Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,664,363 (G)6→7 coding (256/729 nt) gadW ← transcriptional activator of gadA and gadBC, repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,664,3571.G97.7% 291.0 / ‑1.3 85coding (262/729 nt)gadWtranscriptional activator of gadA and gadBC, repressor of gadX
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (41/42);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 104 total aligned reads displayed.

ATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAT  >  NC_000913/3664223‑3664479
                                                                                                                                       |                                                                                                                          
aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggg                                                                                                                        >  1:1489279/1‑139 (MQ=255)
        gTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                 <  2:991724/139‑1 (MQ=255)
           aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggg                                                                                                                      <  2:478819/130‑1 (MQ=255)
           aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggg                                                                                                                      >  1:478819/1‑130 (MQ=255)
               gATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGttaat                                                                                                          >  2:45870/1‑139 (MQ=255)
                aTCAGTTCTTTCTTATGAGAAAACATCGATAAACTGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                         >  2:659991/1‑139 (MQ=255)
                aTCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                         <  2:729976/139‑1 (MQ=255)
                aTCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                         <  2:729975/139‑1 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAAc                                                                                                      >  2:654443/1‑139 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAAc                                                                                                      >  2:398696/1‑139 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAAc                                                                                                      >  1:249714/1‑139 (MQ=255)
                   aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATAGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAAc                                                                                                      >  1:1884660/1‑139 (MQ=255)
                     ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑ggggg                                                                                                                       <  2:1067691/119‑1 (MQ=255)
                     ttctttctTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑ggggg                                                                                                                       >  1:1067691/1‑119 (MQ=255)
                          tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                               <  1:652997/139‑1 (MQ=255)
                          tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                               >  2:652997/1‑139 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAACGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggtga                                                                                                                  >  2:1809912/1‑110 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          >  1:799924/1‑139 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          <  1:767018/139‑1 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          <  2:204448/139‑1 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggtga                                                                                                                  <  1:1809912/114‑5 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggt                                                                                                                    <  2:1646550/112‑3 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggt                                                                                                                    >  1:1646550/1‑110 (MQ=255)
                               tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCATCACCGGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          >  2:1361172/1‑139 (MQ=255)
                                gagaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCATCACCGGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGc                                                                                         <  1:1361172/139‑1 (MQ=255)
                                 agaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                               >  2:1327762/1‑132 (MQ=255)
                                 agaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGt                                                                                               <  1:1327762/132‑1 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  1:654443/139‑1 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  1:1379981/139‑1 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  2:1489279/139‑1 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  1:395611/139‑1 (MQ=255)
                                    aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  2:536806/139‑1 (MQ=255)
                                            aTAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  1:426211/131‑1 (MQ=255)
                                            aTAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  2:426211/1‑131 (MQ=255)
                                            aTAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggtg                                                                                                                   <  2:1614030/100‑4 (MQ=255)
                                            aTAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggtg                                                                                                                   >  1:1614030/1‑97 (MQ=255)
                                             tAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTaa                                                                            >  2:466689/1‑139 (MQ=255)
                                                    gAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                 >  2:1683396/1‑95 (MQ=255)
                                                    gAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                 <  1:1683396/95‑1 (MQ=255)
                                                         gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                <  1:45870/139‑1 (MQ=255)
                                                           agagCATATTGCTCAGTATAGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCt                                                              <  2:1884660/139‑1 (MQ=255)
                                                            gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGa                                                            >  1:918828/1‑139 (MQ=255)
                                                              gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGAc                                                           >  1:1747266/1‑139 (MQ=255)
                                                                  atTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                       >  2:1517511/1‑139 (MQ=255)
                                                                   ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAAc                                                                                                  <  2:445578/95‑1 (MQ=255)
                                                                   ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAAc                                                                                                  >  1:445578/1‑95 (MQ=255)
                                                                   ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggg                                                                                                                        <  1:874316/72‑1 (MQ=255)
                                                                   ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggg                                                                                                                        >  2:874316/1‑72 (MQ=255)
                                                                    tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                             <  2:1044344/67‑1 (MQ=255)
                                                                    tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAcc                                                                                >  2:1531772/1‑112 (MQ=255)
                                                                    tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAcc                                                                                <  1:1531772/112‑1 (MQ=255)
                                                                    tGCTCAGTATCGGTGAATGTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                             >  1:1044344/1‑67 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaaa                                                                                                   >  1:924336/1‑86 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaaa                                                                                                   <  2:924336/86‑1 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  2:1051182/100‑1 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  1:1051182/1‑100 (MQ=255)
                                                                           tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaa                                              >  2:242072/1‑139 (MQ=255)
                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggg                                                                                                                        <  1:1509345/61‑1 (MQ=255)
                                                                              cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggg                                                                                                                        >  2:1509345/1‑61 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGt                                                                                                              >  2:1438659/1‑70 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGt                                                                                                              <  1:1438659/70‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          >  1:272941/1‑90 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          <  2:272941/90‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGc                                                               >  1:250646/1‑117 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGc                                                               <  2:250646/117‑1 (MQ=255)
                                                                                gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAAt                                         >  1:997550/1‑139 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggt                                                                                                                    <  2:869647/58‑3 (MQ=255)
                                                                                     tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑gggggggt                                                                                                                    >  1:869647/1‑56 (MQ=255)
                                                                                         ccccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTg                                <  2:799924/139‑1 (MQ=255)
                                                                                         ccccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTg                                >  1:1294464/1‑139 (MQ=255)
                                                                                           ccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                 >  2:1421901/1‑56 (MQ=255)
                                                                                           ccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACt                                                                                                                 <  1:1421901/56‑1 (MQ=255)
                                                                                             ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGt                                                                                                              <  1:18308/57‑1 (MQ=255)
                                                                                             ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGt                                                                                                              >  2:18308/1‑57 (MQ=255)
                                                                                             ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                    >  1:1539276/1‑67 (MQ=255)
                                                                                             ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                    <  2:1539276/67‑1 (MQ=255)
                                                                                             gatagcATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAg                            <  1:1124302/134‑1 (MQ=255)
                                                                                               aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                    <  1:1294934/65‑1 (MQ=255)
                                                                                               aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACaa                                                                                                    >  2:1294934/1‑65 (MQ=255)
                                                                                               aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                          <  1:242072/139‑1 (MQ=255)
                                                                                               aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                          <  1:659991/139‑1 (MQ=255)
                                                                                               aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                          <  2:249714/139‑1 (MQ=255)
                                                                                                   aTTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATc                      >  2:574169/1‑139 (MQ=255)
                                                                                                         gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATc                <  2:1747266/139‑1 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGt                                                                                                              <  1:421408/41‑1 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGt                                                                                                              >  2:421408/1‑41 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  2:769096/1‑66 (MQ=255)
                                                                                                             tCAAAAATCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  1:769096/66‑1 (MQ=255)
                                                                                                                aaaaTCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACGCTAAATTGCGTGGTAGCTGACGACTATTTTGTGAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtcgt         <  2:25496/139‑1 (MQ=255)
                                                                                                                    tCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                <  2:490653/80‑1 (MQ=255)
                                                                                                                    tCGCCGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAg                                                                >  1:490653/1‑80 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                         >  1:738945/1‑36 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGttaatt                                                                                                         <  2:738945/36‑1 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                       <  2:591158/86‑1 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                       >  1:591158/1‑86 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAt  >  1:474968/1‑139 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAt  >  2:1325602/1‑139 (MQ=255)
                                                                                                                       ccGTCACCAGCGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAt  >  2:408971/1‑139 (MQ=255)
                                                                                                                                 cGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  2:534448/1‑46 (MQ=255)
                                                                                                                                 cGGCATGGGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  1:534448/46‑1 (MQ=255)
                                                                                                                                       |                                                                                                                          
ATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑GGGGGGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAT  >  NC_000913/3664223‑3664479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑