Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,432,994 (C)6→7 coding (567/1125 nt) smf ← DNA recombination‑mediator A family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,432,9881.C98.4% 215.1 / ‑4.0 63coding (573/1125 nt)smfDNA recombination‑mediator A family protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (31/31);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTAC  >  NC_000913/3432865‑3433110
                                                                                                                             |                                                                                                                              
aCCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAg                                                                                                                  >  2:269275/1‑139 (MQ=255)
  cACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg                                                                                                                                 <  1:1873410/122‑1 (MQ=255)
  cACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAgcg                                                                                                                                 >  2:1873410/1‑122 (MQ=255)
            aGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGc                                                                                                      >  2:539344/1‑139 (MQ=255)
              ccGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑Ca                                                                                                >  2:1742752/1‑139 (MQ=255)
                           caGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGAcg                                                                                   >  2:245717/1‑139 (MQ=255)
                            aGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGAcgg                                                                                  <  1:930249/139‑1 (MQ=255)
                              tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTcagtcgggcatgac                                                                                >  1:77129/1‑126 (MQ=255)
                              tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCAt                                                                                       >  2:275456/1‑132 (MQ=255)
                              tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCAt                                                                                       <  1:275456/132‑1 (MQ=255)
                              tACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGAcggcg                                                                                <  1:269275/139‑1 (MQ=255)
                                 acCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAAGATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCgggg                                                                             <  1:1347447/139‑1 (MQ=255)
                                 acCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAAATGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCgggg                                                                             <  1:1842323/139‑1 (MQ=255)
                                   cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGc                                                                                                      >  1:824774/1‑116 (MQ=255)
                                   cTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGc                                                                                                      <  2:824774/116‑1 (MQ=255)
                                     ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGa                                                                         >  2:1444875/1‑139 (MQ=255)
                                     ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGa                                                                         <  2:1214733/139‑1 (MQ=255)
                                     ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGccccc                                                                                                                           <  1:1667121/92‑1 (MQ=255)
                                     ttACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGccccc                                                                                                                           >  2:1667121/1‑92 (MQ=255)
                                      tACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGaa                                                                        <  2:293310/139‑1 (MQ=255)
                                        cTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATg                                                                      >  1:786381/1‑139 (MQ=255)
                                          tAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGCCAGTCAGTCGGGCATGACGGCGGGGATGaa                                                                        <  2:77129/139‑1 (MQ=255)
                                             aCCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTAtt                                                                 >  2:856464/1‑139 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCATC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                            >  2:1405595/1‑139 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTc                                                                                             >  1:515296/1‑106 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTc                                                                                             <  2:515296/106‑1 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGAcggcg                                                                                <  1:1052285/119‑1 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGAcggcg                                                                                >  2:1052285/1‑119 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGAt                                                                           >  2:1757907/1‑124 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGAt                                                                           <  1:1757907/124‑1 (MQ=255)
                                                  tGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                            <  1:1405595/139‑1 (MQ=255)
                                                    aTAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                          <  2:786381/139‑1 (MQ=255)
                                                       atacGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCATGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                       <  1:1244862/136‑1 (MQ=255)
                                                       aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGaa                                                                        >  1:1884463/1‑122 (MQ=255)
                                                       aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGaa                                                                        <  2:1884463/122‑1 (MQ=255)
                                                       aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                            >  2:1397763/1‑134 (MQ=255)
                                                       aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTc                                                            <  1:1397763/134‑1 (MQ=255)
                                                       aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTc                                                       >  2:504168/1‑139 (MQ=255)
                                                       aTGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACAGCGAGGGGAAATTCAAAGACGAGAGCGGCGCCCCGGAGCAAGCAGGGTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGGATGGAATAAAGTTCAtgc                                                       >  1:820927/1‑137 (MQ=255)
                                                            aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCaa                                                  <  1:616301/139‑1 (MQ=255)
                                                            aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGccccc                                                                                                                           >  1:184950/1‑69 (MQ=255)
                                                            aTTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGccccc                                                                                                                           <  2:184950/69‑1 (MQ=255)
                                                              ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGa                                                                         >  2:1645479/1‑114 (MQ=255)
                                                              ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGa                                                                         <  1:1645479/114‑1 (MQ=255)
                                                              ttcttcGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATa                                                <  1:822251/139‑1 (MQ=255)
                                                                   cGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAAt                                                                       <  1:578893/111‑1 (MQ=255)
                                                                   cGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAAt                                                                       >  2:578893/1‑111 (MQ=255)
                                                                       ggAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGc                                       >  2:1072521/1‑139 (MQ=255)
                                                                          aaTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGa                                    <  1:504168/139‑1 (MQ=255)
                                                                           aTTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGAc                                   >  2:700832/1‑139 (MQ=255)
                                                                                      ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATa                                                <  1:206376/115‑1 (MQ=255)
                                                                                      ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCt                        >  1:909879/1‑139 (MQ=255)
                                                                                      ggggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATAAAGTCCATTCCCCAATa                                                >  2:206376/1‑115 (MQ=255)
                                                                                       gggTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                               <  2:1632682/38‑1 (MQ=255)
                                                                                       gggTGGAACATCGAGGGGAAATTCCGAGACGAGAgcgc                                                                                                                               >  1:1632682/1‑38 (MQ=255)
                                                                                       gggTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTg                       <  2:644560/139‑1 (MQ=255)
                                                                                                  cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                          <  2:824302/93‑1 (MQ=255)
                                                                                                  cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCa                                                          >  1:824302/1‑93 (MQ=255)
                                                                                                  cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                     >  1:532989/1‑98 (MQ=255)
                                                                                                  cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTccc                                                     <  2:532989/98‑1 (MQ=255)
                                                                                                  cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCtt            >  2:86756/1‑139 (MQ=255)
                                                                                                  cGAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCtt            >  2:747717/1‑139 (MQ=255)
                                                                                                   gAGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCttt           >  2:511966/1‑139 (MQ=255)
                                                                                                    aGGGGAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTa          <  1:1742752/139‑1 (MQ=255)
                                                                                                      gggAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCgggg                                                                             >  2:881701/1‑70 (MQ=255)
                                                                                                      gggAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCgggg                                                                             <  1:881701/70‑1 (MQ=255)
                                                                                                      gggAAATTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATg        <  1:856464/139‑1 (MQ=255)
                                                                                                          aaTTCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCt    <  1:511966/139‑1 (MQ=255)
                                                                                                            ttCCGAGACGAGAGCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTac  >  2:1383448/1‑139 (MQ=255)
                                                                                                                      agagCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCgggg                                                                             <  1:163579/54‑1 (MQ=255)
                                                                                                                      agagCGCCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCgggg                                                                             >  2:163579/1‑54 (MQ=255)
                                                                                                                             |                                                                                                                              
ACCACTACGCAAAGCCGCTTCCACCACCAGTACACCTTTACTTAGACCACTGATAATGCGATTTCTTCGTGGGAAATTGTAAGCAAGGGGTGGAACATCGAGGGGAAATTCCGAGACGAGAGCGCCCCCCTGTTCAAGCAGACTGGCAGC‑‑‑‑CAGTCGGGCATGACGGCGGGGATGAATGGTATTAAGTCCATTCCCCAATACAGCAATGCTGACGCCATTTACCTGTAAGGCTGCTTTATGCGCTAC  >  NC_000913/3432865‑3433110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: