Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,545	C→T	intergenic (‑368/+29)	asnB ← / ← umpH	asparagine synthetase B/UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,545	0	C	T	100.0%	201.6 / NA	62	intergenic (‑368/+29)	asnB/umpH	asparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/30);  total (32/30)

AGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATC  >  NC_000913/699409‑699676                                                                                                                                         |                                                                                                                                    aGTGCGATTAGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCa                                                                                                                                   >  1:1018520/1‑139 (MQ=255)       aTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACg                                                                                                                             >  1:1401392/1‑139 (MQ=255)       aTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACg                                                                                                                             >  2:1230552/1‑139 (MQ=255)           gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCg                                                                                                                         <  2:775017/139‑1 (MQ=255)             tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCAc                                                                                                                              <  2:772035/132‑1 (MQ=255)             tCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCAc                                                                                                                              >  1:772035/1‑132 (MQ=255)                  ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGt                                                                                                                  <  2:909349/139‑1 (MQ=255)                      aTGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGtt                                                                                                              >  1:425132/1‑139 (MQ=255)                       tGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTg                                                                                                             <  1:607681/139‑1 (MQ=255)                           gcgTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTg                                                                                                             >  1:1116304/1‑135 (MQ=255)                           gcgTAAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAACCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTg                                                                                                             <  2:1116304/135‑1 (MQ=255)                             gTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTc                                                                                                       <  1:756239/139‑1 (MQ=255)                                             gCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTc                                                                                       >  2:402533/1‑139 (MQ=255)                                              ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTa                                                                                                          >  1:1295524/1‑119 (MQ=255)                                              ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTa                                                                                                          <  2:1295524/119‑1 (MQ=255)                                                    aaaGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACt                                                                                <  1:1123859/139‑1 (MQ=255)                                                          tGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACggg                                                                          <  2:1018520/139‑1 (MQ=255)                                                                          tGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGc                                                          >  2:1766078/1‑139 (MQ=255)                                                                               gAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAAcc                                                                                                                                <  2:467411/64‑1 (MQ=255)                                                                               gAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAAcc                                                                                                                                >  1:467411/1‑64 (MQ=255)                                                                               gAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                         >  1:27134/1‑135 (MQ=255)                                                                               gAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                         <  2:27134/135‑1 (MQ=255)                                                                               gAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                         <  2:1845879/135‑1 (MQ=255)                                                                               gAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                         >  1:1845879/1‑135 (MQ=255)                                                                                 gCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGt                                                                                                               >  2:160697/1‑79 (MQ=255)                                                                                 gCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGt                                                                                                               <  1:160697/79‑1 (MQ=255)                                                                                 gCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAgg                                                   >  2:320379/1‑139 (MQ=255)                                                                                  cTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGc                                                  >  2:1779136/1‑139 (MQ=255)                                                                                   tGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc                                                                                    <  2:991083/104‑1 (MQ=255)                                                                                   tGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc                                                                                    >  1:991083/1‑104 (MQ=255)                                                                                   tGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCa                                                 <  2:425132/139‑1 (MQ=255)                                                                                    gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCAc                                                                                                                              >  2:1852364/1‑61 (MQ=255)                                                                                    gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCAc                                                                                                                              <  1:1852364/61‑1 (MQ=255)                                                                                    gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGt                                                                                                                  <  1:1580227/73‑1 (MQ=255)                                                                                    gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGt                                                                                                                  >  2:1580227/1‑73 (MQ=255)                                                                                    gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGt                                                                                                               <  1:112063/76‑1 (MQ=255)                                                                                    gCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGt                                                                                                               >  2:112063/1‑76 (MQ=255)                                                                                     cGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATa                                               >  1:563560/1‑139 (MQ=255)                                                                                              gATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGata                                      <  1:402533/139‑1 (MQ=255)                                                                                               aTAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGatat                                     >  2:873455/1‑139 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTg                                                                                                             <  2:1002248/62‑1 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTg                                                                                                             >  1:1002248/1‑62 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATa                                                                                                  <  1:1340094/73‑1 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATa                                                                                                  >  2:1340094/1‑73 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                         <  1:1866818/114‑1 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCg                                                         >  2:1866818/1‑114 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGa                                                       <  2:1621078/116‑1 (MQ=255)                                                                                                    cGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGa                                                       >  1:1621078/1‑116 (MQ=255)                                                                                                      tAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGGGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAg                                                                                     >  1:843124/1‑84 (MQ=255)                                                                                                      tAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGGGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAg                                                                                     <  2:843124/84‑1 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCCACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcga                           >  1:279356/1‑139 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCa                                                                                                                               >  1:1741026/1‑39 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCa                                                                                                                               <  2:1741026/39‑1 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTg                                                                                                             >  1:893562/1‑57 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTg                                                                                                             <  2:893562/57‑1 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCCGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcga                           >  1:295073/1‑139 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcga                           <  2:1401392/139‑1 (MQ=255)                                                                                                         cgcATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcga                           >  1:1047204/1‑139 (MQ=255)                                                                                                           cATCAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGcgacg                         <  2:563560/139‑1 (MQ=255)                                                                                                              cAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                      >  2:303455/1‑139 (MQ=255)                                                                                                              cAGGCAGTTTGGCGTTTGTCATCAGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGaaa                      <  2:295073/139‑1 (MQ=255)                                                                                                                                  atcaGAGTCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACCGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATc  <  1:971367/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    AGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGGGTAAATCCAGCTGGGGCGGAAAGGCATACTGTCGATATCGTCGAGCGACGAAACACCAGAAAGCACCAGAATC  >  NC_000913/699409‑699676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑