Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,328,224	G→A	E364K (GAA→AAA)	crfC →	clamp‑binding sister replication fork colocalization protein, dynamin‑related

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,328,224	0	G	A	100.0%	199.8 / NA	61	E364K (GAA→AAA)	crfC	clamp‑binding sister replication fork colocalization protein, dynamin‑related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/27);  total (34/27)

CGCTGATGAAAGGCTGTATTACGCCACAGCAGATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGGAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCAATTCAGGCGTT  >  NC_000913/4328090‑4328351                                                                                                                                       |                                                                                                                                cGCTGATGAAAGGCTGTATTACGCCACAGCAGATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGa                                                                                                                             >  1:348114/1‑139 (MQ=255)            ggCTGTATTACGCCACAGCAGATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATg                                                                                                                  <  2:635744/139‑1 (MQ=255)                gTATTACGCCACAGCAGATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGc                                                                                                                       <  2:853948/130‑1 (MQ=255)                gTATTACGCCACAGCAGATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGc                                                                                                                       >  1:853948/1‑130 (MQ=255)                        ccACAGCAGATATTTCCGGTGTCGTCGATGTGAGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCa                                                                                                      >  1:724192/1‑139 (MQ=255)                           cagcagATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATg                                                                                                                  >  2:542218/1‑124 (MQ=255)                           cagcagATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATg                                                                                                                  <  1:542218/124‑1 (MQ=255)                           cagcagATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGgc                                                                                                   >  1:22752/1‑139 (MQ=255)                                          gtgtCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCg                                                                                    >  2:209613/1‑139 (MQ=255)                                               gtcgATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTg                                                                               >  1:864295/1‑139 (MQ=255)                                                tcgATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTgg                                                                              <  1:96862/139‑1 (MQ=255)                                                     gtgGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGAc                                                                         >  2:380781/1‑139 (MQ=255)                                                       ggggCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCt                                                                       >  1:355351/1‑139 (MQ=255)                                                          gCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGa                                                                    <  2:967036/139‑1 (MQ=255)                                                          gCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGa                                                                    <  1:380781/139‑1 (MQ=255)                                                           cTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGTGTTGGCGTCATGCCGATCTGGCGGACCTCGaa                                                                   <  1:474849/139‑1 (MQ=255)                                                             aCCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCAAACa                                                                 >  1:1032575/1‑139 (MQ=255)                                                              ccTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGc                                                                                                                       >  2:1120453/1‑84 (MQ=255)                                                              ccTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGc                                                                                                                       <  1:1120453/84‑1 (MQ=255)                                                              ccTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACat                                                                <  2:724192/139‑1 (MQ=255)                                                              ccTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACat                                                                >  2:1136932/1‑139 (MQ=255)                                                                  gCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACatactg                                                            >  1:298705/1‑136 (MQ=255)                                                                       tCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGTCGATCTGGCGGACCTCGAACATATTCGTCAt                                                       >  2:764073/1‑139 (MQ=255)                                                                       tCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGAcc                                                                        >  1:55477/1‑122 (MQ=255)                                                                       tCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGAcc                                                                        <  2:55477/122‑1 (MQ=255)                                                                       tCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCAt                                                       >  1:468358/1‑139 (MQ=255)                                                                        cGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCAtg                                                      >  2:46174/1‑139 (MQ=255)                                                                        cGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCAAACATATTCGTCAtg                                                      <  2:1032575/139‑1 (MQ=255)                                                                            cgcgcTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACatactgtctcttatac                                                  <  2:298705/139‑14 (MQ=255)                                                                            cgcgcTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCAtgctgc                                                  >  2:412582/1‑139 (MQ=255)                                                                                cTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              <  2:468358/139‑1 (MQ=255)                                                                                cTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              <  1:1014754/139‑1 (MQ=255)                                                                                cTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              <  2:22752/139‑1 (MQ=255)                                                                                cTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              <  1:1136932/139‑1 (MQ=255)                                                                                cTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              <  1:412582/139‑1 (MQ=255)                                                                                cTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              <  2:1091994/139‑1 (MQ=255)                                                                                         aGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTgg                                     >  2:592978/1‑139 (MQ=255)                                                                                           ccAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGa                                   >  2:13780/1‑139 (MQ=255)                                                                                           ccAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGa                                   >  1:581536/1‑139 (MQ=255)                                                                                             aacaacGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAg                                 >  2:807535/1‑139 (MQ=255)                                                                                               caacGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCt                                                                       >  2:253414/1‑99 (MQ=255)                                                                                               caacGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCt                                                                       <  1:253414/99‑1 (MQ=255)                                                                                               caacGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGAt                               >  2:1136584/1‑139 (MQ=255)                                                                                                aacGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGAtt                              <  1:13780/139‑1 (MQ=255)                                                                                                     tAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGc                                                                                                                       >  1:771668/1‑45 (MQ=255)                                                                                                     tAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGc                                                                                                                       <  2:771668/45‑1 (MQ=255)                                                                                                     tAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              >  2:1004134/1‑118 (MQ=255)                                                                                                     tAAGTTACCACCGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGAt                                              <  1:1004134/118‑1 (MQ=255)                                                                                                             caccGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCcgcg                                                                                                             >  2:331806/1‑47 (MQ=255)                                                                                                             caccGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCcgcg                                                                                                             <  1:331806/47‑1 (MQ=255)                                                                                                               ccGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGTCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGc               >  1:663955/1‑139 (MQ=255)                                                                                                               ccGCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGc               <  2:348114/139‑1 (MQ=255)                                                                                                                 gCCAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCa             >  1:433810/1‑139 (MQ=255)                                                                                                                  ccAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCaa            >  1:862961/1‑139 (MQ=255)                                                                                                                  ccAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCaa            >  1:649839/1‑139 (MQ=255)                                                                                                                   cAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGc                    <  1:1163506/130‑1 (MQ=255)                                                                                                                   cAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGc                    >  2:1163506/1‑130 (MQ=255)                                                                                                                   cAGAGCAACAACGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCAAt           >  1:107666/1‑139 (MQ=255)                                                                                                                          acaacGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCAATTCAGGCg    <  1:592978/139‑1 (MQ=255)                                                                                                                          acaacGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCAATTCAGGCg    <  2:864295/139‑1 (MQ=255)                                                                                                                            aacGCTGGGTGAAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCAATTCAGGCGtt  >  1:995036/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                CGCTGATGAAAGGCTGTATTACGCCACAGCAGATATTTCCGGTGTCGTCGATGTGGGGCTACCTGGCGAATCGGGCGCGCTATGAGTTAGCCAACAACGGTAAGTTACCACCGCCAGAGCAACAACGCTGGGTGGAAGATTTTGCCCATGCCGCGCTTGGCAGGCGCTGGCGTCATGCCGATCTGGCGGACCTCGAACATATTCGTCATGCTGCCGATCAGTTGTGGGAAGATTCGCTGTTCGCCCAGCCAATTCAGGCGTT  >  NC_000913/4328090‑4328351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑