Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	769,135	A→G	K386K (AAA→AAG)	mngB →	alpha‑mannosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	769,135	0	A	G	100.0%	221.3 / NA	63	K386K (AAA→AAG)	mngB	alpha‑mannosidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/31);  total (32/31)

TGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGT  >  NC_000913/769007‑769267                                                                                                                                 |                                                                                                                                     tgctgTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGt                                                                                                                            >  1:109744/1‑139 (MQ=255)       aGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGt                                                                                                                                 >  1:1023806/1‑128 (MQ=255)       aGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGt                                                                                                                                 <  2:1023806/128‑1 (MQ=255)           aCAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGAt                                                                                                                  >  2:1880134/1‑139 (MQ=255)           aCAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGAt                                                                                                                  >  2:523107/1‑139 (MQ=255)           aCAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGAt                                                                                                                  >  1:1410432/1‑139 (MQ=255)             aaaGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGc                                                                                                                >  1:66083/1‑139 (MQ=255)               aGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGccc                                                                                                              <  2:109744/139‑1 (MQ=255)                gTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGcc                                                                                                               <  2:1225915/137‑1 (MQ=255)                gTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGcc                                                                                                               >  1:1225915/1‑137 (MQ=255)                            aTCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTgaag                                                                                                 <  1:1496676/139‑1 (MQ=255)                            aTCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTgaag                                                                                                 <  1:1880134/139‑1 (MQ=255)                              cgtcgCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTgaagaa                                                                                               <  2:66083/139‑1 (MQ=255)                               gtcgCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGaagaag                                                                                              <  1:667424/139‑1 (MQ=255)                                tcgCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGt                                                                                             >  2:1087853/1‑139 (MQ=255)                                       cTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAac                                                                                      >  1:314203/1‑139 (MQ=255)                                         tCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAacac                                                                                    <  2:1410432/139‑1 (MQ=255)                                           gAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAAcacca                                                                                  <  2:67879/139‑1 (MQ=255)                                                      aGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTg                                                                       >  1:1846847/1‑139 (MQ=255)                                                           tGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTg                                                                                                                    >  1:1733256/1‑89 (MQ=255)                                                           tGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTg                                                                                                                    <  2:1733256/89‑1 (MQ=255)                                                           tGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTgcgcgc                                                                  >  1:642135/1‑139 (MQ=255)                                                           tGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTgcgcgc                                                                  >  1:569965/1‑139 (MQ=255)                                                            ggCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGaagaag                                                                                              >  1:79064/1‑110 (MQ=255)                                                            ggCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGaagaag                                                                                              <  2:79064/110‑1 (MQ=255)                                                                       tGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTaa                                                      >  1:1558842/1‑139 (MQ=255)                                                                       tGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTaa                                                      <  2:853919/139‑1 (MQ=255)                                                                              tttCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTg                                                                                                                    <  1:1659294/70‑1 (MQ=255)                                                                              tttCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTg                                                                                                                    >  2:1659294/1‑70 (MQ=255)                                                                               ttCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGtt                                                                                            <  2:1319807/93‑1 (MQ=255)                                                                               ttCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGtt                                                                                            >  1:1319807/1‑93 (MQ=255)                                                                                tCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTgaag                                                                                                 >  2:1173464/1‑87 (MQ=255)                                                                                tCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTgaag                                                                                                 <  1:1173464/87‑1 (MQ=255)                                                                                   aCATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGcc                                                                                                               <  1:932285/70‑1 (MQ=255)                                                                                   aCATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGcc                                                                                                               >  2:932285/1‑70 (MQ=255)                                                                                    cATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag                                                               <  1:634051/117‑1 (MQ=255)                                                                                    cATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag                                                               >  2:634051/1‑117 (MQ=255)                                                                                       gcgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTg                                                                                                                    <  1:1163266/61‑1 (MQ=255)                                                                                       gcgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTg                                                                                                                    >  2:1163266/1‑61 (MQ=255)                                                                                       gcgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag                                                               <  2:1155708/114‑1 (MQ=255)                                                                                       gcgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag                                                               >  1:1155708/1‑114 (MQ=255)                                                                                       gcgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATcgc                                      >  2:1542666/1‑139 (MQ=255)                                                                                        cgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAGGAAGTTATCAAcacca                                                                                  >  2:962925/1‑94 (MQ=255)                                                                                        cgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAGGAAGTTATCAAcacca                                                                                  <  1:962925/94‑1 (MQ=255)                                                                                        cgcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATcgcg                                     >  1:1499499/1‑139 (MQ=255)                                                                                        cgcAAAATTGCCGACAACATGCCACAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag                                                               <  2:1241746/113‑1 (MQ=255)                                                                                        cgcAAAATTGCCGACAACATGCCACAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCgccag                                                               >  1:1241746/1‑113 (MQ=255)                                                                                         gcAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCgg                                    <  1:1542666/139‑1 (MQ=255)                                                                                          cAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGt                                   <  1:1754433/139‑1 (MQ=255)                                                                                                    gACAACATGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTAc                         <  1:1087853/139‑1 (MQ=255)                                                                                                     acaacaTGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTAcc                        <  1:1287090/139‑1 (MQ=255)                                                                                                       aacaTGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCt                                                                                                                     <  2:1382256/44‑1 (MQ=255)                                                                                                       aacaTGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCt                                                                                                                     >  1:1382256/1‑44 (MQ=255)                                                                                                       aacaTGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTgcgcg                                                                   <  2:120987/94‑1 (MQ=255)                                                                                                       aacaTGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTgcgcg                                                                   >  1:120987/1‑94 (MQ=255)                                                                                                       aacaTGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTg                                                                       >  2:1230002/1‑90 (MQ=255)                                                                                                       aacaTGCCGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTg                                                                       <  1:1230002/90‑1 (MQ=255)                                                                                                             ccGCAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTa                <  1:1157246/139‑1 (MQ=255)                                                                                                                cAGAGCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTc             >  1:1858673/1‑139 (MQ=255)                                                                                                                 agagCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGc                               >  1:181352/1‑120 (MQ=255)                                                                                                                 agagCGACGCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGc                               <  2:181352/120‑1 (MQ=255)                                                                                                                         gCCGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGccc    >  2:774960/1‑139 (MQ=255)                                                                                                                           cGACAAGCTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGt  <  2:642135/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     TGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCTGAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCGCAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTTATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAGCCTGTACCGTATTTTATTCGCCATGCCCGT  >  NC_000913/769007‑769267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑