Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	58,436	C→T	intergenic (+257/‑38)	djlA → / → yabP	membrane‑anchored DnaK co‑chaperone, DNA‑binding protein/pseudogene, pentapeptide repeats‑containing

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	58,436	0	C	T	100.0%	192.3 / NA	59	intergenic (+257/‑38)	djlA/yabP	membrane‑anchored DnaK co‑chaperone, DNA‑binding protein/pseudogene, pentapeptide repeats‑containing
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

TTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGCGGGGACAAGATGGACGTGAAG  >  NC_000913/58302‑58569                                                                                                                                       |                                                                                                                                      ttCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGcat                                                                                                                                   >  2:1630263/1‑139 (MQ=255)    tGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGcatcat                                                                                                                                <  2:354783/139‑1 (MQ=255)                       aCCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCg                                                                                                             >  2:538758/1‑139 (MQ=255)                               tAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTAtt                                                                                                     <  1:538758/139‑1 (MQ=255)                                    ttGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCAt                                                                                                <  1:973522/139‑1 (MQ=255)                                       agagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGcatcat                                                                                                                                >  2:1461392/1‑104 (MQ=255)                                       agagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGcatcat                                                                                                                                <  1:648608/104‑1 (MQ=255)                                       agagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGcatcat                                                                                                                                <  1:1461392/104‑1 (MQ=255)                                       agagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGcatcat                                                                                                                                >  2:648608/1‑104 (MQ=255)                                       agagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGaa                                                                                             >  1:588815/1‑139 (MQ=255)                                        gagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGtttt                                                                                                                         <  2:850400/110‑1 (MQ=255)                                        gagCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGtttt                                                                                                                         >  1:850400/1‑110 (MQ=255)                                            tttACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTAt                                                                                        <  2:1454736/139‑1 (MQ=255)                                            tttACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTAt                                                                                        <  1:1618619/139‑1 (MQ=255)                                                   cgaGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCAt                                                                                                <  2:1606870/124‑1 (MQ=255)                                                   cgaGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCAt                                                                                                >  1:1606870/1‑124 (MQ=255)                                                     aGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAg                                                                               >  2:1770934/1‑139 (MQ=255)                                                          aaTTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATg                                                                          <  1:1770934/139‑1 (MQ=255)                                                                    ttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATg                                                                          >  1:1264899/1‑129 (MQ=255)                                                                    ttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATg                                                                          <  2:1264899/129‑1 (MQ=255)                                                                    ttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTacac                                                                >  2:558200/1‑139 (MQ=255)                                                                    ttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTac                                                                  <  2:1301899/137‑1 (MQ=255)                                                                    ttACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGACATGCCGGTTac                                                                  >  1:1301899/1‑137 (MQ=255)                                                                      aCGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACtt                                                              >  1:1664283/1‑139 (MQ=255)                                                                          cTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCt                                                                                                        <  1:1054163/93‑1 (MQ=255)                                                                          cTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCt                                                                                                        >  2:1054163/1‑93 (MQ=255)                                                                          cTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTac                                                                  <  1:1111126/131‑1 (MQ=255)                                                                          cTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTac                                                                  >  2:1111126/1‑131 (MQ=255)                                                                                         cacaTACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACg                                           <  1:558200/139‑1 (MQ=255)                                                                                            aTACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGa                                                                                              <  1:1453264/85‑1 (MQ=255)                                                                                            aTACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGa                                                                                              >  2:1453264/1‑85 (MQ=255)                                                                                             tACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGACATGCCGGTTACACTTTTAAATATGAGCAAGAACGatat                                       <  2:588815/139‑1 (MQ=255)                                                                                                   aaTGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGTCTATTACCATGAAAGTAt                                                                                        <  1:1044742/84‑1 (MQ=255)                                                                                                   aaTGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTAt                                                                                        >  2:1044742/1‑84 (MQ=255)                                                                                                   aaTGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTa                                                                   >  2:636817/1‑105 (MQ=255)                                                                                                   aaTGAGCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTa                                                                   <  1:636817/105‑1 (MQ=255)                                                                                                        gCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTAt                                                                                        <  2:1299654/79‑1 (MQ=255)                                                                                                        gCGATTTTTGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTAt                                                                                        >  1:1299654/1‑79 (MQ=255)                                                                                                            tttttGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAg                        <  1:1856099/139‑1 (MQ=255)                                                                                                             ttttGAAAGTATATTTATTCAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGc                       <  2:1664283/139‑1 (MQ=255)                                                                                                                            tattCAGAACGTGCATCATGAGTTTTTAACTCAATGc                                                                                                              <  1:269227/37‑1 (MQ=255)                                                                                                                            tattCAGAACGTGCATCATGAGTTTTTAACTCAATGc                                                                                                              >  2:269227/1‑37 (MQ=255)                                                                                                                                cAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTAt                                                                                        <  1:1584012/55‑1 (MQ=255)                                                                                                                                cAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTAt                                                                                        >  2:1584012/1‑55 (MQ=255)                                                                                                                                cAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTac                                                                  <  2:917512/77‑1 (MQ=255)                                                                                                                                cAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTac                                                                  >  1:917512/1‑77 (MQ=255)                                                                                                                                cAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAATGATATTTATAAGATGGTGAGCGGGGACAAGATGGACGTg     >  1:509707/1‑138 (MQ=255)                                                                                                                                cAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGCGGGGACAAGATGGACGTg     <  2:509707/138‑1 (MQ=255)                                                                                                                                cAGAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGCGGGGACAAGATGGACGTGa    >  1:460518/1‑139 (MQ=255)                                                                                                                                cAGAACGTACATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGCGGGGACAAGATGGACGTGa    >  1:1692439/1‑139 (MQ=255)                                                                                                                                  gAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGtt                                                                    <  2:1032049/73‑1 (MQ=255)                                                                                                                                  gAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGtt                                                                    >  1:1032049/1‑73 (MQ=255)                                                                                                                                  gAACGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGCGGGGACAAGATGGACGTGAAg  >  1:554051/1‑139 (MQ=255)                                                                                                                                   aaCGTGCATCATGAGTTTTTAACTCAATGCGAGGCTa                                                                                                       <  2:1209634/37‑1 (MQ=38)                                                                                                                                   aaCGTGCATCATGAGTTTTTAACTCAATGCGAGGCTa                                                                                                       >  1:1209634/1‑37 (MQ=255)                                                                                                                                   aaCGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACg                                           <  2:1520875/97‑1 (MQ=255)                                                                                                                                   aaCGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACg                                           >  1:1520875/1‑97 (MQ=255)                                                                                                                                   aaCGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGc                       <  2:350407/117‑1 (MQ=255)                                                                                                                                   aaCGTGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGc                       >  1:350407/1‑117 (MQ=255)                                                                                                                                       |                                                                                                                                      TTCTGTAGGTTTTATCGCGTTGACCCTGCTTAAGGTTGAGAGCTTTACGACGAGCGGAATTATATTTTTACGTCTTAAAAATAAAAAACACATACCTGAATGAGCGATTTTTGAAAGTATATTTATTCAGAACGCGCATCATGAGTTTTTAACTCAATGCGAGGCTATTACCATGAAAGTATCAGTTCCAGGCATGCCGGTTACACTTTTAAATATGAGCAAGAACGATATTTATAAGATGGTGAGCGGGGACAAGATGGACGTGAAG  >  NC_000913/58302‑58569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑