Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	681,418	C→T	R403C (CGT→TGT)	djlC →	J domain‑containing HscC co‑chaperone, Hsc56

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	681,418	0	C	T	100.0%	213.5 / NA	65	R403C (CGT→TGT)	djlC	J domain‑containing HscC co‑chaperone, Hsc56
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/34);  total (31/34)

TCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATG  >  NC_000913/681284‑681547                                                                                                                                       |                                                                                                                                  tcAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCt                                                                                                                               >  2:1265988/1‑139 (MQ=255)   aaGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTa                                                                                                                             >  2:1464028/1‑139 (MQ=255)    aGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTaa                                                                                                                            >  1:77283/1‑139 (MQ=255)      cgcAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATa                                                                                                                          <  2:77283/139‑1 (MQ=255)       gcAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATAc                                                                                                                         >  1:541942/1‑139 (MQ=255)          aaGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTAT‑GGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCt                                                                                                                               <  2:429302/129‑1 (MQ=255)          aaGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTAT‑GGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCt                                                                                                                               >  1:429302/1‑129 (MQ=255)                     tggtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCg                                                                                                           >  2:741355/1‑139 (MQ=255)                     tggtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCg                                                                                                           >  2:1825315/1‑139 (MQ=255)                     tggtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCg                                                                                                           >  2:368233/1‑139 (MQ=255)                       gtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGga                                                                                                         >  1:1509573/1‑139 (MQ=255)                       gtggAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGga                                                                                                         >  1:1250825/1‑139 (MQ=255)                          gAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGAt                                                                                                      <  1:1825315/139‑1 (MQ=255)                            aaTCGAGAGTCTGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                    <  1:912069/139‑1 (MQ=255)                            aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTg                                                                                                                  >  2:509641/1‑125 (MQ=255)                            aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTg                                                                                                                  <  1:509641/125‑1 (MQ=255)                            aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                    <  1:1464028/139‑1 (MQ=255)                            aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                    <  2:1509573/139‑1 (MQ=255)                            aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                    <  2:1250825/139‑1 (MQ=255)                            aaTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                    <  2:541942/139‑1 (MQ=255)                             aTCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCt                                                                                                   <  1:368233/139‑1 (MQ=255)                                gagaGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCAt                                                                                                <  2:466106/139‑1 (MQ=255)                                gagaGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCAt                                                                                                <  2:185622/139‑1 (MQ=255)                                  gagTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGc                                                                                                    <  2:1241873/133‑1 (MQ=255)                                  gagTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGAAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCCGAGATGc                                                                                                    >  1:1241873/1‑133 (MQ=255)                                       ggTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTag                                                                                         >  1:425014/1‑139 (MQ=255)                                          aGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAgg                                                                                      <  1:1265988/139‑1 (MQ=255)                                          aGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAgg                                                                                      <  1:741355/139‑1 (MQ=255)                                              agaCAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCAt                                                                                  <  2:425014/139‑1 (MQ=255)                                                         gATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGa                                                                       >  1:285270/1‑139 (MQ=255)                                                              aCCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCt                                                                                                                               <  1:910367/78‑1 (MQ=255)                                                              aCCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCt                                                                                                                               >  2:910367/1‑78 (MQ=255)                                                              aCCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTc                                                                                                  <  2:355277/107‑1 (MQ=255)                                                              aCCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTc                                                                                                  >  1:355277/1‑107 (MQ=255)                                                              aCCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCtt                                                                  >  1:504961/1‑139 (MQ=255)                                                                    ccTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCa                                                                                                 <  1:423679/102‑1 (MQ=255)                                                                    ccTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCa                                                                                                 >  2:423679/1‑102 (MQ=255)                                                                    ccTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACg                                                            <  2:651880/139‑1 (MQ=255)                                                                             gTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCAt                                                                                                <  1:1066296/94‑1 (MQ=255)                                                                             gTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCAt                                                                                                >  2:1066296/1‑94 (MQ=255)                                                                                      aGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCg                                          <  1:69487/139‑1 (MQ=255)                                                                                        cGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCgga                                        >  1:529597/1‑139 (MQ=255)                                                                                             cAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATg                                   <  1:99918/139‑1 (MQ=255)                                                                                                   gtaCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCg                                          <  2:554021/126‑1 (MQ=255)                                                                                                   gtaCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCg                                          >  1:554021/1‑126 (MQ=255)                                                                                                     aCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGc                                                                                     <  2:218550/81‑1 (MQ=255)                                                                                                     aCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGc                                                                                     >  1:218550/1‑81 (MQ=255)                                                                                                     aCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCg                                          >  2:51756/1‑124 (MQ=255)                                                                                                     aCTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCg                                          <  1:51756/124‑1 (MQ=255)                                                                                                     aCTTGAAGCGTTTCAGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTAACCCGGATGATTTTGAGGAAAGTGAAACTAGAGCAGGAGGATGAAGAGGTc                           >  1:120133/1‑139 (MQ=255)                                                                                                          aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTgg                                                                                                                 >  1:943167/1‑48 (MQ=255)                                                                                                          aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTgg                                                                                                                 <  2:943167/48‑1 (MQ=255)                                                                                                          aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGc                                                                                     >  2:1698453/1‑76 (MQ=255)                                                                                                          aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGc                                                                                     <  1:1698453/76‑1 (MQ=255)                                                                                                          aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGcc                                           <  2:585260/118‑1 (MQ=255)                                                                                                          aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGcc                                           >  1:585260/1‑118 (MQ=255)                                                                                                          aaGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGa                      >  2:58211/1‑139 (MQ=255)                                                                                                           aGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTAATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCggagg                                      >  1:861834/1‑122 (MQ=255)                                                                                                           aGCGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTAATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCggagg                                      <  2:861834/122‑1 (MQ=255)                                                                                                             cGTTTCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGGTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGcc                   <  2:285270/139‑1 (MQ=255)                                                                                                              atttCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCa                  >  1:1173424/2‑139 (MQ=255)                                                                                                               tttCCGAAACAAGAGATGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGTAGATGTCGATGAGCCaa                 <  2:1173424/139‑1 (MQ=255)                                                                                                                              aTGCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATATCGATGAGCCAACACCTGAAAATCATg  <  1:1120671/139‑1 (MQ=255)                                                                                                                                gCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAg                                <  2:128509/107‑1 (MQ=255)                                                                                                                                gCTGGCCTGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAg                                >  1:128509/1‑107 (MQ=255)                                                                                                                                       |                                                                                                                                  TCAAGCGCAAAGCATGGGCGTGGTGGAAATCGAGAGTCGGTAGCGAGACAGAGCAAGATTCACCGTTCCTGGATTTGTATCGGGTAGCGGAACAGGTAGTACTTGAAGCGTTTCCGAAACAAGAGATGCTGGCCCGTCTTAATACAAGGCTGGAAGGCGGAGATGCTCATCCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATG  >  NC_000913/681284‑681547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑