Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,366,935 C→T *326* (TAG→TAA pspF ← psp operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,366,9350CT100.0% 294.9 / NA 89*326* (TAG→TAApspFpsp operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/43);  total (46/43)

TTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTG  >  NC_000913/1366812‑1367066
                                                                                                                           |                                                                                                                                   
ttAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCttt                                                                                                                      >  1:1580305/1‑139 (MQ=255)
        ccAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaacaa                                                                                                              >  1:1648455/1‑139 (MQ=255)
          aaCGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                            >  2:1641592/1‑139 (MQ=255)
          aaCGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                            >  1:462580/1‑139 (MQ=255)
            cGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          >  2:370889/1‑139 (MQ=255)
                 taatGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                     <  2:1580305/139‑1 (MQ=255)
                       cTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgatg                                                                                               <  1:1087356/139‑1 (MQ=255)
                       cTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgatg                                                                                               <  2:452721/139‑1 (MQ=255)
                         gATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                            <  1:461169/124‑1 (MQ=255)
                         gATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcg                                                                                                            >  2:461169/1‑124 (MQ=255)
                         gATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgatgaa                                                                                             >  1:1169316/1‑138 (MQ=255)
                           tgcgctgcgcTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          >  2:1342795/1‑124 (MQ=255)
                           tgcgctgcgcTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          <  1:1342795/124‑1 (MQ=255)
                           tgcgctgcgcTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATaa                                                                                           >  2:1735233/1‑139 (MQ=255)
                               ctgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAAt                                                                                                                                <  2:637673/98‑1 (MQ=255)
                               ctgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAAt                                                                                                                                >  1:637673/1‑98 (MQ=255)
                                tgcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTaa                                                                                      >  1:790531/1‑139 (MQ=255)
                                 gcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAAc                                                                                     <  1:1480976/139‑1 (MQ=255)
                                 gcgctTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAAc                                                                                     <  2:898289/139‑1 (MQ=255)
                                       aTCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTATCAACAACGCGCGGAAC‑‑‑TGATAAGTTAACCCCAGTAAt                                                                            >  2:1543735/1‑139 (MQ=255)
                                        tCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          <  2:289267/111‑1 (MQ=255)
                                        tCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          >  1:289267/1‑111 (MQ=255)
                                            gCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          <  2:1579438/107‑1 (MQ=255)
                                            gCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          >  1:1579438/1‑107 (MQ=255)
                                               tGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                     <  2:1008437/109‑1 (MQ=255)
                                               tGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                     >  1:1008437/1‑109 (MQ=255)
                                               tGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGc                                                                       >  2:1545920/1‑139 (MQ=255)
                                                gCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTATCAACAACGCGCGGAAC‑‑‑TGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                   <  1:1543735/139‑1 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaaca                                                                                                               >  2:1058491/1‑97 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTcaaca                                                                                                               <  1:1058491/97‑1 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGtt                                                                                        >  2:1445371/1‑120 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGtt                                                                                        <  1:1445371/120‑1 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAAc                                                                                     <  1:438555/123‑1 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAAc                                                                                     >  2:438555/1‑123 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcg                                                                     >  1:905274/1‑139 (MQ=255)
                                                 cAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcg                                                                     <  1:623762/139‑1 (MQ=255)
                                                   aaCGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                   <  1:370889/139‑1 (MQ=255)
                                                      gacgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAAtt                                                                           <  2:495008/128‑1 (MQ=255)
                                                      gacgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAAtt                                                                           >  1:495008/1‑128 (MQ=255)
                                                      gacgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCgcgcgc                                                                <  2:905274/139‑1 (MQ=255)
                                                       acgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCt                                                               <  2:1574607/139‑1 (MQ=255)
                                                       acgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCt                                                               <  2:1648455/139‑1 (MQ=255)
                                                       acgTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCt                                                               <  2:462580/139‑1 (MQ=255)
                                                           aTTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAg                                                                                <  2:433813/118‑1 (MQ=255)
                                                           aTTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAg                                                                                >  1:433813/1‑118 (MQ=255)
                                                                  atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAAc                                                                                                    <  2:979182/91‑1 (MQ=255)
                                                                  atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAAc                                                                                                    >  1:979182/1‑91 (MQ=255)
                                                                  atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          >  2:565309/1‑133 (MQ=255)
                                                                  atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          <  1:1579546/133‑1 (MQ=255)
                                                                  atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          >  2:1579546/1‑133 (MQ=255)
                                                                  atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          <  1:565309/133‑1 (MQ=255)
                                                                  atgtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTatat                                                    >  1:435141/1‑139 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                     >  1:1605682/1‑88 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGaa                                                                                                     <  2:1605682/88‑1 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgatga                                                                                              >  2:284462/1‑95 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtgatga                                                                                              <  1:284462/95‑1 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAg                                                                                          >  2:1335519/1‑99 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAg                                                                                          <  1:1335519/99‑1 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGt                                                         >  2:1555246/1‑132 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGt                                                         <  1:1555246/132‑1 (MQ=255)
                                                                    gtatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATAttt                                                  >  1:50557/1‑139 (MQ=255)
                                                                     tatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtg                                                                                                  >  1:503497/1‑90 (MQ=255)
                                                                     tatgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACtg                                                                                                  <  2:503497/90‑1 (MQ=255)
                                                                       tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAg                                                                                >  1:213547/1‑106 (MQ=255)
                                                                       tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAg                                                                                <  2:213547/106‑1 (MQ=255)
                                                                       tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          >  2:318594/1‑128 (MQ=255)
                                                                       tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          <  1:318594/128‑1 (MQ=255)
                                                                       tgCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTccc                                               >  2:1578413/1‑139 (MQ=255)
                                                                        gCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCt                                              >  2:1299141/1‑139 (MQ=255)
                                                                         ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          <  2:246336/78‑1 (MQ=255)
                                                                         ccGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAAcgcg                                                                                                          >  1:246336/1‑78 (MQ=255)
                                                                             aTAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCttgttg                                         >  1:1375087/1‑139 (MQ=255)
                                                                                      tCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAg                                >  2:157064/1‑139 (MQ=255)
                                                                                       cACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGt                               <  1:157064/139‑1 (MQ=255)
                                                                                             gcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagc                         <  2:435141/139‑1 (MQ=255)
                                                                                             gcATCCGGCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTgcagc                         <  1:880578/139‑1 (MQ=255)
                                                                                                   ggCAAGTTGTATTGCTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAActct                   <  1:1735233/139‑1 (MQ=255)
                                                                                                                 cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          <  1:270431/86‑1 (MQ=255)
                                                                                                                 cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTgg                                                          >  2:270431/1‑86 (MQ=255)
                                                                                                                 cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATAtt                                                   >  2:1449118/1‑93 (MQ=255)
                                                                                                                 cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATAtt                                                   <  1:1449118/93‑1 (MQ=255)
                                                                                                                 cTCAACTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCAt     >  2:324372/1‑139 (MQ=255)
                                                                                                                    aaCTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTg  >  1:817957/1‑139 (MQ=255)
                                                                                                                      catCGTTAAATCTGGTGCTTTGTCAACAACGCGCGGAACTgatgat                                                                                             >  1:1129745/3‑46 (MQ=38)
                                                                                                                      cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTgatgat                                                                                             <  2:1129745/46‑1 (MQ=255)
                                                                                                                      cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                   >  1:940344/1‑72 (MQ=255)
                                                                                                                      cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCcgcg                                                                   <  2:940344/72‑1 (MQ=255)
                                                                                                                      cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTg                                                           <  2:483210/80‑1 (MQ=255)
                                                                                                                      cTTCGTTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTg                                                           >  1:483210/1‑80 (MQ=255)
                                                                                                                           |                                                                                                                                   
TTAATAGCCCAACGCAATAATGTCTGATGCGCTGCGCTTATCAGGCCTGCAAACGACGTATTGATTATGTATGCCGAATAAAGCATTCACGCCGCATCCGGCAAGTTGTATTGCTCAACTTCGCTAAATCTGGTGCTTTTTCAACAACGCGCGGAACTGATGATAAGTTAACCCCAGTAATTCAGCCGCGCGCTTCTGGTTATATTTCCCTTGTTGCAAACTGAGTTGCAGCAACTCTTTTTCCTGCTGCATCTG  >  NC_000913/1366812‑1367066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: