Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 281,662 Δ1 bp coding (322/1155 nt) yagA ← CP4‑6 prophage, putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913281,6600G.100.0% 133.2 / NA 34coding (324/1155 nt)yagACP4‑6 prophage, putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (15/19);  total (15/19)

GTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGG  >  NC_000913/281528‑281788
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gTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATgg                                                                                                                           >  1:126939/1‑139 (MQ=255)
      cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGcc                                                                                                                     >  1:639881/1‑139 (MQ=255)
                 tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggca                                                                                                                               >  2:1447205/1‑117 (MQ=255)
                 tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCggca                                                                                                                               <  1:1447205/119‑3 (MQ=255)
                 tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCg                                                                                                          >  2:393855/1‑139 (MQ=255)
                             ggTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTa                                                                                              <  1:1354943/139‑1 (MQ=255)
                                  ggCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCtt                                                                                         <  1:393855/139‑1 (MQ=255)
                                      cgtcgtGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCgg                                                                                     <  1:1040117/139‑1 (MQ=255)
                                            gtTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTccc                                                                               <  2:126939/139‑1 (MQ=255)
                                            gtTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTccc                                                                               <  1:784197/139‑1 (MQ=255)
                                             ttCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTcccc                                                                              <  1:536767/139‑1 (MQ=255)
                                                      gCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCAt                                                                     >  2:234660/1‑139 (MQ=255)
                                                        ccGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGa                                                                   <  1:1502624/139‑1 (MQ=255)
                                                              cGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCa                                                             <  2:1042710/139‑1 (MQ=255)
                                                                ggAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGc                                                                                                       <  2:1217028/95‑1 (MQ=255)
                                                                ggAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATg                                                           <  2:1262225/139‑1 (MQ=255)
                                                                ggAATGCCCGGTGAAGCGCCCGGCAGCAAGCCATCGCGGGCCATCAGGTCATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGc                                                                                                       >  1:1217028/1‑95 (MQ=255)
                                                                 gAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGcccc                                                                                                                   <  2:1830498/82‑1 (MQ=255)
                                                                 gAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGcccc                                                                                                                   >  1:1830498/1‑82 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTg                                                                                                                 <  2:1569167/46‑1 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTg                                                                                                                 >  1:1569167/1‑46 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGc                                                                                   >  1:697060/1‑76 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGc                                                                                   <  2:697060/76‑1 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGcc                                        <  1:669027/119‑1 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGcc                                        >  2:669027/1‑119 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGc                     <  1:634600/138‑1 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGc                     >  2:634600/1‑138 (MQ=255)
                                                                                                       gCCATCAGGTTATGGACGGTGCTGAAGGCGGCAGGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCg                    <  1:1548508/139‑1 (MQ=255)
                                                                                                            cAGGTTATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCg               <  2:639881/139‑1 (MQ=255)
                                                                                                                ttATGGACGGTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGa           <  1:234660/139‑1 (MQ=255)
                                                                                                                   tGGACGGTGCTGAAGGCGCCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATg        >  1:1044406/1‑139 (MQ=255)
                                                                                                                         gTGCTGAATGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCgg  >  1:656656/1‑139 (MQ=255)
                                                                                                                         gTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCgg  >  1:948050/1‑139 (MQ=255)
                                                                                                                         gTGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCgg  >  1:1588307/1‑139 (MQ=255)
                                                                                                                          tGCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCAt                                                            >  2:1421223/1‑80 (MQ=255)
                                                                                                                          ggCTGAAGGCGGCATGGTGTGCCCCTGGTCCTCGGGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCAt                                                            <  1:1421223/79‑1 (MQ=255)
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GTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCCTGGTCCTCGAGCCAGCGCTTAATCTTGCGGGCTCCCCAGCGTTCATGACGGTCATGGGCCATACGCAGCAGGGCCGTGATGTCGTCAGATGAGCGGTTCGGGGAATGGTGCGG  >  NC_000913/281528‑281788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: