Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 477,025 G→A *118* (TAG→TAA)  ybaA → DUF1428 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913477,0250GA100.0% 151.8 / NA 47*118* (TAG→TAA) ybaADUF1428 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

CTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGC  >  NC_000913/476892‑477158
                                                                                                                                     |                                                                                                                                     
cTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGc                                                                                                                                  <  1:615825/139‑1 (MQ=255)
   cAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTg                                                                                                                               >  2:30086/1‑139 (MQ=255)
   cAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTg                                                                                                                               >  2:102341/1‑139 (MQ=255)
    aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGa                                                                                                                              >  2:1068959/1‑139 (MQ=255)
    aaaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGa                                                                                                                              >  1:159659/1‑139 (MQ=255)
     aaaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGAc                                                                                                                             <  2:506252/139‑1 (MQ=255)
      aaGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACg                                                                                                                            <  2:390568/139‑1 (MQ=255)
        gagGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACggg                                                                                                                          <  2:159659/139‑1 (MQ=255)
             ccGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCtgtg                                                                                                                     <  1:1068959/139‑1 (MQ=255)
              cgcgACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgc                                                                                                                    >  1:853574/1‑139 (MQ=255)
                      tgctAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGcc                                                                                                            >  2:762232/1‑139 (MQ=255)
                        ctAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGt                                                                                                          <  1:762232/139‑1 (MQ=255)
                                aaGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaa                                                                                                  >  2:751112/1‑139 (MQ=255)
                                   atgatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCaaaaa                                                                                               <  1:107953/139‑1 (MQ=255)
                                     gatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAAtt                                                                                             >  2:520229/1‑139 (MQ=255)
                                     gatgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAAtt                                                                                             >  2:1216731/1‑139 (MQ=255)
                                       tgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGc                                                                                                                                  >  1:330084/1‑100 (MQ=255)
                                       tgTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGc                                                                                                                                  <  2:330084/100‑1 (MQ=255)
                                             aCCCACGGGTGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAATATGCACAAATg                                                                                     <  1:578231/139‑1 (MQ=255)
                                                 aCGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACaa                                                                                        <  1:947737/132‑1 (MQ=255)
                                                 aCGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACaa                                                                                        >  2:947737/1‑132 (MQ=255)
                                                  cGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCt                                                                                <  1:520229/139‑1 (MQ=255)
                                                     aTGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCgg                                                                             <  2:454503/139‑1 (MQ=255)
                                                       gAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                           <  1:30086/139‑1 (MQ=255)
                                                              ttCGGCGAGTCCATGCCGTTTGGCGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgc                                                                                                                    <  2:383629/91‑1 (MQ=255)
                                                              ttCGGCGAGTCCATGCCGTTTGGCGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTgc                                                                                                                    >  1:383629/1‑91 (MQ=255)
                                                                                tttGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTa                                                  >  2:537277/1‑139 (MQ=255)
                                                                                                     tATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATg                                                                                     >  1:245624/1‑83 (MQ=255)
                                                                                                     tATGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATg                                                                                     <  2:245624/83‑1 (MQ=255)
                                                                                                       tGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                           <  1:882443/91‑1 (MQ=255)
                                                                                                       tGGCGGATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGcc                                                                           >  2:882443/1‑91 (MQ=255)
                                                                                                            gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTaa                                                 >  1:636626/1‑112 (MQ=255)
                                                                                                            gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTaa                                                 <  2:636626/112‑1 (MQ=255)
                                                                                                            gATTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGAcc                      >  1:64729/1‑139 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAg                                                                        >  2:132806/1‑88 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAg                                                                        <  1:132806/88‑1 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAt                                                              <  2:255824/98‑1 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAt                                                              >  1:255824/1‑98 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAt                                                              >  2:784219/1‑98 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAAt                                                              <  1:784219/98‑1 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                     >  1:1014547/1‑139 (MQ=255)
                                                                                                             aTTCGAGTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCg                     <  1:102341/139‑1 (MQ=255)
                                                                                                                   gTCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGc               >  1:182506/1‑139 (MQ=255)
                                                                                                                    tCAATCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCa              <  1:537277/139‑1 (MQ=255)
                                                                                                                      aaTCATCGACGAATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACg            <  1:751112/139‑1 (MQ=255)
                                                                                                                              acgaATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTg    <  2:64729/139‑1 (MQ=255)
                                                                                                                                gaATAAGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGc  <  2:876677/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                     
CTTCAAAAGAGGTCCGCGACGCTGCTAATCAAAAGATGATGTCGGACCCACGGATGAAAGAGTTCGGCGAGTCCATGCCGTTTGACGGCAAGCGAATGATCTATGGCGGATTCGAGTCAATCATCGACGAATAGGTAGCGTGACGGGCTGTGCGGCGTGCCGTCAGCCCAAAAATTCACAAATGTTGCTCGGCCCAGCGTAAGAAATCTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGC  >  NC_000913/476892‑477158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑