| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 911,132 | A→G | intergenic (‑83/+50) | poxB ← / ← hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 911,132 | 0 | A | G | 98.3% | 210.3 / ‑6.8 | 60 | intergenic (‑83/+50) | poxB/hcr | pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding/HCP oxidoreductase, NADH‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (30/29); minor base C (0/1); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCA > NC_000913/910996‑911264 | cACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCt < 1:1062813/139‑1 (MQ=255) tGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCt < 1:42261/116‑1 (MQ=255) tGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCt > 2:42261/1‑116 (MQ=255) atatAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAat < 1:1681106/139‑1 (MQ=255) gCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAGATAGCCCGATGAAAGGAATATCATCggg < 2:1164189/139‑1 (MQ=255) tttgtttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCt > 2:1345180/1‑95 (MQ=255) tttCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGA‑AAGGGATGGGAGAGAAAGGAGGCGCt > 1:247734/1‑91 (MQ=255) ctcCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGAtaaggggaaaa > 2:1737823/1‑129 (MQ=255) ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa < 1:756401/112‑1 (MQ=255) ccTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa > 2:756401/1‑112 (MQ=255) ctaatgttaaTAAC‑GTAAAAAATTTAATTCCTTCGACCGA‑GGGGGAAAGGAATGGAAGA‑AAAAGAGGCCCCAACCGTTAAATTACCCCATTAAAGGAATTTCCACCGGCATAAAGCCATTATGCGAGAACCAAATcccc < 1:1737823/130‑1 (MQ=255) tGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATcccc > 1:677317/1‑139 (MQ=255) aaTGTGATAACGGTAACAAGTTTAGTTCATCTGACGGAGGGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCt < 1:1739497/70‑1 (MQ=255) aTGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTg > 1:71172/1‑139 (MQ=255) tgATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCgg < 1:409069/139‑1 (MQ=255) gATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCt < 2:1040696/64‑1 (MQ=255) aaCGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACg > 2:1440174/1‑65 (MQ=255) aaCGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACg < 1:1440174/65‑1 (MQ=255) gTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAgg > 1:1438346/1‑139 (MQ=255) aaCAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg > 2:590327/1‑139 (MQ=255) aaCAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAg < 2:71172/139‑1 (MQ=255) aCAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa < 1:293677/64‑1 (MQ=255) aCAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTaaa > 2:293677/1‑64 (MQ=255) aGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAgg > 1:790849/1‑139 (MQ=255) ttAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa < 1:642981/87‑1 (MQ=255) ttAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCa > 2:642981/1‑87 (MQ=255) ttCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat > 1:1841635/1‑77 (MQ=255) ttCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat < 2:1841635/77‑1 (MQ=255) cATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat < 1:22396/75‑1 (MQ=255) cATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat > 2:22396/1‑75 (MQ=255) tCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGc < 2:372260/54‑1 (MQ=255) tCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGc > 1:372260/1‑54 (MQ=255) cTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg > 1:597511/1‑56 (MQ=255) cTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCg < 2:597511/56‑1 (MQ=255) cTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa > 1:31279/1‑61 (MQ=255) cTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa < 2:31279/61‑1 (MQ=255) cGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTc > 2:436156/1‑139 (MQ=255) ggA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAAt < 1:547121/98‑1 (MQ=255) ggA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAAt > 2:547121/1‑98 (MQ=255) ggggg‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat < 1:585820/64‑1 (MQ=255) ggggg‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATatcat > 2:585820/1‑64 (MQ=255) gggg‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgag > 1:235149/1‑86 (MQ=255) gggg‑AAGGGATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgag < 2:235149/86‑1 (MQ=255) ggATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGc > 2:462013/1‑139 (MQ=255) gATGGGAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCg > 2:1302533/1‑139 (MQ=255) gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgaga < 2:1412210/76‑1 (MQ=255) gggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCgaga > 1:1412210/1‑76 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa < 2:260324/40‑1 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGaa > 1:260324/1‑40 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAata < 2:546545/47‑1 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAata > 1:546545/1‑47 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc > 2:345099/1‑97 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGc < 1:345099/97‑1 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCgg > 1:213212/1‑139 (MQ=255) ggAGAGAAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAG‑AGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGt < 1:927807/139‑1 (MQ=255) gagagaAAGGAGGCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGt < 2:790849/139‑1 (MQ=255) aggaggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa < 2:990664/101‑1 (MQ=255) aggaggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCa > 1:990664/1‑101 (MQ=255) ggaggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCa < 2:1438346/139‑1 (MQ=255) ggaggCGCTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCa < 1:462013/139‑1 (MQ=255) | CACCCCTGCCGATTCGAGTGTTTTGGCGATATAAGCTGCAACCGTTTGTTTCATGGTTCTCCATCTCCTGAATGTGATAACGGTAACAAGTTTAGTTCATCTGACGGA‑GGGGG‑AAGGGATGGGAGAGAAAGGAGGCACTAACGGTTAAATAGCCCGATGAAAGGAATATCATCGGGCATAAGGCGATTATGCGAGAACCAAATCCCCCTGCGGATGGCAGGAGCAGGCCAGTACGTAACCTTCAGCGATTTCGGCGTCGGTCAGCGTCA > NC_000913/910996‑911264 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |