| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 548,249 | G→A | E524K (GAG→AAG) | fdrA → | putative NAD(P)‑binding acyl‑CoA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 548,249 | 0 | G | A | 100.0% | 251.3 / NA | 76 | E524K (GAG→AAG) | fdrA | putative NAD(P)‑binding acyl‑CoA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/40); total (36/40) | |||||||||||
GCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAA > NC_000913/548132‑548382 | gCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAAc < 1:1884015/139‑1 (MQ=255)gCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAAc < 1:1829720/139‑1 (MQ=255)gCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAAc < 2:1654339/139‑1 (MQ=255) aCCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCgg < 1:1838599/139‑1 (MQ=255) ccTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGt < 1:1313327/139‑1 (MQ=255) cTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAGAGCGCCAGCAAACCGGtt > 2:1262641/1‑139 (MQ=255) cTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCGTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGtt < 1:1799165/139‑1 (MQ=255) ttGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTtg < 1:559201/139‑1 (MQ=255) tGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTtgtg < 2:1547432/137‑1 (MQ=255) tGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTtgtg > 1:1547432/1‑137 (MQ=255) ttAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGt < 1:285328/139‑1 (MQ=255) tCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACAGGAAAACGTGGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACAAAGGGTCGCCAGTc > 1:1157858/1‑139 (MQ=255) gCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGcc > 1:625413/1‑139 (MQ=255) ctctCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGcc < 2:1345519/99‑1 (MQ=255) ctctCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGGTGTGCATTACCAATGGTTGCCAGttgtcg < 1:1092118/139‑6 (MQ=255) ctctCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTGTGCGCTGAAGCTACAAAGCGcc > 1:1345519/1‑99 (MQ=255) tctcGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCgg > 2:172687/1‑139 (MQ=255) cGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTgg < 1:1262641/139‑1 (MQ=255) tGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCaa < 1:627895/96‑1 (MQ=255) tGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCaa > 2:627895/1‑96 (MQ=255) aGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCa > 1:660913/1‑100 (MQ=255) aGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCa < 2:660913/100‑1 (MQ=255) cAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTgg > 1:1245200/1‑139 (MQ=255) aaCACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGc < 2:625413/139‑1 (MQ=255) cacacacCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAg > 2:789651/1‑112 (MQ=255) cacacacCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAg < 1:789651/112‑1 (MQ=255) acacacCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCg < 2:1096052/139‑1 (MQ=255) acCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGc < 2:142118/132‑1 (MQ=255) acCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGc > 1:142118/1‑132 (MQ=255) cATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAAc < 1:1347318/75‑1 (MQ=255) cATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAAc > 2:1347318/1‑75 (MQ=255) cATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGt < 2:93967/131‑1 (MQ=255) cATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGt > 1:93967/1‑131 (MQ=255) aTTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACAAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGaa > 2:1190056/1‑139 (MQ=255) tACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACg < 1:172687/139‑1 (MQ=255) cTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCa < 2:864801/64‑1 (MQ=255) cTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCa > 1:864801/1‑64 (MQ=255) ggAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTg > 2:1062512/1‑139 (MQ=255) gAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGc > 2:1666279/1‑139 (MQ=255) aCGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATa > 2:703229/1‑139 (MQ=255) cGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGtt > 2:1789749/1‑67 (MQ=255) cGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGtt < 1:1789749/67‑1 (MQ=255) cGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTg > 2:32911/1‑112 (MQ=255) cGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTg < 1:32911/112‑1 (MQ=255) tCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGc < 2:866519/42‑1 (MQ=255) tCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGc > 1:866519/1‑42 (MQ=255) tCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGc > 2:455116/1‑69 (MQ=255) tCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGc < 1:455116/69‑1 (MQ=255) ccGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCg < 1:644524/90‑1 (MQ=255) ccGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCg > 2:644524/1‑90 (MQ=255) ccGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATa > 1:503056/1‑99 (MQ=255) ccGTGATTAACATCGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATa < 2:503056/99‑1 (MQ=255) cGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGa < 2:1133966/108‑1 (MQ=255) cGGATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGa > 1:1133966/1‑108 (MQ=255) gATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGc < 2:949961/94‑1 (MQ=255) gATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGc > 1:949961/1‑94 (MQ=255) gATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCg > 1:503285/1‑97 (MQ=255) gATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCg < 2:503285/97‑1 (MQ=255) gATTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTCTACATc > 1:1366702/1‑139 (MQ=255) aTTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCa > 1:1778400/1‑51 (MQ=255) aTTACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCa < 2:1778400/51‑1 (MQ=255) ttACGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTCTACATCAg < 2:1366702/139‑1 (MQ=255) cGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTgg > 2:1222615/1‑139 (MQ=255) cGCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTgg > 1:1428527/1‑139 (MQ=255) gCAGCTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGgc < 2:709797/139‑1 (MQ=255) cTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGt > 2:1557785/1‑53 (MQ=255) cTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGt < 1:1557785/53‑1 (MQ=255) cTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGc > 2:1026027/1‑84 (MQ=255) cTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGc < 1:1026027/84‑1 (MQ=255) cTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATa < 1:1239498/108‑1 (MQ=255) cTTTGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATa > 2:1239498/1‑108 (MQ=255) ttGCGCTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGc < 1:703229/139‑1 (MQ=255) gcgcTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCa < 2:1720969/139‑1 (MQ=255) cgcTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGa > 1:1393546/1‑91 (MQ=255) cgcTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGa < 2:1393546/91‑1 (MQ=255) cgcTGAAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCaa > 1:1629784/1‑139 (MQ=255) | GCCACCTTGCTGGCGGCAGCGTTAATTCATCCGCTCTCGCCTGCCGCACAGCAACACACACCGTCATTACTGGAAAACGTCGCCGTGATTAACATCGGATTACGCAGCTTTGCGCTGGAGCTACAAAGCGCCAGCAAACCGGTTGTGCATTACCAATGGTCGCCAGTCGCCGGTGGCAATAAAAAACTGGCTCGTTTATTAGAACGTTTGCAATAAGGGGTTCCCATGTTTACATCAGTGGCGCAAGCCAA > NC_000913/548132‑548382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |