Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 443,051 C→T *197* (TAG→TAA)  yajL ← oxidative‑stress‑resistance chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913443,0510CT100.0% 134.1 / NA 42*197* (TAG→TAA) yajLoxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/20);  total (22/20)

AGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGG  >  NC_000913/442918‑443177
                                                                                                                                     |                                                                                                                               
aGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTc                                                                                                                            >  1:429983/1‑139 (MQ=255)
    cgGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGATGTTTGCCGCATCCGACATTACTCGtaa                                                                                                                        <  2:987928/139‑1 (MQ=255)
                   tCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCt                                                                                                         >  1:137970/1‑139 (MQ=255)
                          gcgcTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCa                                                                                                  >  1:39029/1‑139 (MQ=255)
                                      cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTg                                                                                                        <  1:94576/121‑1 (MQ=255)
                                      cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTg                                                                                                        >  2:94576/1‑121 (MQ=255)
                                                    tAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                      >  1:1137167/1‑93 (MQ=255)
                                                    tAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                      <  2:1137167/93‑1 (MQ=255)
                                                       cAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑Ct                                                                    >  2:394337/1‑139 (MQ=255)
                                                           gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                   <  1:1088879/105‑1 (MQ=255)
                                                           gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                   >  2:1088879/1‑105 (MQ=255)
                                                           gtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑Cttttt                                                                >  1:1042222/1‑139 (MQ=255)
                                                              aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccg                                                                                                     >  2:997290/1‑100 (MQ=255)
                                                              aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccg                                                                                                     <  1:997290/100‑1 (MQ=255)
                                                                ggattatCGCCGGTATTTTTTGGTTTTT‑CGTCCGCTCCTGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGcc                                                          <  2:216869/135‑1 (MQ=255)
                                                                   atatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCcca                                                        <  2:137970/139‑1 (MQ=255)
                                                                    tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                      >  1:1001466/1‑77 (MQ=255)
                                                                    tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                      <  2:1001466/77‑1 (MQ=255)
                                                                      tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCAt                                                                                                 >  1:1010876/1‑96 (MQ=255)
                                                                      tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCAt                                                                                                 <  2:1010876/96‑1 (MQ=255)
                                                                        gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCccaaccaa                                                   >  1:282650/1‑139 (MQ=255)
                                                                        gCCCGTAATTTGTGGTTTTTACGTCGCATCT‑GTCAGAATGGGCGTTTGCCGCATTCCACCTTACTCGTAATAATTATAAATTCCTGCCGCCCTCACCCGTTGTGGTGCCCCCTCATGGGCCTTTTTACCGcccacccac                                                   >  1:216869/1‑138 (MQ=255)
                                                                         cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                             <  1:573652/65‑1 (MQ=255)
                                                                         cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                             >  2:573652/1‑65 (MQ=255)
                                                                            gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGcc                                                          >  1:344834/1‑128 (MQ=255)
                                                                            gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGcc                                                          <  2:344834/128‑1 (MQ=255)
                                                                            gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCccaaccaa                                                   >  1:240933/1‑135 (MQ=255)
                                                                            gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCccaaccaa                                                   <  2:240933/135‑1 (MQ=255)
                                                                                        ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTc                                   >  2:37784/1‑139 (MQ=255)
                                                                                              gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCTCCATCACCAGTTGTGATGCCACTTCATGGG‑Cttttt                                                                >  1:459943/1‑104 (MQ=255)
                                                                                              gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCTCCATCACCAGTTGTGATGCCACTTCATGGG‑Cttttt                                                                <  2:459943/104‑1 (MQ=255)
                                                                                              gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                             <  1:37784/139‑1 (MQ=255)
                                                                                              gTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCa                             <  2:429983/139‑1 (MQ=255)
                                                                                                      tGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGAt                     <  2:282650/139‑1 (MQ=255)
                                                                                                        gTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgcc                                                                                                      <  1:288100/57‑1 (MQ=255)
                                                                                                        gTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgcc                                                                                                      >  2:288100/1‑57 (MQ=255)
                                                                                                            gATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                   >  1:520591/1‑56 (MQ=255)
                                                                                                            gATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccgcc                                                                                                   <  2:520591/56‑1 (MQ=255)
                                                                                                               gCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc            <  1:394337/139‑1 (MQ=255)
                                                                                                               gCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc            >  2:517948/1‑139 (MQ=255)
                                                                                                                  aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCAc                                                             >  2:736098/1‑87 (MQ=255)
                                                                                                                  aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCAc                                                             <  1:736098/87‑1 (MQ=255)
                                                                                                                  aCGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg         >  1:947401/1‑139 (MQ=255)
                                                                                                                         ccGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctgg  <  2:1042222/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                               
AGCGCGGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGG‑CTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGG  >  NC_000913/442918‑443177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑