Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,117,123	G→A	R34C (CGC→TGC)	glpK ←	glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,123	0	G	A	100.0%	266.4 / NA	81	R34C (CGC→TGC)	glpK	glycerol kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/44);  total (37/44)

CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAT  >  NC_000913/4116987‑4117245                                                                                                                                         |                                                                                                                           ccGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACt                                                                                                                          >  2:389094/1‑139 (MQ=255)   gATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGc                                                                                                                        >  2:983085/1‑139 (MQ=255)    aTAGTTGCAATATGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGGTTTGCTCAAATTCGCACTGCg                                                                                                                       >  1:708531/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                       >  1:371829/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                       >  1:909590/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                       >  1:694079/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                       >  2:385586/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                       >  1:1048293/1‑139 (MQ=255)    aTAGCTGCAATTTGATCGGAACTCATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCg                                                                                                                       >  1:733542/1‑139 (MQ=255)     tAGCTGCAATTTGATCGGAACTGTCATCGGCTTTCGCCAGCACTTCTACAAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                      <  2:582796/139‑1 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTTGGTCGTGTTCTACCCACCCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                      <  1:112863/139‑1 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTATCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                      >  2:186232/1‑139 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                      <  2:481942/139‑1 (MQ=255)     tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                      <  1:385586/139‑1 (MQ=255)      aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTATCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                     <  1:186232/139‑1 (MQ=255)      aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                     <  2:604316/139‑1 (MQ=255)      aGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGac                                                                                                                     >  2:1024356/1‑139 (MQ=255)       gCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGaca                                                                                                                    <  2:153592/139‑1 (MQ=255)        cTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGacac                                                                                                                   <  2:27577/139‑1 (MQ=255)         tGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                  <  2:1119989/139‑1 (MQ=255)           cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCt                                                                                                                <  1:954731/139‑1 (MQ=255)           cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCt                                                                                                                >  2:916382/1‑139 (MQ=255)           cAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCt                                                                                                                <  2:1048293/139‑1 (MQ=255)            aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                               <  2:699988/139‑1 (MQ=255)            aaTTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTa                                                                                                               <  2:356643/139‑1 (MQ=255)                 gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGat                                                                                                          <  1:46232/139‑1 (MQ=255)                            atatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGt                                                                                               >  1:608884/1‑139 (MQ=255)                            atatCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGt                                                                                               <  2:492059/139‑1 (MQ=255)                              atCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             <  2:371829/139‑1 (MQ=255)                              atCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             <  1:743113/139‑1 (MQ=255)                              atCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             <  2:694079/139‑1 (MQ=255)                                 ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                  <  2:274989/115‑1 (MQ=255)                                 ggCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACg                                                                                                                  >  1:274989/1‑115 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTAc                                                                                     >  1:386241/1‑139 (MQ=255)                                      tCGCCAGCACTTCTACCAGCGTGGAGCTTTAGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAAGGATATTGGCATCGTGATCCATTAc                                                                                     >  2:681005/1‑139 (MQ=255)                                               cTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACcgcgcg                                                                            <  1:1024356/139‑1 (MQ=255)                                                        gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATAtt                                                                                                       >  2:1062974/1‑103 (MQ=255)                                                        gCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATAtt                                                                                                       <  1:1062974/103‑1 (MQ=255)                                                         cGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtg                                                                  >  1:229210/1‑139 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                 >  2:347299/1‑139 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                 >  1:760224/1‑139 (MQ=255)                                                          gTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCtggtgg                                                                 <  2:386241/139‑1 (MQ=255)                                                             gAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATc                                                                                           <  1:918285/110‑1 (MQ=255)                                                             gAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATc                                                                                           >  2:918285/1‑110 (MQ=255)                                                                       tGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAg                                                    <  2:608884/139‑1 (MQ=255)                                                                         gCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcg                                                  <  2:708531/139‑1 (MQ=255)                                                                          cccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcgc                                                 <  1:347299/139‑1 (MQ=255)                                                                          cccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAgcgc                                                 >  2:201994/1‑139 (MQ=255)                                                                           ccAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCa                                                <  1:983085/139‑1 (MQ=255)                                                                                   ccATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                      <  2:1137383/61‑1 (MQ=255)                                                                                   ccATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGa                                                                                                                      >  1:1137383/1‑61 (MQ=255)                                                                                    cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             >  2:268804/1‑85 (MQ=255)                                                                                    cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             <  1:268804/85‑1 (MQ=255)                                                                                    cATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGatatat                                       >  2:691924/1‑139 (MQ=255)                                                                                        gggTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttttt                                   >  1:951835/1‑139 (MQ=255)                                                                                              tgtTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAg                             <  2:760224/139‑1 (MQ=255)                                                                                                ttCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                           <  1:681005/139‑1 (MQ=255)                                                                                                   tACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGt                            >  1:1022501/1‑135 (MQ=255)                                                                                                   tACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGt                            <  2:1022501/135‑1 (MQ=255)                                                                                                     cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATc                                                                                                 <  2:310089/64‑1 (MQ=255)                                                                                                     cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATc                                                                                                 >  1:310089/1‑64 (MQ=255)                                                                                                     cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                           <  1:1042772/134‑1 (MQ=255)                                                                                                     cccAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTc                           >  2:1042772/1‑134 (MQ=255)                                                                                                        aaCCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             >  1:251813/1‑65 (MQ=255)                                                                                                        aaCCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             <  2:251813/65‑1 (MQ=255)                                                                                                        aaCCTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTaa                   <  2:951835/139‑1 (MQ=255)                                                                                                          ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             >  2:351317/1‑63 (MQ=255)                                                                                                          ccTGGTTTTGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGa                                                                                             <  1:351317/63‑1 (MQ=255)                                                                                                               ttttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc            >  1:819363/1‑139 (MQ=255)                                                                                                               ttttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc            <  2:229210/139‑1 (MQ=255)                                                                                                                 ttGGGTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt          <  1:201994/139‑1 (MQ=255)                                                                                                                   gggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg        <  1:916382/139‑1 (MQ=255)                                                                                                                   gggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAg        <  1:691924/139‑1 (MQ=255)                                                                                                                    ggTAGATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGt       >  1:54116/1‑139 (MQ=255)                                                                                                                        gATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt          <  1:552100/132‑1 (MQ=255)                                                                                                                        gATTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt          >  2:552100/1‑132 (MQ=255)                                                                                                                         aTTTGCTCAAATTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCatat  >  1:468392/1‑139 (MQ=255)                                                                                                                                   aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc            >  1:1100462/1‑119 (MQ=255)                                                                                                                                   aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTccc            <  2:1100462/119‑1 (MQ=255)                                                                                                                                   aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt          >  2:369540/1‑121 (MQ=255)                                                                                                                                   aTTCGCACTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGt          <  1:369540/121‑1 (MQ=255)                                                                                                                                         |                                                                                                                           CCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAACCTGGTTTTGGGTAGATTTGCTCAAATTCGCGCTGCGACACGCTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAT  >  NC_000913/4116987‑4117245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑