Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,416,763	2 bp→TC	coding (2017‑2018/2145 nt)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,416,763	0	C	T	100.0%	151.0 / NA	47	L673S (CTG→TCG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/22);  total (25/22)
*	NC_000913	2,416,764	0	T	C	100.0%	168.4 / NA	47	L673S (CTG→TCG)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/22);  total (25/22)

CTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCT  >  NC_000913/2416632‑2416894                                                                                                                                    ||                                                                                                                                   cTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGAtctc                                                                                                                              >  2:1089117/1‑139 (MQ=255)             cTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAt                                                                                                                  <  1:1089117/139‑1 (MQ=255)                                            tcCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGAc                                                                                   <  2:552379/139‑1 (MQ=255)                                                          gcgcTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGgcgc                                                                     <  1:520349/139‑1 (MQ=255)                                                                   tgtTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGa                                                            <  2:640292/139‑1 (MQ=255)                                                                                  aTCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTa                                                                                        >  2:1138162/1‑96 (MQ=255)                                                                                  aTCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTa                                                                                        <  1:1138162/96‑1 (MQ=255)                                                                                       aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATcg                                        >  1:915337/1‑139 (MQ=255)                                                                                       aaCACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATcg                                        >  1:584312/1‑139 (MQ=255)                                                                                            cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGTTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTg                                   >  2:757151/1‑139 (MQ=255)                                                                                            cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                       >  2:451959/1‑119 (MQ=255)                                                                                            cGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGata                                                       <  1:451959/119‑1 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCAt                                                                                                                           >  2:305776/1‑49 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCAt                                                                                                                           <  2:529505/49‑1 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCAt                                                                                                                           >  1:529505/1‑49 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCAt                                                                                                                           <  1:305776/49‑1 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAt                                                                                                                  <  2:1133906/58‑1 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGAt                                                                                                                  >  1:1133906/1‑58 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCAc                                                                   >  1:61363/1‑105 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCAc                                                                   <  2:61363/105‑1 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTg                                                             >  1:925198/1‑111 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTg                                                             <  2:925198/111‑1 (MQ=255)                                                                                              gTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGAc                                 >  1:407981/1‑139 (MQ=255)                                                                                                 acacCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATc                                                     >  2:212651/1‑116 (MQ=255)                                                                                                 acacCACCTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATc                                                     <  1:212651/116‑1 (MQ=255)                                                                                                     caccTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGAtt                          <  2:915337/139‑1 (MQ=255)                                                                                                      accTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGa                                                                                                                   >  1:175234/1‑49 (MQ=255)                                                                                                      accTACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGa                                                                                                                   <  2:175234/49‑1 (MQ=255)                                                                                                         tACAAAGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGt                      <  2:407981/139‑1 (MQ=255)                                                                                                             aaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                             <  1:703449/64‑1 (MQ=255)                                                                                                             aaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGcc                                                                                             >  2:703449/1‑64 (MQ=255)                                                                                                             aaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGAc                                                                                   >  2:947686/1‑74 (MQ=255)                                                                                                             aaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGAc                                                                                   <  1:947686/74‑1 (MQ=255)                                                                                                             aaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGat                                                        >  2:295585/1‑101 (MQ=255)                                                                                                             aaGCGGTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGat                                                        <  1:295585/101‑1 (MQ=255)                                                                                                                  gTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGt                                                                                                        >  1:805861/1‑48 (MQ=255)                                                                                                                  gTACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGt                                                                                                        <  2:805861/48‑1 (MQ=255)                                                                                                                   tACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGt                                                                                                        >  1:685849/1‑47 (MQ=255)                                                                                                                   tACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGt                                                                                                        <  2:685849/47‑1 (MQ=255)                                                                                                                   tACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCa                                                                    >  2:393506/1‑83 (MQ=255)                                                                                                                   tACAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCa                                                                    <  1:393506/83‑1 (MQ=255)                                                                                                                     cAGCGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCAcagcagc          >  1:639170/1‑139 (MQ=255)                                                                                                                        cGTTCTGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGt       >  1:1110102/1‑139 (MQ=255)                                                                                                                          ttCTGCCGACTCGATCTCCATCGGGCCGATGCTGCa                                                                                                            <  2:1165952/36‑1 (MQ=255)                                                                                                                          ttCTGCCGACTCGATCTCCATCGGGCCGATGCTGCa                                                                                                            >  1:1165952/1‑36 (MQ=255)                                                                                                                             tGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAAtct  >  2:765204/1‑139 (MQ=255)                                                                                                                             tGCCGACTCGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAAtct  >  1:25397/1‑139 (MQ=255)                                                                                                                                    ||                                                                                                                                   CTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACACCGGTAACACCACCTACAAAGCGGTACAGCGTTCTGCCGACCTGATCTCCATCGGGCCGATGCTGCAGGGTATGCGCAAGCCGGTTAACGACCTGTCCCGTGGCGCACTGGTTGACGATATCGTCTACACCATCGCGCTGACTGCGATTCAGTCTGCACAGCAGCAGTAATCT  >  NC_000913/2416632‑2416894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑