Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,532	T→C	D260G (GAT→GGT)	rob ←	right oriC‑binding transcriptional activator, AraC family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,532	0	T	C	100.0%	160.7 / NA	46	D260G (GAT→GGT)	rob	right oriC‑binding transcriptional activator, AraC family
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/22);  total (24/22)

ATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGAT  >  NC_000913/4634407‑4634667                                                                                                                              |                                                                                                                                        aTCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATTAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCTTGACCTTTACg                                                                                                                            >  1:32177/1‑139 (MQ=255)  tCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACgg                                                                                                                           <  1:1012403/139‑1 (MQ=255)           cccTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAgg                                                                                                                  >  1:477695/1‑139 (MQ=255)             cTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGtt                                                                                                                >  2:572248/1‑139 (MQ=255)              tGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTg                                                                                                               >  2:110759/1‑139 (MQ=255)                        gAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCa                                                                                                     <  2:249980/139‑1 (MQ=255)                        gAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCa                                                                                                     <  1:195758/139‑1 (MQ=255)                          gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTc                                                                                                                     <  2:747873/121‑1 (MQ=255)                          gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTc                                                                                                                     >  1:747873/1‑121 (MQ=255)                          gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                                                   >  2:739286/1‑139 (MQ=255)                          gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                                                   >  2:290896/1‑139 (MQ=255)                          gCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCACTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATg                                                                                                   >  2:657972/1‑139 (MQ=255)                             gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                                >  2:808726/1‑139 (MQ=255)                             gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                                <  1:450864/139‑1 (MQ=255)                             gCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCACTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCAc                                                                                                <  1:657972/139‑1 (MQ=255)                              cAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACg                                                                                               >  1:517796/1‑139 (MQ=255)                                            gATCTGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGc                                                                                                  <  2:221838/122‑1 (MQ=255)                                            gATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGc                                                                                                  >  1:221838/1‑122 (MQ=255)                                             aTCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCa                                                                                >  1:637811/1‑139 (MQ=255)                                                           ttCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTGACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGc                                                                  >  1:770609/1‑139 (MQ=255)                                                                aGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                                             <  2:477695/139‑1 (MQ=255)                                                                     aGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTc                                                        >  1:1179248/1‑139 (MQ=255)                                                                      gATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTcc                                                       <  2:517796/139‑1 (MQ=255)                                                                       aTTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTccc                                                      >  1:413358/1‑139 (MQ=255)                                                                            ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                 <  2:637811/139‑1 (MQ=255)                                                                            ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                 >  2:1039414/1‑139 (MQ=255)                                                                            ttGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGAcc                                                 >  1:117765/1‑139 (MQ=255)                                                                              gggCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCtt                                               <  2:413358/139‑1 (MQ=255)                                                                                 cGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCAt                                            >  2:546071/1‑139 (MQ=255)                                                                                 cGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCAt                                            >  2:999865/1‑139 (MQ=255)                                                                                     ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:999865/139‑1 (MQ=255)                                                                                     ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:572248/139‑1 (MQ=255)                                                                                     ctcCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:290896/139‑1 (MQ=255)                                                                                     ctcCCGCTTTGGCATCTTCGGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGt                                        <  1:92083/139‑1 (MQ=255)                                                                                     ctaaggatatGGCATATTCTGCCGGGTAGTCCCGCCCAATCCCCGGAACATTCCGGCGCGTCAGGTTGAGCATTGGCATGCACGATCCCTATACCGTCAGGATATACTCCTGCACGCCGGTTCCCAGACCCTCATAGGt                                        <  1:608331/130‑1 (MQ=255)                                                                                        ccGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTaaa                                     <  1:110759/139‑1 (MQ=255)                                                                                           cTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGtt                                                                                             <  2:1045285/80‑1 (MQ=255)                                                                                           cTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGtt                                                                                             >  1:1045285/1‑80 (MQ=255)                                                                                               ggCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                                             <  2:1050916/108‑1 (MQ=255)                                                                                               ggCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGcc                                                             >  1:1050916/1‑108 (MQ=255)                                                                                                gCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCtt                                               <  2:517699/121‑1 (MQ=255)                                                                                                gCATCTTCTGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCtt                                               >  1:517699/1‑121 (MQ=255)                                                                                                     ttctGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAg                                                    <  2:971498/111‑1 (MQ=255)                                                                                                     ttctGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAg                                                    >  1:971498/1‑111 (MQ=255)                                                                                                      tctGCCGGGTAGTATCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTcgc                       <  2:770609/139‑1 (MQ=255)                                                                                                                 gtaTCGCTCAATACCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTgcagc            >  2:710623/1‑139 (MQ=255)                                                                                                                           atacCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGAt  >  1:1019445/1‑139 (MQ=255)                                                                                                                              |                                                                                                                                        ATCTGGACGCCCCTGCATTAGATGAGCTGCAGCGTTAACGACGGATCGGAATCAGCAGTTCACAGCGTAGATTAATTGGGCGATCTCCCGCTTTGGCATCTTCTGCCGGGTAGTATCGCTCAATATCCTGACCTTTACGGCGCGTCAGGTTGAGCATTGGCATGCACGTTCCGTATACCGTCAGGATAAACTCCTGCACGCCGGTTCCCAGACCTTCATAGGTAAACATCACATATTCGCCGCCCTGCAGCATCACCGGAT  >  NC_000913/4634407‑4634667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑