Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 72,229 C→T *233* (TAG→TAA thiQ ← thiamine/thiamine pyrophosphate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091372,2290CT100.0% 231.3 / NA 70*233* (TAG→TAAthiQthiamine/thiamine pyrophosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/33);  total (37/33)

GCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCA  >  NC_000913/72094‑72359
                                                                                                                                       |                                                                                                                                  
gCGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTaa                                                                                                                                 >  1:1459485/1‑139 (MQ=255)
 cGTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAAc                                                                                                                                >  1:1036244/1‑139 (MQ=255)
  gTAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAAcc                                                                                                                               >  2:1335384/1‑139 (MQ=255)
   tAAAGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAAccc                                                                                                                              >  1:233730/1‑139 (MQ=255)
      aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             <  2:344969/137‑1 (MQ=255)
      aGTGGTTGGGGTTTAAGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             >  1:344969/1‑137 (MQ=255)
                  ttaagagaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGt                                                                                                               >  1:1205531/5‑139 (MQ=255)
                    aagagaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGc                                                                                                             >  2:1452825/3‑139 (MQ=255)
                      gagaTAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCg                                                                                                           <  2:792728/139‑1 (MQ=255)
                          tAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGc                                                                                                       <  2:233730/139‑1 (MQ=255)
                            gTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCAc                                                                                                     <  2:1459485/139‑1 (MQ=255)
                                  tgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                               <  1:1296831/139‑1 (MQ=255)
                                  tgCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCt                                                                                               <  1:1335384/139‑1 (MQ=255)
                                    cGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTa                                                                                             >  2:1668584/1‑139 (MQ=255)
                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCg                                                                                          >  2:1442559/1‑139 (MQ=255)
                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCg                                                                                          <  1:1452825/139‑1 (MQ=255)
                                       gTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCg                                                                                          >  1:1614291/1‑139 (MQ=255)
                                              gATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTcaaca                                                                                   <  1:911738/139‑1 (MQ=255)
                                                    aCGCTTGCCACGTCTTATCAGGCCTACAAAACGCCCTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCAt                                                                             <  2:1205531/139‑1 (MQ=255)
                                                       cTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTgg                                                                          <  1:1442559/139‑1 (MQ=255)
                                                         tGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             <  2:298312/86‑1 (MQ=255)
                                                         tGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             >  1:298312/1‑86 (MQ=255)
                                                                 cctATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTAc                                                                                            <  2:445350/109‑1 (MQ=255)
                                                                 cctATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTAc                                                                                            >  1:445350/3‑111 (MQ=255)
                                                                 cTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTg                                                                >  2:1702110/1‑139 (MQ=255)
                                                                      cAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAgg                                                           <  2:1614291/139‑1 (MQ=255)
                                                                          ccTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             <  1:1514307/69‑1 (MQ=255)
                                                                          ccTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             >  2:1514307/1‑69 (MQ=255)
                                                                          ccTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGAt                                                       >  2:1231965/1‑139 (MQ=255)
                                                                           cTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCg                                                                                                           >  2:440803/1‑86 (MQ=255)
                                                                           cTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCg                                                                                                           <  1:440803/86‑1 (MQ=255)
                                                                            tACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATgc                                                     >  2:493637/1‑139 (MQ=255)
                                                                             aCAAAACGCACTACCCTTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                           <  1:1160319/68‑1 (MQ=255)
                                                                             aCAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                           >  2:1160319/1‑68 (MQ=255)
                                                                              cAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATgcgc                                                   <  1:493637/139‑1 (MQ=255)
                                                                              cAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATgcgc                                                   >  1:1146860/1‑139 (MQ=255)
                                                                              cAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGAGATgcgc                                                   <  1:1231965/139‑1 (MQ=255)
                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                           >  2:195874/1‑62 (MQ=255)
                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                           >  2:753257/1‑62 (MQ=255)
                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                           <  1:195874/62‑1 (MQ=255)
                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTa                                                                                                                           <  1:753257/62‑1 (MQ=255)
                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCg                                                >  2:793564/1‑137 (MQ=255)
                                                                                   cGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCg                                                <  1:793564/137‑1 (MQ=255)
                                                                                        tACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGa                                         >  1:22420/1‑139 (MQ=255)
                                                                                        tACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGa                                         <  2:1036244/139‑1 (MQ=255)
                                                                                             gTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGactact                                    >  2:1597291/1‑139 (MQ=255)
                                                                                              tAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGt                                                                         >  2:469556/1‑101 (MQ=255)
                                                                                              tAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGt                                                                         <  1:469556/101‑1 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATAc                                                                    >  2:1413844/1‑100 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATAc                                                                    <  1:1413844/100‑1 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGa                                         <  2:1802923/127‑1 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGa                                         >  1:1802923/1‑127 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                             >  1:164502/1‑139 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                             >  1:200660/1‑139 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAgc                             >  2:1246908/1‑139 (MQ=255)
                                                                                                    ggATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGAGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTTGGCGACTACTACCGAgc                             >  1:1242979/1‑139 (MQ=255)
                                                                                                     gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATc                                                                                                                        >  2:1071522/1‑47 (MQ=255)
                                                                                                     gATAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATc                                                                                                                        <  1:1071522/47‑1 (MQ=255)
                                                                                                       tAAGGCGTTCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGcgcg                          <  1:1246908/139‑1 (MQ=255)
                                                                                                            cGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             <  1:1479404/35‑1 (MQ=21)
                                                                                                            cGTTCACGCCGCATCCGACAGTGCATATTAACCCg                                                                                                                             >  2:1479404/1‑35 (MQ=255)
                                                                                                              ttCACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGAt                   >  2:1838552/1‑139 (MQ=255)
                                                                                                                cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                              <  1:1001018/78‑1 (MQ=255)
                                                                                                                cACGCCGCATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCa                                                                              >  2:1001018/1‑78 (MQ=255)
                                                                                                                  cgccgcATCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACGCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCcgc               <  1:1702110/139‑1 (MQ=255)
                                                                                                                        aTCCGACAGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCa         >  2:795672/1‑139 (MQ=255)
                                                                                                                               aGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCTACTACTACCGAGCGCTTGGCGATCCGCGCCGCTTCTTCCa  <  2:1146860/139‑1 (MQ=255)
                                                                                                                               aGTGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCa  <  1:1668584/139‑1 (MQ=255)
                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGt                                                                         <  2:729286/66‑1 (MQ=255)
                                                                                                                                 tGCATATTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGt                                                                         >  1:729286/1‑66 (MQ=255)
                                                                                                                                       |                                                                                                                                  
GCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCA  >  NC_000913/72094‑72359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: