Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	69,078	A→G	V324A (GTG→GCG)	araB ←	L‑ribulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	69,078	0	A	G	100.0%	207.6 / NA	58	V324A (GTG→GCG)	araB	L‑ribulokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/30);  total (28/30)

GCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCC  >  NC_000913/68943‑69202                                                                                                                                        |                                                                                                                             gcGTAGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGCTAAATCCAGGCACCgcgc                                                                                                                           <  2:1239403/139‑1 (MQ=255)  cGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCt                                                                                                                          >  1:863912/1‑139 (MQ=255)              ttttCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTg                                                                                                              <  2:863912/139‑1 (MQ=255)               tttCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGa                                                                                                             <  2:1506183/139‑1 (MQ=255)                     ttCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                       <  2:1441650/139‑1 (MQ=255)                             gCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCttt                                                                                               >  2:1374043/1‑139 (MQ=255)                               tGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTaa                                                                                             >  2:1397067/1‑139 (MQ=255)                                 ggcggcAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAAc                                                                                                                 <  1:1166688/117‑1 (MQ=255)                                 ggcggcAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAAc                                                                                                                 >  2:1166688/1‑117 (MQ=255)                                 ggcggcAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAAc                                                                                                                 >  2:1252165/1‑117 (MQ=255)                                 ggcggcAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAAc                                                                                                                 <  1:1252165/117‑1 (MQ=255)                                 ggcggcAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                       <  1:191878/127‑1 (MQ=255)                                 ggcggcAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                       >  2:191878/1‑127 (MQ=255)                                          cTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCg                                                                                  <  2:1349992/139‑1 (MQ=255)                                            gTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCAt                                                                                                                     <  1:752305/102‑1 (MQ=255)                                            gTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCAt                                                                                                                     >  2:752305/1‑102 (MQ=255)                                              tCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCaa                                                                              <  1:1374043/139‑1 (MQ=255)                                                  gCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGct                                                                          <  2:1477975/139‑1 (MQ=255)                                                            gAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCg                                                                <  1:1397067/139‑1 (MQ=255)                                                                    gACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                       <  2:1708213/92‑1 (MQ=255)                                                                    gACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                       >  1:1708213/1‑92 (MQ=255)                                                                    gACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAg                                                        >  1:810284/1‑139 (MQ=255)                                                                     aCCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGa                                                       <  1:321254/139‑1 (MQ=255)                                                                       cAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAAt                                                     >  2:388058/1‑139 (MQ=255)                                                                        aaaCCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATg                                                    <  1:1492179/139‑1 (MQ=255)                                                                                cGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGtt                                                                     >  1:1739982/1‑114 (MQ=255)                                                                                cGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGtt                                                                     <  2:1739982/114‑1 (MQ=255)                                                                                cGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGa                                                       <  2:864127/128‑1 (MQ=255)                                                                                cGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGa                                                       >  1:864127/1‑128 (MQ=255)                                                                                cGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGt                                            >  2:1798423/1‑139 (MQ=255)                                                                                    gATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGcc                                                                                >  2:592119/1‑99 (MQ=255)                                                                                    gATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGcc                                                                                <  1:592119/99‑1 (MQ=255)                                                                                    gATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACg                                                                            >  1:138924/1‑103 (MQ=255)                                                                                    gATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACg                                                                            <  2:138924/103‑1 (MQ=255)                                                                                         cacaAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTAcc                                   >  1:1211300/1‑139 (MQ=255)                                                                                         cACGAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGGCGCAGGTGGAAGTAcc                                   >  2:1279465/1‑139 (MQ=255)                                                                                         cACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGAc                                                                                                            <  1:1254040/66‑1 (MQ=255)                                                                                         cACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGAc                                                                                                            >  2:1254040/1‑66 (MQ=255)                                                                                         cACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                       >  1:142355/1‑71 (MQ=255)                                                                                         cACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCaa                                                                                                       <  2:142355/71‑1 (MQ=255)                                                                                         cACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTAcc                                   >  2:1059453/1‑139 (MQ=255)                                                                                               aCGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAAc                             >  1:1553589/1‑139 (MQ=255)                                                                                                     aTTGGCCTGCTTCCAGACCGATAAATCCAGGCACCgcgc                                                                                                                           <  1:744297/39‑1 (MQ=255)                                                                                                     aTTGGCCTGCTTCCAGACCGATAAATCCAGGCACCgcgc                                                                                                                           >  2:744297/1‑39 (MQ=255)                                                                                                     aTTGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTAc                       >  2:1684876/1‑139 (MQ=255)                                                                                                      ttGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCt                                                                                    <  1:1740473/77‑1 (MQ=255)                                                                                                      ttGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCt                                                                                    >  2:1740473/1‑77 (MQ=255)                                                                                                      ttGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTAcc                      >  1:73417/1‑139 (MQ=255)                                                                                                       tGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                     <  1:388058/139‑1 (MQ=255)                                                                                                       tGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                     <  2:810284/139‑1 (MQ=255)                                                                                                       tGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                     <  2:675198/139‑1 (MQ=255)                                                                                                       tGGCCTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                     <  1:1798423/139‑1 (MQ=255)                                                                                                       tGGACTGCTTCCAGACCGATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                     <  2:1211300/139‑1 (MQ=255)                                                                                                                        gATAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGtgcg    >  1:417455/1‑139 (MQ=255)                                                                                                                         aTAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCaa                                                                              >  1:140323/1‑64 (MQ=255)                                                                                                                         aTAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCaa                                                                              <  2:140323/64‑1 (MQ=255)                                                                                                                          tAAATCCAGGCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGtgcgcc  <  1:1279465/139‑1 (MQ=255)                                                                                                                                  ggCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                     >  1:649301/1‑112 (MQ=255)                                                                                                                                  ggCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCa                     <  2:649301/112‑1 (MQ=255)                                                                                                                                   gCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGct                                                                          <  1:1341468/58‑1 (MQ=255)                                                                                                                                   gCACCGCGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGct                                                                          >  2:1341468/1‑58 (MQ=255)                                                                                                                                        |                                                                                                                             GCGTTGATTTGCGTTTTCAGTTCCGGATGCTGGGCGGCAAGCTGTTCCAGCGGCCAGCCGAGTACGCGACCAAACCAGGCGTAGATATCACCAAACGCCGATTGGCCTGCTTCCAGACCGATAAATCCAGGCACCACGCTGCCATCAACCTGACCGCAAATACCTTTAACTGCCCGCTCGCCAACGCTCTGTTTGTCGGCAATCAGAATGTCGCAGGTGGAAGTACCGATAACTTTTACCAGTGCGTTAGGCTGTGCGCC  >  NC_000913/68943‑69202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑