Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	150,947	T→C	T3A (ACT→GCT)	yadC ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	150,947	0	T	C	100.0%	189.7 / NA	53	T3A (ACT→GCT)	yadC	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

AGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAAC  >  NC_000913/150812‑151056                                                                                                                                        |                                                                                                              aGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCtt                                                                                                            >  1:780817/1‑139 (MQ=255)  ggTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTc                                                                                                           >  1:1551720/1‑139 (MQ=255)      aTCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCtt                                                                                                            <  2:845247/134‑1 (MQ=255)      aTCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCtt                                                                                                            >  1:845247/1‑134 (MQ=255)                  gTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGt                                                                                           <  2:1551720/139‑1 (MQ=255)                     gAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                            <  2:694771/135‑1 (MQ=255)                     gAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATg                                                                                            >  1:694771/1‑135 (MQ=255)                     gAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTc                                                                                        >  2:1820397/1‑139 (MQ=255)                       ggggCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                      <  1:858961/139‑1 (MQ=255)                          gCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAGGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                      <  1:1328791/136‑1 (MQ=255)                          gCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                      >  2:1328791/1‑136 (MQ=255)                              cGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGtt                                                                               <  2:304194/139‑1 (MQ=255)                                    ttATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                              <  2:1508608/118‑1 (MQ=255)                                    ttATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCaa                                                                                              >  1:1508608/1‑118 (MQ=255)                                           cTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                      <  2:1498725/119‑1 (MQ=255)                                           cTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                      >  1:1498725/1‑119 (MQ=255)                                                gCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGttttt                                                             >  2:1605546/1‑139 (MQ=255)                                                           cacaATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTc                                                  >  1:350240/1‑139 (MQ=255)                                                            acaATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCg                                                 <  2:622458/139‑1 (MQ=255)                                                                tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTtttgtttg                                                        >  2:1374217/1‑128 (MQ=255)                                                                tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTtttgtttg                                                        <  1:1374217/128‑1 (MQ=255)                                                                tAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgtagt                                             >  2:1339028/1‑139 (MQ=255)                                                                  aaaCTTGTATATTCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTaa                                           <  1:1339028/139‑1 (MQ=255)                                                                     cTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                        <  1:1605546/139‑1 (MQ=255)                                                                     cTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAata                                        <  2:350240/139‑1 (MQ=255)                                                                               cACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAGTGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAaca                              >  1:1157624/1‑139 (MQ=255)                                                                                actgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                >  1:1769057/1‑120 (MQ=255)                                                                                actgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCgt                                                <  2:1769057/120‑1 (MQ=255)                                                                                 ctgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                            <  1:1820397/139‑1 (MQ=255)                                                                                  tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                           <  1:1593173/139‑1 (MQ=255)                                                                                  tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                           >  2:1593173/1‑139 (MQ=255)                                                                                  tgactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATATTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACAt                           <  2:1157624/139‑1 (MQ=255)                                                                                   gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                    <  2:771606/113‑1 (MQ=255)                                                                                   gactgTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                    >  1:771606/1‑113 (MQ=255)                                                                                        tATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                    <  2:694303/108‑1 (MQ=255)                                                                                        tATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTAt                                                    >  1:694303/1‑108 (MQ=255)                                                                                                caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                            <  2:866581/124‑1 (MQ=255)                                                                                                caaGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                            >  1:866581/1‑124 (MQ=255)                                                                                                  aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                      >  1:1879385/1‑64 (MQ=255)                                                                                                  aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCtt                                                                                      <  2:1879385/64‑1 (MQ=255)                                                                                                  aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                            >  1:323415/1‑122 (MQ=255)                                                                                                  aGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAAcaca                            <  2:323415/122‑1 (MQ=255)                                                                                                    aaTACAGTGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAg         <  2:780817/139‑1 (MQ=255)                                                                                                           tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGt                                                                                           >  1:61695/1‑50 (MQ=255)                                                                                                           tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGt                                                                                           <  2:61695/50‑1 (MQ=255)                                                                                                           tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc           >  2:943802/1‑130 (MQ=255)                                                                                                           tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc           <  1:943802/130‑1 (MQ=255)                                                                                                           tGCTAAAAAAAGAATGTACCTGAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAac  >  2:1371826/1‑139 (MQ=255)                                                                                                              tAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                      <  1:860048/36‑1 (MQ=255)                                                                                                              tAAAAAAAGAATGTACCTGAAGATAGCCTTCAtttt                                                                                                      >  2:860048/1‑36 (MQ=255)                                                                                                                                gAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAatat                                       >  1:1177123/1‑81 (MQ=255)                                                                                                                                gAAGATAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTCTTATTCGTAGTAAatat                                       <  2:1177123/81‑1 (MQ=255)                                                                                                                                     tAGCCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGAcc           <  1:840930/104‑1 (MQ=255)                                                                                                                                        |                                                                                                              AGGTCATCTGTGCGGCAGTGGAGGGGCCTCGATAGTTATCAACTCCTGCATTATTTCCCACAATAAAACTTGTATATGCACTGACTGTATTACAACAAGAATACAGTGCTAAAAAAAGAATGTACCTGAAGATAGTCTTCATTTTTACTCCAATGTTTCTTTATTGTTATGGTTTGCGCTGTTTTTGTTTGTTATTCGTAGTAAATATCCACTAACACATGGCTCATAAGTTGACCAGATGTAAC  >  NC_000913/150812‑151056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑