Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,422,793	C→T	A203V (GCC→GTC)	ulaD →	3‑keto‑L‑gulonate 6‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,422,793	0	C	T	100.0%	112.2 / NA	36	A203V (GCC→GTC)	ulaD	3‑keto‑L‑gulonate 6‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/17);  total (19/17)

TGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCA  >  NC_000913/4422670‑4422922                                                                                                                            |                                                                                                                                  tGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCaa                                                                                                                    >  2:1138173/1‑139 (MQ=255)        cAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTcc                                                                                                             >  1:667697/1‑139 (MQ=255)             tcaccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                            <  2:476522/135‑1 (MQ=255)             tcaccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCa                                                                                                            >  1:476522/1‑135 (MQ=255)               accgtcaccgGAGGCCTGGTGCTGGAAGATCTGCCGCTGTTCAAGGGTATTTCGATTCACGTCGTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCTAACGTTCCATCGCTg                                                                                                      <  1:813378/139‑1 (MQ=255)                ccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGa                                                                                                     >  2:964604/1‑139 (MQ=255)                              cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                       <  2:419401/139‑1 (MQ=255)                               tGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                      <  1:1138173/139‑1 (MQ=255)                                 gcgcTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAg                                                                                    >  1:696102/1‑139 (MQ=255)                                               gCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                              >  2:584093/1‑99 (MQ=255)                                               gCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTc                                                                                                              <  1:584093/99‑1 (MQ=255)                                                 cGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCcaa                                                                    >  1:764991/1‑139 (MQ=255)                                                      ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTg                                                                                                      <  2:1008465/100‑1 (MQ=255)                                                      ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTg                                                                                                      >  1:1008465/1‑100 (MQ=255)                                                                ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCg                                                                                  >  1:1123096/1‑110 (MQ=255)                                                                ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCg                                                                                  <  2:1123096/110‑1 (MQ=255)                                                                ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTgg                                                     >  2:151991/1‑139 (MQ=255)                                                                        cACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                       <  1:298224/97‑1 (MQ=255)                                                                        cACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                       >  2:298224/1‑97 (MQ=255)                                                                                       ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAg                                                                                                                         >  1:781130/1‑48 (MQ=255)                                                                                       ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAg                                                                                                                         <  2:781130/48‑1 (MQ=255)                                                                                                aTCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGt                     <  2:667697/139‑1 (MQ=255)                                                                                                  ccGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTc                                                                                                                      >  1:503374/1‑40 (MQ=255)                                                                                                  ccGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTc                                                                                                                      <  2:503374/40‑1 (MQ=255)                                                                                                      gATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCg                                                                                                         <  1:553925/49‑1 (MQ=255)                                                                                                      gATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCg                                                                                                         >  2:553925/1‑49 (MQ=255)                                                                                                      gATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggc               >  1:1119125/1‑139 (MQ=255)                                                                                                      gATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggc               >  1:604175/1‑139 (MQ=255)                                                                                                         gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtgtg                                                                                               <  1:85580/56‑1 (MQ=255)                                                                                                         gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACtgtg                                                                                               >  2:85580/1‑56 (MQ=255)                                                                                                         gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCg                                                          <  1:542510/93‑1 (MQ=255)                                                                                                         gCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCg                                                          >  2:542510/1‑93 (MQ=255)                                                                                                          ccGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa           <  2:764991/139‑1 (MQ=255)                                                                                                                   cGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCa  <  2:696102/139‑1 (MQ=255)                                                                                                                        gAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg            >  2:110378/1‑124 (MQ=255)                                                                                                                        gAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctgg            <  1:110378/124‑1 (MQ=255)                                                                                                                            |                                                                                                                                  TGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCA  >  NC_000913/4422670‑4422922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑