MQ=255)                                                                                 ccGGAAGAGCCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                              <  2:781884/113‑1 (MQ=255)                                                                                 ccGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGa                                                                              >  1:781884/1‑113 (MQ=255)                                                                                          ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCt                                           <  2:832108/139‑1 (MQ=255)                                                                                          ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGACGTTGCTCGAAAt                                                                           <  2:637373/107‑1 (MQ=255)                                                                                          ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGACGTTGCTCGAAAt                                                                           >  1:637373/1‑107 (MQ=255)                                                                                              tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCa                                                   >  2:1145081/1‑127 (MQ=255)                                                                                              tAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCa                                                   <  1:1145081/127‑1 (MQ=255)                                                                                                  aCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCtt                                   >  1:324290/1‑139 (MQ=255)                                                                                                        tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                    >  2:745312/1‑100 (MQ=255)                                                                                                        tAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                    <  1:745312/100‑1 (MQ=255)                                                                                                             cACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAgaga                        >  2:85606/1‑139 (MQ=255)                                                                                                                     gCCTTCCTGATGATTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtgt                >  2:963521/1‑139 (MQ=255)                                                                                                                            tgatgaTTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa         <  1:515955/139‑1 (MQ=255)                                                                                                                            tgatgaTTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa         <  2:909310/139‑1 (MQ=255)                                                                                                                            tgatgaTTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa         <  2:1000076/139‑1 (MQ=255)                                                                                                                              atgatTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                >  1:297650/1‑66 (MQ=255)                                                                                                                              atgatTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTc                                                                                <  2:297650/66‑1 (MQ=255)                                                                                                                              atgatTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTact       <  2:372585/139‑1 (MQ=255)                                                                                                                                 atTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac    <  1:85606/139‑1 (MQ=255)                                                                                                                                 atTATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgac    <  2:324290/139‑1 (MQ=255)                                                                                                                                  ttATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt          <  1:1065149/132‑1 (MQ=255)                                                                                                                                  ttATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt          >  2:1065149/1‑132 (MQ=255)                                                                                                                                   tATGCGGGGCATTGTCGGGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactgactg  >  2:920511/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       AGAATCGGGCCATGTTAAACACCCTGAATGTTGGTCCAACCCTGGATCCGCGTTTTCAGACCTATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑GGGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTG  >  NC_000913/3969881‑3970148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑