Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,922,931 (A)7→8 intergenic (‑491/+126) atpI ← / ← rsmG ATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,922,9241.A95.2% 191.3 / 1.8 62intergenic (‑484/+133)atpI/rsmGATP synthase, membrane‑bound accessory factor/16S rRNA m(7)G527 methyltransferase, SAM‑dependent, glucose‑inhibited cell‑division protein
Reads supporting (aligned to +/- strand):  ref base . (2/1);  new base A (30/29);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.34e-01

TGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑AAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAA  >  NC_000913/3922801‑3923056
                                                                                                                            |                                                                                                                                      
tGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGt                                                                                                                        >  1:1160665/1‑139 (MQ=255)
        acacAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCttgt                                                                                                                <  2:1853890/139‑1 (MQ=255)
              caaAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGc                                                                                                          >  2:1698882/1‑139 (MQ=255)
                 aaaCCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCa                                                                                                         >  2:1859229/1‑137 (MQ=255)
                 aaaCCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCa                                                                                                         <  1:1859229/137‑1 (MQ=255)
                      accCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCa                                                                                                         >  2:425147/1‑132 (MQ=255)
                      accCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCa                                                                                                         <  1:425147/132‑1 (MQ=255)
                        ccATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCttgt                                                                                                                <  1:544755/123‑1 (MQ=255)
                        ccATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCttgt                                                                                                                >  2:544755/1‑123 (MQ=255)
                        ccATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGa                                                                                                >  2:1383213/1‑139 (MQ=255)
                            tGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGt                                                                                            <  1:1783876/139‑1 (MQ=255)
                                  ttttAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACg                                                                                                                         >  1:1263274/1‑104 (MQ=255)
                                  ttttAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACg                                                                                                                         <  2:1263274/104‑1 (MQ=255)
                                           tGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAAc                                                                             <  1:304782/139‑1 (MQ=255)
                                             ttttAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCa                                                                           <  1:497539/139‑1 (MQ=255)
                                                      ccAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑AAAAAAAGcc                                                                                                                            <  1:519352/80‑1 (MQ=255)
                                                      ccAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑AAAAAAAGcc                                                                                                                            >  2:519352/1‑80 (MQ=255)
                                                      ccAATGATGGTTATTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTaa                                                                              >  2:1351884/1‑127 (MQ=255)
                                                      ccAATGATAGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTaa                                                                              <  1:1351884/127‑1 (MQ=255)
                                                       cAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGt                                                                                       >  1:361247/1‑117 (MQ=255)
                                                       cAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGt                                                                                       <  2:361247/117‑1 (MQ=255)
                                                              ggTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGaaaa                                                          >  2:1779193/1‑139 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACg                                                                                                                         <  2:606814/74‑1 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACg                                                                                                                         >  1:606814/1‑74 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCa                                                                           <  1:311378/120‑1 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCa                                                                           >  2:311378/1‑120 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAAcc                                                        >  2:837886/1‑139 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑aaaaaaaagcc                                                                                                                            <  2:865340/71‑5 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑aaaaaaaagcc                                                                                                                            >  1:865340/1‑67 (MQ=255)
                                                                ttCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑AAAAAAATGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAAcc                                                        >  1:1096848/1‑139 (MQ=255)
                                                                 tCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCt                                                       >  1:1120454/1‑139 (MQ=255)
                                                                  cTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGt                                                                                                  <  1:877971/96‑1 (MQ=255)
                                                                  cTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGt                                                                                                  >  2:877971/1‑96 (MQ=255)
                                                                   ttAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTgg                                                     <  1:1698882/139‑1 (MQ=255)
                                                                     aGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑aaaaaaaagc                                                                                                                             <  1:1609411/65‑4 (MQ=255)
                                                                     aGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑aaaaaaaagc                                                                                                                             >  2:1609411/1‑62 (MQ=255)
                                                                        gcCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgtt                                                >  2:444067/1‑139 (MQ=255)
                                                                         ccGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACg                                                                                             >  1:36003/1‑93 (MQ=255)
                                                                         ccGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACg                                                                                             <  2:36003/93‑1 (MQ=255)
                                                                          cGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACg                                                                                                                         <  1:1672051/64‑1 (MQ=255)
                                                                          cGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACg                                                                                                                         >  2:1672051/1‑64 (MQ=255)
                                                                           gATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgttgtt                                             <  1:444067/139‑1 (MQ=255)
                                                                            aTTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTa                                            <  2:1120454/139‑1 (MQ=255)
                                                                            aTTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑aaaaaaaagc                                                                                                                             <  1:738100/58‑4 (MQ=255)
                                                                            aTTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑aaaaaaaagc                                                                                                                             >  2:738100/1‑55 (MQ=255)
                                                                             tttttAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTaa                                           <  2:1160665/139‑1 (MQ=255)
                                                                                       aCTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCaa                                                                                                        >  2:1410852/1‑68 (MQ=255)
                                                                                       aCTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCaa                                                                                                        <  1:1410852/68‑1 (MQ=255)
                                                                                       aCTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGt                                                                                       <  2:312472/85‑1 (MQ=255)
                                                                                       aCTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGt                                                                                       >  1:312472/1‑85 (MQ=255)
                                                                                         tGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTaa                                                                              <  2:934467/92‑1 (MQ=255)
                                                                                         tGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTaa                                                                              >  1:934467/1‑92 (MQ=255)
                                                                                           atatTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTc                             <  1:1779193/139‑1 (MQ=255)
                                                                                                 tCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCa                                                                                                         >  1:82474/1‑57 (MQ=255)
                                                                                                 tCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCa                                                                                                         <  2:82474/57‑1 (MQ=255)
                                                                                                 tCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTgg                                                     >  2:1417030/1‑109 (MQ=255)
                                                                                                 tCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTgg                                                     <  1:1417030/109‑1 (MQ=255)
                                                                                                 tCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgttgtt                                             <  1:1560480/117‑1 (MQ=255)
                                                                                                 tCACTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgttgtt                                             >  2:1560480/1‑117 (MQ=255)
                                                                                                   aCTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTgg                                                     >  1:478800/1‑107 (MQ=255)
                                                                                                   aCTAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTgg                                                     <  2:478800/107‑1 (MQ=255)
                                                                                                     tAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgttgtt                                             >  1:88003/1‑113 (MQ=255)
                                                                                                     tAATGACTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgttgtt                                             <  2:88003/113‑1 (MQ=255)
                                                                                                          aCTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgttgtt                                             <  2:1355966/108‑1 (MQ=255)
                                                                                                          aCTTATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGgttgttgtt                                             >  1:1355966/1‑108 (MQ=255)
                                                                                                            ttATTTTCTGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGAttttt            >  1:574542/1‑139 (MQ=255)
                                                                                                                    tGCTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATaaa    >  1:345636/1‑139 (MQ=255)
                                                                                                                      cTTACCAAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATaaaaa  >  1:258306/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                      
TGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACC‑‑AAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAA  >  NC_000913/3922801‑3923056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: