Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,148,681	C→T	G529S (GGC→AGC)	eptC ←	LPS heptose I phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,148,681	0	C	T	100.0%	201.0 / NA	63	G529S (GGC→AGC)	eptC	LPS heptose I phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/32);  total (31/32)

ACCTGATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGG  >  NC_000913/4148545‑4148803                                                                                                                                         |                                                                                                                           aCCTGATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCg                                                                                                                          <  1:1032846/139‑1 (MQ=255)        cGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATc                                                                                                                   >  2:47888/1‑139 (MQ=255)            aTAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                               <  1:915963/139‑1 (MQ=255)            aTAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAc                                                                                                               <  1:131168/139‑1 (MQ=255)               gggCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAg                                                                                                            <  2:1150960/139‑1 (MQ=255)                     tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGa                                                                                                                <  1:245694/129‑1 (MQ=255)                     tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGa                                                                                                                >  2:245694/1‑129 (MQ=255)                     tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACTAGGTGTgg                                                                                                      >  1:153498/1‑139 (MQ=255)                     tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                      >  2:163768/1‑139 (MQ=255)                     tgtgtCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTgg                                                                                                      >  1:490110/1‑139 (MQ=255)                          cGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAg                                                                                                 >  1:1182462/1‑139 (MQ=255)                            tAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGtt                                                                                               <  2:1182462/139‑1 (MQ=255)                              aTCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGc                                                                                                                         >  1:568700/1‑111 (MQ=255)                              aTCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGc                                                                                                                         <  2:568700/111‑1 (MQ=255)                               tCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCg                                                                                            >  2:394936/1‑139 (MQ=255)                                   tCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAg                                                                                        <  1:413687/139‑1 (MQ=255)                                     aGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGc                                                                                      <  2:404475/139‑1 (MQ=255)                                       tGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTg                                                                                    >  1:45481/1‑139 (MQ=255)                                            ttttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                              <  2:26831/108‑1 (MQ=255)                                            ttttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                              >  1:26831/1‑108 (MQ=255)                                              ttCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                             <  2:490110/139‑1 (MQ=255)                                                 ttATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGAt                                                                          >  2:149437/1‑139 (MQ=255)                                                         gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTAttt                                                                               >  1:579294/1‑126 (MQ=255)                                                         gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTAttt                                                                               <  2:579294/126‑1 (MQ=255)                                                         gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                             <  2:1038895/128‑1 (MQ=255)                                                         gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                             >  1:1038895/1‑128 (MQ=255)                                                         gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATcc                                                                  >  2:988692/1‑139 (MQ=255)                                                         gTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATcc                                                                  >  2:674795/1‑139 (MQ=255)                                                             ccAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                              >  2:993921/1‑91 (MQ=255)                                                             ccAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAcc                                                                                                              <  1:993921/91‑1 (MQ=255)                                                             ccAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCg                                                              >  1:198816/1‑139 (MQ=255)                                                                 tCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                       <  1:1219419/110‑1 (MQ=255)                                                                 tCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAgg                                                                                       >  2:1219419/1‑110 (MQ=255)                                                                 tCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGaa                                                          >  1:298085/1‑139 (MQ=255)                                                                   cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTa                                                                              <  2:389529/117‑1 (MQ=255)                                                                   cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTa                                                                              <  2:841760/117‑1 (MQ=255)                                                                   cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTa                                                                              >  1:389529/1‑117 (MQ=255)                                                                   cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTa                                                                              >  1:841760/1‑117 (MQ=255)                                                                   cAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAAt                                                        >  1:566226/1‑139 (MQ=255)                                                                           aGTTTCTTTGAACTGAGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGAACAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGTTCAACATCCTGCGAGAAATCACGGGGa                                                >  1:748969/1‑139 (MQ=255)                                                                              ttCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGa                                             <  1:988692/139‑1 (MQ=255)                                                                                          cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGa                                                                                                                >  1:621785/1‑60 (MQ=255)                                                                                          cGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGa                                                                                                                <  2:621785/60‑1 (MQ=255)                                                                                                        aaCGGGTTGGATCGTAACCGTCGTAAGATAAGCTCGACAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAAGATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCca                   >  2:775977/1‑139 (MQ=255)                                                                                                             gTTGGATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCAcagca              >  2:872152/1‑139 (MQ=255)                                                                                                                ggATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                             >  1:1042459/1‑73 (MQ=255)                                                                                                                ggATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTAc                                                                             <  2:1042459/73‑1 (MQ=255)                                                                                                                ggATCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGa           >  2:493468/1‑139 (MQ=255)                                                                                                                  aTCGTAACCGTCGTACGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGa           <  1:139143/137‑1 (MQ=255)                                                                                                                  aTCGTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGa           >  2:139143/1‑137 (MQ=255)                                                                                                                     gTAACCGTCGTAAGATAAGCTCGCCATATCTGACCAGCTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa      <  1:775977/139‑1 (MQ=255)                                                                                                                     gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa      <  1:47888/139‑1 (MQ=255)                                                                                                                     gTAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa      <  2:153498/139‑1 (MQ=255)                                                                                                                      tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa     <  1:163768/139‑1 (MQ=255)                                                                                                                      tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa     <  2:45481/139‑1 (MQ=255)                                                                                                                      tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa     <  2:1054083/139‑1 (MQ=255)                                                                                                                      tAACCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTACTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGaa     <  1:872152/139‑1 (MQ=255)                                                                                                                        aCCGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATg   <  2:566226/139‑1 (MQ=255)                                                                                                                         ccGTCGTAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATgg  <  2:169729/139‑1 (MQ=255)                                                                                                                            tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa      <  1:848179/132‑1 (MQ=255)                                                                                                                            tcgtAAGATAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGa      >  2:848179/1‑132 (MQ=255)                                                                                                                                    tAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                      <  2:548802/76‑1 (MQ=255)                                                                                                                                    tAAGCTCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCa                                                      >  1:548802/1‑76 (MQ=255)                                                                                                                                         |                                                                                                                           ACCTGATCGCCATAGGGCAGTGTGTCGTAATCGATCAGTGCGTTTTTCTTATACGGGTTACCAATCCAGCGGGTAGTTTCTTTGAACTGCGGATTCACCACTGAACGGGTTGGATCGTAACCGTCGTAAGATAAGCCCGCCAAATCTGACCAGGTGTGGATCAGTTCCGCCAGGCTGTATTTACGATCAACATCCTGCGAGAAATCACGGGGATGAGTCGCTTGCCATTTTTCTGACGTCCACAGCAGGAACGGAATGG  >  NC_000913/4148545‑4148803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑