Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,717,821 (C)5→4 coding (708/1038 nt) yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,717,8170C.100.0% 209.0 / NA 52coding (712/1038 nt)yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (27/25);  total (27/25)

CAGCGGTTTACCCTGCTCGCTGGAAGTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCC  >  NC_000913/2717680‑2717949
                                                                                                                                         |                                                                                                                                    
cAGCGGTTTACCCTGCTCGCTGGAAGTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAgccgcc                                                                                                                                     <  1:1057898/139‑1 (MQ=255)
        tACCCTGCTCGCTGGAAGTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAgccgcc                                                                                                                                     >  2:1849554/1‑131 (MQ=255)
        tACCCTGCTCGCTGGAAGTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAgccgcc                                                                                                                                     <  1:1849554/131‑1 (MQ=255)
               cTCGCTGGAAGTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTg                                                                                                                     >  2:805892/1‑139 (MQ=255)
                 cGCTGGAAGTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGcc                                                                                                                   <  1:805892/139‑1 (MQ=255)
                         gTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTg                                                                                                           >  2:1491291/1‑139 (MQ=255)
                            gTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGcac                                                                                                        >  1:298771/1‑139 (MQ=255)
                              cACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGcacca                                                                                                      <  2:1787198/139‑1 (MQ=255)
                                       gtAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                                                                             <  1:1491291/139‑1 (MQ=255)
                                       gtAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                                                                             <  1:29907/139‑1 (MQ=255)
                                            ctgcctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACg                                                                                        <  1:50639/139‑1 (MQ=255)
                                             tgcctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGc                                                                                       >  1:371023/1‑139 (MQ=255)
                                                ctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCa                                                                                                                <  1:1011364/111‑1 (MQ=255)
                                                ctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCa                                                                                                                >  2:1011364/1‑111 (MQ=255)
                                                ctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCa                                                                                                                <  2:1796934/111‑1 (MQ=255)
                                                ctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCa                                                                                                                >  1:1796934/1‑111 (MQ=255)
                                                ctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGcacc                                                                                                       <  2:1113161/120‑1 (MQ=255)
                                                ctgACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGcacc                                                                                                       >  1:1113161/1‑120 (MQ=255)
                                                        aaTCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTgcgcg                                                                            <  2:674907/139‑1 (MQ=255)
                                                          tcatcCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCa                                                                          <  1:1815214/139‑1 (MQ=255)
                                                              ccAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTg                                                                      >  2:1808634/1‑139 (MQ=255)
                                                                  cACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGca                                                                  >  1:1807431/1‑139 (MQ=255)
                                                                      ttAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGcatcat                                                              <  2:269458/139‑1 (MQ=255)
                                                                        aaCAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGc                                                            <  2:371023/139‑1 (MQ=255)
                                                                            aTGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTc                                                                                           <  2:122639/104‑1 (MQ=255)
                                                                            aTGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTc                                                                                           >  1:122639/1‑104 (MQ=255)
                                                                              gttgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCa                                                      >  2:503112/1‑139 (MQ=255)
                                                                               ttgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAc                                                         <  1:1230194/135‑1 (MQ=255)
                                                                               ttgtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTCCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAc                                                         >  2:1230194/1‑135 (MQ=255)
                                                                                 gtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGccccgac                                                                                                                                <  2:1650461/63‑4 (MQ=255)
                                                                                 gtCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGccccgac                                                                                                                                >  1:1650461/1‑60 (MQ=255)
                                                                                    gCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGcc                                                                                                                   >  1:1589645/1‑72 (MQ=255)
                                                                                    gCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGcc                                                                                                                   <  2:1589645/72‑1 (MQ=255)
                                                                                              ggCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGa                                      >  1:854662/1‑139 (MQ=255)
                                                                                                cTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAccc                                                       >  1:1853129/1‑120 (MQ=255)
                                                                                                cTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAccc                                                       <  2:1853129/120‑1 (MQ=255)
                                                                                                 tGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                                                                             <  2:1823823/81‑1 (MQ=255)
                                                                                                 tGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                                                                             >  1:1823823/1‑81 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGcc                                                                                                                   >  1:308334/1‑57 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGcc                                                                                                                   <  2:308334/57‑1 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCGTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGca                                                                  >  1:1224644/1‑106 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGcacca                                                                                                      >  1:1821067/1‑70 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGcacca                                                                                                      <  2:1821067/70‑1 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACGCCTTTCACGCCGAAGTGCGCGCAGCTGCGca                                                                  <  2:1224644/106‑1 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTg                                                                      <  1:1177646/102‑1 (MQ=255)
                                                                                                   aaCGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTg                                                                      >  2:1177646/1‑102 (MQ=255)
                                                                                                         ctctGCGCCGCCTTCTGCGGTACGGATAGCCGcccc                                                                                                                                   <  1:527672/36‑1 (MQ=255)
                                                                                                         ctctGCGCCGCCTTCTGCGGTACGGATAGCCGcccc                                                                                                                                   >  2:527672/1‑36 (MQ=255)
                                                                                                          tctGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATTCGTTTTCCAg                          >  2:1674076/1‑139 (MQ=255)
                                                                                                          tctGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTAATGCGGGTTAGATTCGTTTTCCAg                          >  2:1048319/1‑139 (MQ=255)
                                                                                                           ctGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                                                                             <  1:1776200/71‑1 (MQ=255)
                                                                                                           ctGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCtt                                                                                             >  2:1776200/1‑71 (MQ=255)
                                                                                                               gccgccTTCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCa                     <  2:1807431/139‑1 (MQ=255)
                                                                                                                      tCTGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCa              >  2:233167/1‑139 (MQ=255)
                                                                                                                        tGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAc                                                         >  2:674985/1‑94 (MQ=255)
                                                                                                                        tGCGGTACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCAc                                                         <  1:674985/94‑1 (MQ=255)
                                                                                                                             tACGGATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATCGTTTTCCAGTGCCAGAACGCAATCCTgcg      >  1:1374363/1‑139 (MQ=255)
                                                                                                                                 gATAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTgcgcgc   <  1:1808634/139‑1 (MQ=255)
                                                                                                                                  aTAGCCGCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCgcc  >  1:297033/1‑139 (MQ=255)
                                                                                                                                         |                                                                                                                                    
CAGCGGTTTACCCTGCTCGCTGGAAGTGGTCACTACGGTGTAACCTGCCTGACGGAAATCATCCAGCACGTTAACAATGTTGTCGCCAGTAATCGGCTGAACGTGCTCTGCGCCGCCTTCTGCGGTACGGATAGCCGCCCCCGACTCCAGCAGTGCCGCATCCTGCACCACGACACCTTTCACGCCGAAGTGCGCGCAGCTGCGCATCATGCCACCCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCC  >  NC_000913/2717680‑2717949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: