Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	437,107	G→A	*173* (TAG→TAA)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	437,107	0	G	A	100.0%	152.0 / NA	48	*173* (TAG→TAA)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

GATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCA  >  NC_000913/436978‑437240                                                                                                                                  |                                                                                                                                      gATCCGCTGGTTTGATCGCAATGTGCATGGCGACATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCAcc                                                                                                                              <  1:1001793/139‑1 (MQ=255)       cTGGTTTGATCGCAATGTGCATGGCAGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGcc                                                                                                                        <  2:1106844/139‑1 (MQ=255)                           aTGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCTTGATGTGACGCTTGTCACGt                                                                                                    >  2:822272/1‑139 (MQ=255)                           aTGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGt                                                                                                    <  2:938015/139‑1 (MQ=255)                                ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGCTTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTGATTGTTCACCGATGCCTGAtgt                                                                                                                  >  1:595931/1‑120 (MQ=255)                                ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGAtgt                                                                                                                  <  2:595931/120‑1 (MQ=255)                                  cATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               <  2:178780/137‑1 (MQ=255)                                  cATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               >  1:178780/1‑137 (MQ=255)                                      gggATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGcc                                                                                         >  1:187478/1‑139 (MQ=255)                                        gATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTg                                                                                       <  2:671782/139‑1 (MQ=255)                                         aTCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTgg                                                                                      >  2:229733/1‑139 (MQ=255)                                                 cgacgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTAt                                                                              >  2:1129162/1‑139 (MQ=255)                                                   acgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               <  1:406288/120‑1 (MQ=255)                                                   acgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               >  2:406288/1‑120 (MQ=255)                                                   acgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGTCTGGACTCTTAttt                                                                            <  2:187478/139‑1 (MQ=255)                                                   acgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTAttt                                                                            >  1:1072290/1‑139 (MQ=255)                                                    cgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                           <  1:173209/139‑1 (MQ=255)                                                                 ggggTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAg                                                              >  2:350920/1‑139 (MQ=255)                                                                 ggggTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAg                                                              >  1:916528/1‑139 (MQ=255)                                                                             gCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTtat                                                  <  1:822272/139‑1 (MQ=255)                                                                                          aTTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAg                                     <  1:350920/139‑1 (MQ=255)                                                                                              tATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCg                                 >  2:778147/1‑139 (MQ=255)                                                                                               aTCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCgg                                <  1:1129162/139‑1 (MQ=255)                                                                                                tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               <  2:1050787/75‑1 (MQ=255)                                                                                                tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               >  1:1050787/1‑75 (MQ=255)                                                                                                 cGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCa                              >  1:749782/1‑139 (MQ=255)                                                                                                   gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAAt                            <  2:665350/139‑1 (MQ=255)                                                                                                   gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAAt                            >  1:749290/1‑139 (MQ=255)                                                                                                   gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAAt                            >  1:665350/1‑139 (MQ=255)                                                                                                   gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAAt                            >  2:851531/1‑139 (MQ=255)                                                                                                   gTCATCAATGGCCGCTGGGTATTCAGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAAt                            >  2:743255/1‑139 (MQ=255)                                                                                                     catcaCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGACGTTTCCAGTTTGGCAATCt                          >  1:1011806/1‑139 (MQ=255)                                                                                                      atcaCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTg                         >  2:1083086/1‑139 (MQ=255)                                                                                                         aCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTc                      <  1:1083086/139‑1 (MQ=255)                                                                                                                 cTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGtt                                          <  2:970176/111‑1 (MQ=255)                                                                                                                 cTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGtt                                          >  1:970176/1‑111 (MQ=255)                                                                                                                 cTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCa              >  2:668297/1‑139 (MQ=255)                                                                                                                 cTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCa              <  1:668297/139‑1 (MQ=255)                                                                                                                 cTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCa              <  2:916528/139‑1 (MQ=255)                                                                                                                  tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                           <  2:19164/77‑1 (MQ=255)                                                                                                                  tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                           >  1:19164/1‑77 (MQ=255)                                                                                                                  tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTAGACTCTTATTTAAATc                                                                       <  1:958534/81‑1 (MQ=255)                                                                                                                  tGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTAGACTCTTATTTAAATc                                                                       >  2:958534/1‑81 (MQ=255)                                                                                                                             tCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCa  <  2:1072290/139‑1 (MQ=255)                                                                                                                              cGTAATTGTGCACCGATGCCTGATGTGACGCTTGt                                                                                                         <  2:355038/35‑1 (MQ=255)                                                                                                                              cGTAATTGTGCACCGATGCCTGATGTGACGCTTGt                                                                                                         >  1:355038/1‑35 (MQ=255)                                                                                                                              cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               <  2:234883/45‑1 (MQ=255)                                                                                                                              cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                               >  1:234883/1‑45 (MQ=255)                                                                                                                                  |                                                                                                                                      GATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCA  >  NC_000913/436978‑437240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑