Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,024,336	G→A	*261* (TAG→TAA)  L162L (CTC→CTT)	tatD → rfaH ←	quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase transcription antitermination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,024,336	0	G	A	100.0%	197.9 / NA	62	*261* (TAG→TAA)  L162L (CTC→CTT)	tatD rfaH	quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/34);  total (28/34)

CGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGT  >  NC_000913/4024208‑4024467                                                                                                                                 |                                                                                                                                    cGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGa                                                                                                                           <  1:410888/139‑1 (MQ=255)  ggCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGaa                                                                                                                          >  1:1086934/1‑139 (MQ=255)      cAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                      <  1:148532/139‑1 (MQ=255)      cAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                      <  2:1010108/139‑1 (MQ=255)        aCGAGCCAGCCCATCTGCCCCATATTTTGTAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:1018336/139‑1 (MQ=255)         cGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTa                                                                                                                   >  2:934819/1‑139 (MQ=255)          gAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                  <  1:141171/139‑1 (MQ=255)             ccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttct                                                                                                               >  2:626705/1‑139 (MQ=255)                  ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          <  1:1206943/139‑1 (MQ=255)                  ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                          <  1:272189/139‑1 (MQ=255)                    aTCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACt                                                                                                        <  1:220533/139‑1 (MQ=255)                     tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtg                                                                                                       <  1:244929/139‑1 (MQ=255)                      cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttcttc                                                                                                             >  2:964514/1‑132 (MQ=255)                      cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttcttc                                                                                                             <  1:964514/132‑1 (MQ=255)                           ccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                 <  1:102494/139‑1 (MQ=255)                           ccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                 <  1:934819/139‑1 (MQ=255)                                   tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                                         >  2:222975/1‑139 (MQ=255)                                    gCAACGTATTGCGCACTGGCGTGGAGTAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTg                                                                                                                               <  2:746590/100‑1 (MQ=255)                                    gCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTCGGGATTGCGTTTTAAAGTTTg                                                                                                                               >  1:746590/1‑100 (MQ=255)                                        cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTATtct                                                                                             >  2:31138/1‑130 (MQ=255)                                        cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                             <  1:31138/130‑1 (MQ=255)                                        cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    >  2:505711/1‑139 (MQ=255)                                              gcgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAt                                                                              <  2:1086934/139‑1 (MQ=255)                                               cgcACTGGTGTGGAGAAGATGCCTCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAtt                                                                             <  2:537910/139‑1 (MQ=255)                                                gcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                            <  1:626705/139‑1 (MQ=255)                                                    tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGTGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    <  2:697993/127‑1 (MQ=255)                                                    tGGCGTGGAGAAGATGCAGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGTGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                    >  1:697993/1‑127 (MQ=255)                                                    tGGCGAGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAACGTTTGCGGCACTCGGTATGCGTCACACTGTGCTTAATCTCTTTATTAATACGATTacgaa                                                                        >  1:537910/1‑135 (MQ=255)                                                                gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  1:574730/83‑1 (MQ=255)                                                                gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    >  2:574730/1‑83 (MQ=255)                                                                 aTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagcg                                                           >  1:559234/1‑139 (MQ=255)                                                                   gCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         >  2:1206450/1‑91 (MQ=255)                                                                   gCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                         <  1:1206450/91‑1 (MQ=255)                                                                        aTGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAt                                                                              >  1:63180/1‑113 (MQ=255)                                                                        aTGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAt                                                                              <  2:63180/113‑1 (MQ=255)                                                                            ctggctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCAt                                                >  1:284023/1‑139 (MQ=255)                                                                                ctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCggg                                            <  2:559234/139‑1 (MQ=255)                                                                                 tgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGt                                           <  1:505711/139‑1 (MQ=255)                                                                                               cTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                        >  1:451783/1‑96 (MQ=255)                                                                                               cTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                        <  2:451783/96‑1 (MQ=255)                                                                                                 aaTGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTc                                         >  1:36697/1‑125 (MQ=255)                                                                                                 aaTGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTc                                         <  2:36697/125‑1 (MQ=255)                                                                                                    gTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    >  1:369944/1‑47 (MQ=255)                                                                                                    gTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                    <  2:369944/47‑1 (MQ=255)                                                                                                      cAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAg                      >  2:53968/1‑139 (MQ=255)                                                                                                       aaaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGc                     >  1:1153101/1‑139 (MQ=255)                                                                                                        aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                             <  1:627989/98‑1 (MQ=255)                                                                                                        aaaCACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                             >  2:627989/1‑98 (MQ=255)                                                                                                              tGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttctt                                                                                                              <  1:828984/43‑1 (MQ=255)                                                                                                              tGTCTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttctt                                                                                                              >  2:828984/1‑43 (MQ=255)                                                                                                               gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAtt                                                                             >  2:61370/1‑75 (MQ=255)                                                                                                               gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAtt                                                                             <  1:61370/75‑1 (MQ=255)                                                                                                                     gATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCt       >  1:904720/1‑139 (MQ=255)                                                                                                                       ttGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                        <  1:392687/72‑1 (MQ=255)                                                                                                                       ttGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                        >  2:392687/1‑72 (MQ=255)                                                                                                                         gCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCgg   <  2:1153101/139‑1 (MQ=255)                                                                                                                         gCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCgg   <  2:284023/139‑1 (MQ=255)                                                                                                                          cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTc                                                     <  2:453772/88‑1 (MQ=255)                                                                                                                          cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTc                                                     >  1:453772/1‑88 (MQ=255)                                                                                                                          cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCg    <  1:808511/137‑1 (MQ=255)                                                                                                                          cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCg    >  2:808511/1‑137 (MQ=255)                                                                                                                          cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt  >  1:1211022/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                    CGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGT  >  NC_000913/4024208‑4024467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑