Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	411,297	C→T	*395* (TAG→TAA)	araJ ←	L‑arabinose‑inducible putative transporter, MFS family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	411,297	0	C	T	100.0%	114.1 / NA	36	*395* (TAG→TAA)	araJ	L‑arabinose‑inducible putative transporter, MFS family
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/16);  total (20/16)

GGCAATAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGC  >  NC_000913/411162‑411425                                                                                                                                        |                                                                                                                                 ggCAATAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTAc                                                                                                                               <  1:360455/139‑1 (MQ=255)            cTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAAT‑AGTAATGAACAACGAGACTATAACTTACCCCaagtgtttt                                                                                                                   >  2:27069/1‑131 (MQ=21)               cGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGc                                                                                                                 <  2:718143/139‑1 (MQ=255)               cGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGc                                                                                                                 <  1:874930/139‑1 (MQ=255)                 aTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCa                                                                                                               >  1:694146/1‑139 (MQ=255)                  tGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAg                                                                                                              >  2:346/1‑139 (MQ=255)                  tGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAg                                                                                                              <  1:346/139‑1 (MQ=255)                                                   ccTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGc                                                                                                                 >  2:227603/1‑103 (MQ=255)                                                   ccTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGc                                                                                                                 <  1:227603/103‑1 (MQ=255)                                                     tGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                           >  1:467689/1‑139 (MQ=255)                                                     tGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                           >  1:606512/1‑139 (MQ=255)                                                       cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAg                                                                                                              >  2:17339/1‑102 (MQ=255)                                                       cAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAg                                                                                                              <  1:17339/102‑1 (MQ=255)                                                        aaaaTGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGAc                                                                        <  2:694146/139‑1 (MQ=255)                                                                 cAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATAcagca                                                               >  1:887148/1‑139 (MQ=255)                                                                                   aTAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCa                                                                                                                           <  1:90977/61‑1 (MQ=255)                                                                                   aTAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCa                                                                                                                           >  2:90977/1‑61 (MQ=255)                                                                                        gCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCaaat                                        >  2:884826/1‑138 (MQ=255)                                                                                             cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGt                                                                                                                      <  2:105739/56‑1 (MQ=255)                                                                                             cACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGt                                                                                                                      >  1:105739/1‑56 (MQ=255)                                                                                                  cgcATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                           >  1:1168296/1‑94 (MQ=255)                                                                                                  cgcATCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGc                                                                           <  2:1168296/94‑1 (MQ=255)                                                                                                      tCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAgg                               >  1:1121655/1‑134 (MQ=255)                                                                                                      tCCGGCAATAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAgg                               <  2:1121655/134‑1 (MQ=255)                                                                                                              tAAGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAgcg                  >  1:208873/1‑139 (MQ=255)                                                                                                                aGTAATGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGcc                <  2:348139/139‑1 (MQ=255)                                                                                                                    aTGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGt            <  2:606512/139‑1 (MQ=255)                                                                                                                     tGAGCACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTa           >  2:694992/1‑139 (MQ=255)                                                                                                                         cACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTgg                                                                                     <  2:498936/61‑1 (MQ=255)                                                                                                                         cACCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTgg                                                                                     >  1:498936/1‑61 (MQ=255)                                                                                                                          aCCGAGACTATAACTTATCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATtat      >  2:762075/1‑139 (MQ=255)                                                                                                                          aCCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCAc              >  2:1158798/1‑131 (MQ=255)                                                                                                                          aCCGAGACTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCAc              <  1:1158798/131‑1 (MQ=255)                                                                                                                              agaCTATAACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGc  >  2:999627/1‑139 (MQ=255)                                                                                                                                   ataACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATa                                                                    <  2:139184/68‑1 (MQ=255)                                                                                                                                   ataACTTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATa                                                                    >  1:139184/1‑68 (MQ=255)                                                                                                                                        |                                                                                                                                 GGCAATAAGTGCTCCGATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGCAAAATGTGCCAGAACCGCGTAGGGCGGATAAGGCGTTCACGCCGCATCCGGCAATAAGTAATGAGCACCGAGACTATAACCTACCCCAGTGGTTTCGCCAGCACCGGAGTATCCGCCGCTTGCTGGCGCTTATAGCGACCATACAGCAGCAACGACGACATCGCAGCAAACGAAAGCAGGGCGGCAGGCAGCGCCACGTAATTATATGC  >  NC_000913/411162‑411425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑