Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,815,883 Δ1 bp pseudogene (667/669 nt) rph ← ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8800C.98.4% 241.2 / ‑2.5 61pseudogene (2/48 nt)rphribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base . (31/29);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

AAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTT  >  NC_000913/3815745‑3816014
                                                                                                                                        |                                                                                                                                      
aaaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccct                                                                                                                                     <  2:104771/139‑2 (MQ=255)
     ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGc                                                                                                                               <  2:677968/139‑1 (MQ=255)
       cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCa                                                                                                                             >  1:331048/1‑139 (MQ=255)
             tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCca                                                                                                                       <  2:331048/139‑1 (MQ=255)
                     cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccct                                                                                                                                     <  1:1111923/118‑2 (MQ=255)
                     cATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccct                                                                                                                                     >  2:1111923/1‑117 (MQ=255)
                      aTGCCTTCGCTCCTCATCTTCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                             >  1:1581668/1‑139 (MQ=255)
                         ccTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTa                                                                                                           >  1:1186247/1‑139 (MQ=255)
                         ccTTCGCTCCTCATCTTCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  2:1581668/139‑1 (MQ=255)
                          cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAACTCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  1:1350910/139‑1 (MQ=255)
                          cTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  2:1186247/139‑1 (MQ=255)
                           ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAg                                                                                                                  <  2:501003/130‑1 (MQ=255)
                           ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAg                                                                                                                  >  1:501003/1‑130 (MQ=255)
                            tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  1:414168/137‑1 (MQ=255)
                            tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          >  2:414168/1‑137 (MQ=255)
                                          aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGcc                                                                                                                        >  2:1440537/1‑109 (MQ=255)
                                          aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGcc                                                                                                                        <  1:1440537/109‑1 (MQ=255)
                                          aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAg                                                                                                                  >  2:1435385/1‑115 (MQ=255)
                                          aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAg                                                                                                                  <  1:1435385/115‑1 (MQ=255)
                                          aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                     >  2:820988/1‑128 (MQ=255)
                                          aCTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                     <  1:820988/128‑1 (MQ=255)
                                                 tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                   >  2:1513322/1‑139 (MQ=255)
                                                      aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccct                                                                                                                                     <  2:136455/85‑2 (MQ=255)
                                                      aCAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccct                                                                                                                                     >  1:136455/1‑84 (MQ=255)
                                                          aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGc                                                                                                                               <  2:296892/86‑1 (MQ=255)
                                                          aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGc                                                                                                                               >  1:296892/1‑86 (MQ=255)
                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGc                                                                  >  1:1056800/1‑139 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCag                                                                                                                            >  2:1804372/1‑79 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCag                                                                                                                            <  1:1804372/79‑1 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                             <  2:1797435/94‑1 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                             >  1:1797435/1‑94 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  2:852398/97‑1 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          >  1:852398/1‑97 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                       <  2:596255/116‑1 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGc                                                                                       >  1:596255/1‑116 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                                                                        >  1:502129/1‑131 (MQ=255)
                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                                                                        <  2:502129/131‑1 (MQ=255)
                                                                     cTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCGAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACt                                                               >  1:1207377/1‑139 (MQ=255)
                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  1:1056145/125‑1 (MQ=255)
                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   >  2:1056145/1‑125 (MQ=255)
                                                                                ccTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcg                                                    >  1:1649179/1‑139 (MQ=255)
                                                                                  ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  2:1675925/83‑1 (MQ=255)
                                                                                  ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          >  1:1675925/1‑83 (MQ=255)
                                                                                  ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc                                                  <  2:100118/139‑1 (MQ=255)
                                                                                  ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGcc                                                  >  2:282862/1‑139 (MQ=255)
                                                                                       aaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  2:1647375/78‑1 (MQ=255)
                                                                                       aaTCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          >  1:1647375/1‑78 (MQ=255)
                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCagag                                                                                                                          >  1:257112/1‑58 (MQ=255)
                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCagag                                                                                                                          <  2:257112/58‑1 (MQ=255)
                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCag                                                                                                                            >  1:263848/1‑56 (MQ=255)
                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCag                                                                                                                            <  2:263848/56‑1 (MQ=255)
                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          <  1:55306/74‑1 (MQ=255)
                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                          >  2:55306/1‑74 (MQ=255)
                                                                                              ttGCCAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtca                                      <  2:1056800/139‑1 (MQ=255)
                                                                                                 ccAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                   >  1:588458/1‑139 (MQ=255)
                                                                                                  cAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccct                                                                                                                                     >  2:849966/1‑40 (MQ=39)
                                                                                                  cAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTccct                                                                                                                                     <  1:849966/41‑2 (MQ=38)
                                                                                                  cAGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCAc                                  >  1:1363773/1‑139 (MQ=255)
                                                                                                     cgccgcCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                  <  2:1817977/88‑1 (MQ=255)
                                                                                                     cgccgcCTTCTGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                  >  1:1817977/1‑88 (MQ=255)
                                                                                                          ccTTCTGCGTCGCTACAATGGATTCGATTCCCCCTCGGGCCAGCGCCAACAAGGTGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGtt                            >  2:1829372/1‑139 (MQ=255)
                                                                                                               tGCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                     <  1:1513322/139‑1 (MQ=255)
                                                                                                                gCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:211033/92‑1 (MQ=255)
                                                                                                                gCGTCGTTACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   >  1:211033/1‑92 (MQ=255)
                                                                                                                    cgttACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGtctc                >  1:557461/4‑139 (MQ=255)
                                                                                                                      ttACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   <  2:568346/85‑1 (MQ=255)
                                                                                                                      ttACAATGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                   >  1:568346/2‑86 (MQ=255)
                                                                                                                           aTGGATTCGATTCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCa         <  1:282862/139‑1 (MQ=255)
                                                                                                                              gattcgattcCCTCGGGCCAGAGCCGACAAGATGAg                                                                                                             <  2:497930/36‑1 (MQ=255)
                                                                                                                              gattcgattcCCTCGGGCCAGAGCCGACAAGATGAg                                                                                                             >  1:497930/1‑36 (MQ=255)
                                                                                                                              gattcgattcCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                           <  2:869067/118‑1 (MQ=255)
                                                                                                                              gattcgattcCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTc                           >  1:869067/1‑118 (MQ=255)
                                                                                                                                 tcgattcCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                     >  2:1813342/1‑73 (MQ=255)
                                                                                                                                 tcgattcCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                     <  1:1813342/73‑1 (MQ=255)
                                                                                                                                 tcgattcCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt   >  1:95456/1‑139 (MQ=255)
                                                                                                                                 tcgattcCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCt   >  1:1295235/1‑139 (MQ=255)
                                                                                                                                  cgattcCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCtt  <  2:1207377/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTT  >  NC_000913/3815745‑3816014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: