Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,511,746 (A)8→9 coding (599/906 nt) yhfZ ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,511,7381.A98.0% 317.6 / ‑1.6 98coding (607/906 nt)yhfZputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base A (53/43);  total (55/43)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

Only 100 of 109 total aligned reads displayed.

GCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511603‑3511871
                                                                                                                                        |                                                                                                                                        
gcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaa                                                                                                                                      >  1:1589451/1‑139 (MQ=255)
 cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaa                                                                                                                                     >  1:1715967/1‑139 (MQ=255)
        tctAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaaca                                                                                                                             <  1:742296/139‑4 (MQ=255)
             tCCCACCATGCTCAGTTCGTTTTCCTCCACCACGTTCCAGATCACCGCATCGACATCCCCTTTACCAATGCGTTTTAAACTCTCGTGATAAGATAGATCTACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGt                                                                                                                        <  1:1177101/139‑1 (MQ=255)
                 aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATg                                                                                                                    >  2:1177101/1‑139 (MQ=255)
                 aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATg                                                                                                                    >  2:1526945/1‑139 (MQ=255)
                 aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATg                                                                                                                    <  2:1437154/139‑1 (MQ=255)
                        tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCt                                                                                                             >  2:53627/1‑139 (MQ=255)
                            ttCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATc                                                                                                         >  2:190159/1‑139 (MQ=255)
                             tCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGCTcc                                                                                                        >  2:1797878/1‑139 (MQ=255)
                                 tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  1:662989/139‑1 (MQ=255)
                                    ccGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAAc                                                                                                 <  1:770427/139‑1 (MQ=255)
                                      gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               <  2:1715967/139‑1 (MQ=255)
                                          ccacGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGCTCCGCCGAACGGTTAt                                                                                           >  2:897994/1‑139 (MQ=255)
                                             cGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCa                                                                                        >  2:817305/1‑139 (MQ=255)
                                                     aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg                                                                                <  1:1526945/139‑1 (MQ=255)
                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTTCACATCACTACCGCC‑‑aaaaaaaaaca                                                                                                                             >  1:1233610/1‑86 (MQ=255)
                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                           >  2:8479/1‑139 (MQ=255)
                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt                                                                           <  1:1797878/139‑1 (MQ=255)
                                                          cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaaca                                                                                                                             <  2:1233610/89‑4 (MQ=255)
                                                            cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTc                                                                          >  2:422100/1‑139 (MQ=255)
                                                            cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaaca                                                                                                                             <  1:1521034/87‑4 (MQ=255)
                                                                   aTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGCTTTTCTGATccgccg                                                                                                    >  1:251244/1‑105 (MQ=255)
                                                                   aTCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    <  2:251244/105‑1 (MQ=255)
                                                                    tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCCCTACCGCCAAAAAAAAACATCAGTCATGATTTTCTGATCCGCCGAACGATTATCCAGCCCCACGCGCTTCACGTTTgtg                                                                 >  2:527893/1‑137 (MQ=255)
                                                                    tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg                                                                 >  2:1675652/1‑139 (MQ=255)
                                                                     cGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACCATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCgg                                                                >  2:836319/1‑139 (MQ=255)
                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc                                                          >  2:779219/1‑139 (MQ=255)
                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc                                                          >  1:122201/1‑139 (MQ=255)
                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaacat                                                                                                                            >  2:1030923/1‑69 (MQ=255)
                                                                           tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaacat                                                                                                                            <  1:1030923/73‑5 (MQ=255)
                                                                            aaCAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGAtt                                                                                                                 >  2:1864109/1‑83 (MQ=255)
                                                                            aaCAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGAtt                                                                                                                 <  1:1864109/83‑1 (MQ=255)
                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                    >  2:1450833/1‑123 (MQ=255)
                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt                                                                    <  1:1450833/123‑1 (MQ=255)
                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    >  2:1085696/1‑139 (MQ=255)
                                                                                 tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    >  2:123787/1‑139 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGCCTCGTTCCACATCACTACCGCC‑‑AAAAAAACACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                                  >  2:1842583/1‑92 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGaa                                                                                                  <  1:1842583/92‑1 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                  <  1:1002585/108‑1 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                   >  1:558029/1‑139 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                   >  2:1428731/1‑139 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACg                                                   <  1:123787/139‑1 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGAACCGCCGAACGGTTATCCAGCCCCa                                                                                  >  2:1002585/1‑108 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaa                                                                                                                               >  2:1562236/1‑62 (MQ=255)
                                                                                  gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aaaaaaaaa                                                                                                                               <  1:1562236/63‑2 (MQ=255)
                                                                                    gTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 <  2:1589451/139‑1 (MQ=255)
                                                                                           aCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCaa                                          >  2:525726/1‑139 (MQ=255)
                                                                                            ctctCGTGATAAGAGAGATCGACTCGTCCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc                                         <  1:1085696/139‑1 (MQ=255)
                                                                                             tctcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATccgccg                                                                                                    >  2:683184/1‑79 (MQ=255)
                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               <  2:1264570/82‑1 (MQ=255)
                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACgg                                                                                               >  1:1264570/1‑82 (MQ=255)
                                                                                               tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTgg                                      >  1:1790919/1‑139 (MQ=255)
                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            >  2:936509/1‑112 (MQ=255)
                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            <  1:936509/112‑1 (MQ=255)
                                                                                                    aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc                                 <  2:1018954/139‑1 (MQ=255)
                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:1675652/139‑1 (MQ=255)
                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:190159/139‑1 (MQ=255)
                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:1420904/139‑1 (MQ=255)
                                                                                                      aaGAGAGATCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:566438/139‑1 (MQ=255)
                                                                                                       agagagATCGACTCGTTCCACATCACTACCGCCAAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:422100/139‑1 (MQ=255)
                                                                                                         agagaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAAc                                         >  2:1635759/1‑126 (MQ=255)
                                                                                                           agaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCgg                                                                                                                         >  2:306029/1‑44 (MQ=255)
                                                                                                           agaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCgg                                                                                                                         <  1:306029/44‑1 (MQ=38)
                                                                                                            gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                    <  1:1321922/128‑1 (MQ=255)
                                                                                                            gaTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg                                    >  2:1321922/1‑128 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      <  1:1792679/45‑1 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      >  2:1792679/1‑45 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            <  1:914598/103‑1 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            >  2:914598/1‑103 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                        >  1:1182689/1‑139 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa                        >  1:472213/1‑139 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCAGTCATGTTTTTCTGATCCGCCGAAc                                                                                                 >  2:1289571/1‑66 (MQ=255)
                                                                                                             aTCGACTCGTTCCACATCACTACCACCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGc                               <  1:1226258/132‑1 (MQ=255)
                                                                                                              tCGACTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAg                       <  1:525726/139‑1 (MQ=255)
                                                                                                                 aCTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      <  1:585517/41‑1 (MQ=255)
                                                                                                                 aCTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCa                                                                                                                      >  2:585517/1‑41 (MQ=255)
                                                                                                                  cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             <  1:885555/97‑1 (MQ=255)
                                                                                                                  cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             >  2:885555/1‑97 (MQ=255)
                                                                                                                  cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            <  2:164212/98‑1 (MQ=255)
                                                                                                                  cTCGTTCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTc                                                            >  1:164212/1‑98 (MQ=255)
                                                                                                                     ggtCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg                >  1:1127464/3‑139 (MQ=255)
                                                                                                                       tCCACATCACTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGc              <  1:1428731/139‑1 (MQ=255)
                                                                                                                             tCACTACCGCCAAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCaa         <  1:1056981/139‑1 (MQ=255)
                                                                                                                                cTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    <  2:57886/92‑1 (MQ=255)
                                                                                                                                cTACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc                                                    >  1:57886/1‑92 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGAt                                                                                                          <  1:1496089/37‑1 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGAt                                                                                                          >  2:1496089/1‑37 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             <  2:518949/82‑1 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt                                                             >  1:518949/1‑82 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 <  2:27985/94‑1 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc                                                 >  1:27985/1‑94 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg                <  1:1567556/127‑1 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg                >  2:1567556/1‑127 (MQ=255)
                                                                                                                                 tACCGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa    >  1:504127/1‑139 (MQ=255)
                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                  >  1:1383672/1‑59 (MQ=255)
                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCa                                                                                  <  2:1383672/59‑1 (MQ=255)
                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc  >  2:402261/1‑139 (MQ=255)
                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc  >  2:1457610/1‑139 (MQ=255)
                                                                                                                                   ccGCCAAAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc  >  1:933849/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AAAAAAAACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC  >  NC_000913/3511603‑3511871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: