| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,215,740 | A→G | E88G (GAA→GGA) | aceB → | malate synthase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,215,740 | 0 | A | G | 100.0% | 106.9 / NA | 31 | E88G (GAA→GGA) | aceB | malate synthase A |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/19); total (12/19) | |||||||||||
CGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAC > NC_000913/4215607‑4215858 | cGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAcc > 2:288187/1‑139 (MQ=255) aaTAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGAccgcc < 2:1152934/139‑1 (MQ=255) aaaCTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg < 1:251357/139‑1 (MQ=255) cAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAAc < 2:796490/139‑1 (MQ=255) cgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGcc < 2:351934/139‑1 (MQ=255) aTTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCgg < 1:288187/139‑1 (MQ=255) agcagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg < 1:929634/139‑1 (MQ=255) cagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa < 1:155761/139‑1 (MQ=255) gcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg < 2:669133/139‑1 (MQ=255) aGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg > 2:944352/1‑136 (MQ=255) aGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg < 1:944352/136‑1 (MQ=255) aaCGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTc > 2:350688/1‑139 (MQ=255) tGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTagag < 2:628470/110‑1 (MQ=255) tGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTagag > 1:628470/1‑110 (MQ=255) ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa < 1:350688/139‑1 (MQ=255) ccTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa < 2:83724/139‑1 (MQ=255) tttCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACcgccgc > 2:1134719/1‑74 (MQ=255) tttCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACcgccgc < 1:1134719/74‑1 (MQ=255) cTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTa > 2:161862/1‑83 (MQ=255) cTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTa < 1:161862/83‑1 (MQ=255) cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg < 1:799874/78‑1 (MQ=255) cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg > 2:799874/1‑78 (MQ=255) cgATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGAt > 2:30737/1‑139 (MQ=255) aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg < 1:1046803/89‑1 (MQ=255) aTTGGAAAATTCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg > 2:1046803/1‑89 (MQ=255) ttGGAAAATTCGCGGGATTTCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCa > 1:794230/1‑139 (MQ=255) tCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg > 2:456070/1‑35 (MQ=255) tCGCGGGATTCCTGCGGACTTAGGAGACCGCcgcg < 1:456070/35‑1 (MQ=255) tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGaa > 2:653226/1‑118 (MQ=255) tCGCGGGATTCCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGaa < 1:653226/118‑1 (MQ=255) cgGGATTTCTGCGGACTTAGGAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAc < 2:794230/139‑1 (MQ=255) | CGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAC > NC_000913/4215607‑4215858 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |