| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 29.4% | 106.2 / 45.4 | 68 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (23/25); new base T (11/9); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCG > NC_000913/4295926‑4296195 | ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt > 1:545161/1‑139 (MQ=17)ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt < 1:670773/139‑1 (MQ=33) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACTTCAATGCCTg > 2:1728010/1‑139 (MQ=16) tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg > 1:720964/1‑139 (MQ=32) gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑g < 1:738174/139‑1 (MQ=32) tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGc > 1:1075660/1‑139 (MQ=32) aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGc < 1:210935/139‑1 (MQ=34) cAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCcgcg > 1:1224307/1‑139 (MQ=38) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGt < 1:1229434/139‑1 (MQ=38) cGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCctct > 2:1216733/1‑139 (MQ=255) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCt > 1:281038/1‑139 (MQ=25) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCt > 1:986342/1‑139 (MQ=25) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGt < 2:1247670/137‑1 (MQ=39) cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGt > 1:1247670/1‑137 (MQ=39) cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTAt < 2:281038/139‑1 (MQ=25) tGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAgg < 2:1050462/139‑1 (MQ=21) gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGc < 2:986342/139‑1 (MQ=21) gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGc < 2:1224307/139‑1 (MQ=38) gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGc < 1:212652/139‑1 (MQ=38) aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc > 2:557005/1‑106 (MQ=17) aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc < 1:557005/106‑1 (MQ=18) aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTa > 2:1877769/1‑139 (MQ=21) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATc > 1:1347158/1‑119 (MQ=39) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATc < 2:1347158/119‑1 (MQ=39) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGcc > 1:1147456/1‑114 (MQ=25) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCAACGCTTGCCGCGTCTTATCAGGcc < 2:1147456/114‑1 (MQ=255) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTAt < 1:1656244/105‑1 (MQ=255) cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTAt > 2:1656244/1‑105 (MQ=255) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCcgc < 1:1219123/97‑1 (MQ=40) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCcgc > 2:1219123/1‑97 (MQ=40) aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg < 2:1075660/139‑1 (MQ=38) gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGt > 1:1822059/1‑139 (MQ=37) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATc > 2:516703/1‑96 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATc < 1:516703/96‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGcc < 2:688502/101‑1 (MQ=25) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGcc > 1:688502/1‑101 (MQ=25) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGa > 2:856468/1‑139 (MQ=11) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCcgcg < 2:1658206/89‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCcgcg > 1:1658206/1‑89 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATc < 1:1164117/96‑1 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATc > 2:1164117/1‑96 (MQ=255) cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAttt > 1:618357/1‑139 (MQ=39) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt > 1:377274/1‑139 (MQ=37) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTAt > 2:529077/1‑89 (MQ=255) aGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTAt < 1:529077/89‑1 (MQ=255) tGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTACGCCGCATCCGACATCAACG‑CTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGa < 2:358259/139‑1 (MQ=255) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGcc < 2:469248/90‑1 (MQ=255) gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGcc > 1:469248/1‑90 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg < 1:561921/97‑1 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg > 2:561921/1‑97 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg < 2:1841959/113‑1 (MQ=39) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg > 1:1841959/1‑113 (MQ=40) aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa > 2:994283/1‑139 (MQ=39) tttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg < 2:618357/139‑1 (MQ=39) tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGACTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTATGCCGCATcc < 1:1216733/139‑1 (MQ=255) cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTTTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg < 1:1684131/139‑1 (MQ=255) cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg > 1:317174/1‑139 (MQ=38) cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCg < 2:1822059/139‑1 (MQ=38) ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGTCGCGTCTTATCAGGCCTACGCCAGACa > 2:625910/1‑78 (MQ=255) ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGTCGCGTCTTATCAGGCCTACGCCAGACa < 1:625910/78‑1 (MQ=255) gCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGGCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc < 2:377274/139‑1 (MQ=38) tttACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTTTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGcc > 2:1684131/1‑139 (MQ=255) tttACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa < 1:832760/74‑1 (MQ=255) tttACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa > 2:832760/1‑74 (MQ=255) cgcATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc < 2:1152777/114‑1 (MQ=38) cgcATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc > 1:1152777/1‑114 (MQ=37) cAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgcg > 1:22844/1‑139 (MQ=33) cAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgcg < 1:994283/139‑1 (MQ=33) | TTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAT‑‑‑GCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCG > NC_000913/4295926‑4296195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |