Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	672,947	C→A	V613F (GTT→TTT)	leuS ←	leucyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	672,947	0	C	A	98.4%	195.7 / NA	62	V613F (GTT→TTT)	leuS	leucyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (33/28);  minor base T (0/1);  total (33/29)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCA  >  NC_000913/672816‑673081                                                                                                                                    |                                                                                                                                       gagaGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCa                                                                                                                                 <  1:1685650/139‑1 (MQ=255)   cggTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTGCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATg                                                                                                                               <  2:1289283/137‑1 (MQ=255)     gTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGc                                                                                                                             <  2:444573/139‑1 (MQ=255)          atCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGcc                                                                                                                        <  1:333106/139‑1 (MQ=255)           tCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCg                                                                                                                       <  2:534611/139‑1 (MQ=255)              gCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCAt                                                                                                                    >  1:1850903/1‑139 (MQ=255)               ccGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATc                                                                                                                   >  2:1198102/1‑139 (MQ=255)                cGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCt                                                                                                                  >  1:398298/1‑139 (MQ=255)                cGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCt                                                                                                                  >  1:273904/1‑139 (MQ=255)                                atAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATAc                                                                                                  >  1:1532034/1‑139 (MQ=255)                                 tAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACg                                                                                                 >  1:1464055/1‑139 (MQ=255)                                 tAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACg                                                                                                 >  1:120547/1‑139 (MQ=255)                                     cAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCt                                                                                             >  2:210186/1‑139 (MQ=255)                                       gACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt                                                                                           >  1:391543/1‑139 (MQ=255)                                           aaCGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCg                                                                                       >  1:818105/1‑139 (MQ=255)                                             aggTGTCCGCGCTGTAACGTTCAACCACCACCGGCGGGTCGATACCGTTGTTCTTCGCCTTGGACATTTTGCTCATGACGGTATAAATCAGTTCATGGCCTGCCGTATCTTTCGCTTTCACGATACGGCCTTTCTCGTc                                                                                     <  1:1843159/138‑1 (MQ=255)                                                  tCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGtt                                                                                >  1:288308/1‑139 (MQ=255)                                                    cgcgCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCa                                                                                                        <  2:1777756/113‑1 (MQ=255)                                                    cgcgCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCa                                                                                                        >  1:1777756/1‑113 (MQ=255)                                                      cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGTTCATGCCGGTATAAAACAGTTCATGGCCTGCCg                                                                                                                       >  1:1833910/1‑96 (MQ=255)                                                      cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGTTCATGCCGGTATAAAACAGTTCATGGCCTGCCg                                                                                                                       <  2:1833910/96‑1 (MQ=255)                                                      cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCATGATACGGCCTTTCTCGTCACGTTCAAc                                                                            >  1:1317770/1‑139 (MQ=255)                                                      cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACg                                                                                                 <  2:1019845/118‑1 (MQ=255)                                                      cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACg                                                                                                 >  1:1019845/1‑118 (MQ=255)                                                      cgcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAAc                                                                            >  2:605436/1‑139 (MQ=255)                                                       gcCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACg                                                                           <  2:818105/139‑1 (MQ=255)                                                         cGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGAt                                                                         <  1:210186/139‑1 (MQ=255)                                                         cGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGAt                                                                         <  2:288308/139‑1 (MQ=255)                                                          gTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACCGTTCATGGCCTGCCGCATTTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAActgct                                                                        >  2:1843159/1‑135 (MQ=255)                                                            aaCGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGc                                                                      >  1:417801/1‑139 (MQ=255)                                                            aaCGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGc                                                                      >  2:1509417/1‑139 (MQ=255)                                                                  cAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                                <  1:822877/139‑1 (MQ=255)                                                                   aaCCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAcc                                                               >  2:1189716/1‑139 (MQ=255)                                                                     ccATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATGCGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCgg                                                             >  1:1182406/1‑139 (MQ=255)                                                                       aTCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCgggg                                                           <  2:391543/139‑1 (MQ=255)                                                                          aCCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGTCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaaa                                                        >  1:1529772/1‑139 (MQ=255)                                                                          aCCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaaa                                                        <  2:1494519/139‑1 (MQ=255)                                                                          aCCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaaa                                                        >  2:113749/1‑139 (MQ=255)                                                                             tGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAAccc                                                     >  2:784214/1‑139 (MQ=255)                                                                               cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                                <  2:1393777/126‑1 (MQ=255)                                                                               cGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTAACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                                >  1:1393777/1‑126 (MQ=255)                                                                                    cGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                                    >  2:150957/1‑101 (MQ=255)                                                                                    cGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCa                                                                                    <  1:150957/101‑1 (MQ=255)                                                                                      aTACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt                                                                                           <  2:185311/92‑1 (MQ=255)                                                                                      aTACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCttt                                                                                           >  1:185311/1‑92 (MQ=255)                                                                                      aTACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGtt                                            >  2:337791/1‑139 (MQ=255)                                                                                            ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaa                                                         <  2:367095/120‑1 (MQ=255)                                                                                            ttgttCTTCGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGaa                                                         >  1:367095/1‑120 (MQ=255)                                                                                                    cGACTTGGACATTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCa                              >  2:679354/1‑139 (MQ=255)                                                                                                              aTTTTGCTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCATGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGa                    <  2:1317770/139‑1 (MQ=255)                                                                                                                    cTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               <  1:1566720/138‑1 (MQ=255)                                                                                                                    cTCATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCa               >  2:1566720/1‑138 (MQ=255)                                                                                                                      cATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                                <  1:820882/87‑1 (MQ=255)                                                                                                                      cATGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAAc                                                                >  2:820882/1‑87 (MQ=255)                                                                                                                        tGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc          <  2:417801/139‑1 (MQ=255)                                                                                                                        tGCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGc          <  2:1532034/139‑1 (MQ=255)                                                                                                                         gCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTc                                                                                     >  1:731112/1‑63 (MQ=255)                                                                                                                         gCCGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTc                                                                                     <  2:731112/63‑1 (MQ=255)                                                                                                                          ccGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa        <  2:398298/139‑1 (MQ=255)                                                                                                                          ccGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa        <  2:273904/139‑1 (MQ=255)                                                                                                                          ccGGTATAAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCa        <  1:1189716/139‑1 (MQ=255)                                                                                                                                taAAACAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCa  <  2:120547/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       GAGAGTCATATCAGCCGGAGAAGCAAACATCATAAACAGACGAACGGTGTCCGCGCCGTAACGTTCAACCATCACCTGCGGGTCGATACCGTTGTTCTTCGACTTGGACATTTTGCTCATGCCGGTATAAACCAGTTCATGGCCTGCCGCATCTTTCGCTTTCACGATACGGCCTTTCTCGTCACGTTCAACGATAGCATCAACCGGGGAAACCCAGTTACGTTCGCCGTTTTCGCCAACATAGTAGAAGGCATCTGCCAGCACCA  >  NC_000913/672816‑673081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑