Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,373,635	G→C	Y200* (TAC→TAG)	yjeJ ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,373,635	0	G	C	100.0%	187.3 / NA	52	Y200* (TAC→TAG)	yjeJ	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (23/29);  total (23/29)

CGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTG  >  NC_000913/4373500‑4373759                                                                                                                                        |                                                                                                                             cgcgAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTaa                                                                                                                           <  2:1179659/139‑1 (MQ=255)  gcgAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTaaa                                                                                                                          >  2:642273/1‑139 (MQ=255)   cgAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAg                                                                                                                         >  1:29867/1‑139 (MQ=255)     aGTCTTTTCAGTCGTGGGCTGAAATTAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCACTAAATTGTTCTTTGTCACTTTCATCTTTAAAGCGCTAAAGCa                                                                                                                       <  2:787669/139‑1 (MQ=255)         ttttCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGc                                                                                                                   <  1:478423/139‑1 (MQ=255)          tttCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGcc                                                                                                                  >  1:32020/1‑139 (MQ=255)              aGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGAt                                                                                                              <  1:998815/139‑1 (MQ=255)              aGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGAt                                                                                                              <  2:29867/139‑1 (MQ=255)                    gggCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGt                                                                                                        <  1:1060588/139‑1 (MQ=255)                     ggCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAg                                                                                                                         >  1:1210836/1‑121 (MQ=255)                     ggCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAg                                                                                                                         <  2:1210836/121‑1 (MQ=255)                      gCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCg                                                                                                      <  1:642273/139‑1 (MQ=255)                                aGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTAt                                                                                            <  2:33735/139‑1 (MQ=255)                                aGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTAt                                                                                            >  1:162876/1‑139 (MQ=255)                                 gCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATg                                                                                           >  1:319016/1‑139 (MQ=255)                                       ggCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGc                                                                                                                   >  1:103100/1‑109 (MQ=255)                                       ggCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGc                                                                                                                   <  2:103100/109‑1 (MQ=255)                                       agCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCa                                                                                     >  1:761831/2‑139 (MQ=255)                                           aGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTcc                                                                                 >  2:1070015/1‑139 (MQ=255)                                                    ttCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGaa                                                                        <  2:319016/139‑1 (MQ=255)                                                        aTTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAgg                                                                    <  2:761831/139‑1 (MQ=255)                                                          ttCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGgtg                                                                  <  2:1082732/139‑1 (MQ=255)                                                          ttCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGgtg                                                                  <  2:162876/139‑1 (MQ=255)                                                                aCCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCa                                                                                                                       <  2:787023/80‑1 (MQ=255)                                                                aCCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCa                                                                                                                       >  1:787023/1‑80 (MQ=255)                                                                      cGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGaa                                                                        <  1:73652/121‑1 (MQ=255)                                                                      cGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGaa                                                                        >  2:73652/1‑121 (MQ=255)                                                                      cGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGa                                                      >  2:1029076/1‑139 (MQ=255)                                                                       gTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTc                                                                <  2:693774/128‑1 (MQ=255)                                                                       gTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTc                                                                >  1:693774/1‑128 (MQ=255)                                                                            ttCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCa                                                <  1:497837/139‑1 (MQ=255)                                                                                cgcgTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGaa                                                                        <  2:447164/111‑1 (MQ=255)                                                                                cgcgTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGaa                                                                        >  1:447164/1‑111 (MQ=255)                                                                                cgcgTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGt                                            >  2:300475/1‑139 (MQ=255)                                                                                     tttCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTgg                                       <  2:713936/139‑1 (MQ=255)                                                                                               gcgcCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGtt                                                                             <  2:956474/91‑1 (MQ=255)                                                                                               gcgcCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGtt                                                                             >  1:956474/1‑91 (MQ=255)                                                                                                cgccgcTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAcg                              >  2:981355/1‑137 (MQ=255)                                                                                                cgccgcTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAAcg                              <  1:981355/137‑1 (MQ=255)                                                                                                     cTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAg                                                                                                >  2:482640/1‑66 (MQ=255)                                                                                                     cTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAg                                                                                                <  1:482640/66‑1 (MQ=255)                                                                                                     cTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGa                                                      <  1:311180/108‑1 (MQ=255)                                                                                                     cTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGa                                                      >  2:311180/1‑108 (MQ=255)                                                                                                           tGTTCTTTGCCACTTTCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCa                 <  1:1070015/139‑1 (MQ=255)                                                                                                                          tCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTg  >  2:711695/1‑139 (MQ=255)                                                                                                                          tCATCTTTAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTg  <  2:211955/139‑1 (MQ=255)                                                                                                                                 tAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCAt                                               <  2:897738/87‑1 (MQ=255)                                                                                                                                 tAAAGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCAt                                               >  1:897738/1‑87 (MQ=255)                                                                                                                                  aaaGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAAt                                                                       <  1:302690/62‑1 (MQ=255)                                                                                                                                  aaaGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAAt                                                                       >  2:302690/1‑62 (MQ=255)                                                                                                                                  aaaGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCaa                                >  1:975536/1‑101 (MQ=255)                                                                                                                                  aaaGCGCTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCaa                                <  2:975536/101‑1 (MQ=255)                                                                                                                                        |                                                                                                                             CGCGAGTTTTTTCAGTCGTGGGCTGAAATCAAGCATTCGGCGAGTGATCGCTTCTATTTCTTTACCCGGCGTTGCTTCACGCGTTTTCACCACTGCGCCGCTAAATTGTTCTTTGCCACTTTCATCTTTAAAGCGGTAAAGCACCGCCAGATAATGGTCGAACAAGTTATGTTTCCATTCCGGTTGGGAATAGGTGTCATATTCCAGATTGCCATTGTCCTGGCAGTCAACGTCGTATAACGGCAGGAAATCTAACAGTG  >  NC_000913/4373500‑4373759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑