Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,577,958	C→T	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC ← mcrB ←	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,577,958	0	C	T	100.0%	278.5 / NA	85	M1M (GTG→ATG) † *460* (TAG→TAA)	mcrC mcrB	5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrC 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/38);  total (47/38)

ACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577829‑4578091                                                                                                                                  |                                                                                                                                      acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                   <  2:238741/134‑1 (MQ=255) acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATg                                                                                                                                   >  1:238741/1‑134 (MQ=255) acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  2:84691/1‑139 (MQ=255) acCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTcc                                                                                                                              >  2:1058286/1‑139 (MQ=255)      aGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaa                                                                                                                         >  1:530684/1‑139 (MQ=255)       ggATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaat                                                                                                                        >  1:387089/1‑139 (MQ=255)        gATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTAt                                                                                                                                    <  2:918914/126‑1 (MQ=255)        gATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTAt                                                                                                                                    >  1:918914/1‑126 (MQ=255)        gATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCtaata                                                                                                                       >  2:313209/1‑139 (MQ=255)        gATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCta                                                                                                                          >  1:615557/1‑136 (MQ=255)        gATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCta                                                                                                                          <  2:615557/136‑1 (MQ=255)             cAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttg                                                                                                                  >  2:584154/1‑139 (MQ=255)             cAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttg                                                                                                                  >  1:116519/1‑139 (MQ=255)             cAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttg                                                                                                                  >  1:907901/1‑139 (MQ=255)              aagaagATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATttgt                                                                                                                 <  2:116519/139‑1 (MQ=255)                      tGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAt                                                                                                         <  1:202465/139‑1 (MQ=255)                        ttACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAttt                                                                                                       <  2:387089/139‑1 (MQ=255)                          aCCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATGTTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     >  2:734726/1‑139 (MQ=255)                          aCCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTAt                                                                                                                                    <  1:497353/108‑1 (MQ=255)                          aCCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTAt                                                                                                                                    >  2:497353/1‑108 (MQ=255)                             gggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  1:313209/139‑1 (MQ=255)                             gggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgtt                                                                                                  <  2:530684/139‑1 (MQ=255)                              ggTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCtgttg                                                                                                 >  2:1010524/1‑139 (MQ=255)                                tATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTgttgtt                                                                                               >  1:1004835/1‑139 (MQ=255)                                tATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTgttgtt                                                                                               >  2:12579/1‑139 (MQ=255)                                  tGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTta                                                                                             <  1:584154/139‑1 (MQ=255)                                  tGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAA‑CCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTtat                                                                                            >  1:764811/1‑139 (MQ=255)                                   ggCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTtat                                                                                            >  1:939295/1‑139 (MQ=255)                                         aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTccc                                                                                                                             >  2:633366/1‑100 (MQ=255)                                         aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTccc                                                                                                                             <  1:633366/100‑1 (MQ=255)                                         aaGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGt                                                                                      >  2:406611/1‑139 (MQ=255)                                              ttGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 <  1:12579/139‑1 (MQ=255)                                                               tGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  2:1175840/1‑74 (MQ=255)                                                               tGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  1:1175840/74‑1 (MQ=255)                                                                   aaTAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTaaa                                                            <  2:1004835/139‑1 (MQ=255)                                                                          ccATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGa                                                     <  1:1058286/139‑1 (MQ=255)                                                                             tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            <  2:336197/81‑1 (MQ=255)                                                                             tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTc                                                                                                            >  1:336197/1‑81 (MQ=255)                                                                             tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   >  1:305906/1‑106 (MQ=255)                                                                             tGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   <  2:305906/106‑1 (MQ=255)                                                                                aTAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCg                                               <  2:939295/139‑1 (MQ=255)                                                                                   ggTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   <  1:1205960/100‑1 (MQ=255)                                                                                   ggTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcat                                                                                   >  2:1205960/1‑100 (MQ=255)                                                                                       aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtc                                                                                     >  2:616891/1‑94 (MQ=255)                                                                                       aGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtc                                                                                     <  1:616891/94‑1 (MQ=255)                                                                                         cATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATAAGTCCCGTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACaa                                      >  1:704772/1‑139 (MQ=255)                                                                                           tGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  2:649364/1‑46 (MQ=255)                                                                                           tGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  1:649364/46‑1 (MQ=255)                                                                                            gTAATAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAAttt                                   >  2:182977/1‑139 (MQ=255)                                                                                              aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 <  2:94401/43‑1 (MQ=255)                                                                                              aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAg                                                                                                                                 >  1:94401/1‑43 (MQ=255)                                                                                              aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 >  1:155097/1‑91 (MQ=255)                                                                                              aaTAGATATTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 <  2:155097/91‑1 (MQ=255)                                                                                                   atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAg                            >  2:716637/1‑139 (MQ=255)                                                                                                   atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAg                            >  1:674629/1‑139 (MQ=255)                                                                                                   atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAg                            >  2:1076009/1‑139 (MQ=255)                                                                                                   atatTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAg                            >  1:123335/1‑139 (MQ=255)                                                                                                     atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     <  1:442239/64‑1 (MQ=255)                                                                                                     atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCt                                                                                                     >  2:442239/1‑64 (MQ=255)                                                                                                     atTACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGcc                          <  2:926484/139‑1 (MQ=255)                                                                                                      ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCa                         <  1:527669/139‑1 (MQ=255)                                                                                                      ttACGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCa                         >  2:527669/1‑139 (MQ=255)                                                                                                         cGGACAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTg                      <  2:907901/139‑1 (MQ=255)                                                                                                            aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                        >  1:60106/1‑102 (MQ=255)                                                                                                            aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAgggg                                                        <  2:60106/102‑1 (MQ=255)                                                                                                            aCAGGTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGt                   >  1:212916/1‑139 (MQ=255)                                                                                                                gTATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATcc               <  1:406611/139‑1 (MQ=255)                                                                                                                 tATCACGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCg              <  2:704772/139‑1 (MQ=255)                                                                                                                      cGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGagag         <  1:1076009/139‑1 (MQ=255)                                                                                                                      cGGGCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGagag         >  1:1165678/1‑139 (MQ=255)                                                                                                                        ggCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatc                                                                                  <  1:273921/64‑1 (MQ=255)                                                                                                                        ggCTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatc                                                                                  >  2:273921/1‑64 (MQ=255)                                                                                                                          cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 >  1:282562/1‑63 (MQ=255)                                                                                                                          cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGtcatca                                                                                 <  2:282562/63‑1 (MQ=255)                                                                                                                          cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGc                     >  1:75085/1‑123 (MQ=255)                                                                                                                          cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGc                     <  2:75085/123‑1 (MQ=255)                                                                                                                          cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGc     >  1:352377/1‑139 (MQ=255)                                                                                                                          cTGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGc     >  2:1194536/1‑139 (MQ=255)                                                                                                                           tGTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGcc    >  1:1109915/1‑139 (MQ=255)                                                                                                                            gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCa   <  2:1084079/139‑1 (MQ=255)                                                                                                                            gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCa   <  1:84691/139‑1 (MQ=255)                                                                                                                            gTTCCATTATGAGTCCCCTAATAATTTGTTGGTCCAATTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCa   <  2:764811/139‑1 (MQ=255)                                                                                                                             ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAt  >  2:859906/1‑139 (MQ=255)                                                                                                                             ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCAATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTg      <  2:635964/135‑1 (MQ=255)                                                                                                                             ttCCATTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCAATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTg      >  1:635964/1‑135 (MQ=255)                                                                                                                                  |                                                                                                                                      ACCCCAGGATATCAAGAAGATTGTTACCGGGTATGGCTTCAAGGTTTGCCTGCTTAATTTCCTGTAAATAACCCCATGCATAGGTAAGCATGTAATAGATATTACGGACAGGTATCACGGGCTGTTCCACTATGAGTCCCCTAATAATTTGTTGGTCCATTTCTGTTGTTTATAGGGGTCATCAAAGAAATATTCTTCGAGTAAAGGGGCGATATCCGTCATCACAATTTCATTAAGCCATTGCGTATCCGGAGAGGTGCCAT  >  NC_000913/4577829‑4578091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑