Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,467,921	C→T	*302* (TAG→TAA)	insD1 ←	IS2 transposase TnpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,467,921	0	C	T	100.0%	239.5 / NA	76	*302* (TAG→TAA)	insD1	IS2 transposase TnpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/40);  total (36/40)

AGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCT  >  NC_000913/1467785‑1468044                                                                                                                                          |                                                                                                                            agaATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTta                                                                                                                           <  2:1038086/139‑1 (MQ=255)   aaTTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTtata                                                                                                                         >  1:1572822/1‑139 (MQ=255)        ccTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTcc                                                                                                                    >  2:34257/1‑139 (MQ=255)             ggggTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACa                                                                                                               <  1:1013022/139‑1 (MQ=255)              gggTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                              <  1:1019568/139‑1 (MQ=255)              gggTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACAt                                                                                                              <  1:34257/139‑1 (MQ=255)                    gCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGGCATCTGTTa                                                                                                        >  1:1290303/1‑139 (MQ=255)                         ggTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                   <  2:1109269/139‑1 (MQ=255)                              ttCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                              <  1:1525116/139‑1 (MQ=255)                                         gATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt                                                                                                  <  2:670195/124‑1 (MQ=255)                                         gATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACtt                                                                                                  >  1:670195/1‑124 (MQ=255)                                         gATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                   >  1:1360246/1‑139 (MQ=255)                                         gATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGcc                                                                                   >  2:416446/1‑139 (MQ=255)                                           tAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 <  1:3022/139‑1 (MQ=255)                                            aaaGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTc                                                                                                                     >  1:14299/1‑102 (MQ=255)                                            aaaGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTc                                                                                                                     <  2:14299/102‑1 (MQ=255)                                                cTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGcc                                                                            <  1:653257/139‑1 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGCACCCTGGATTTGCCCTTATATTTCCAGa                                                                                                                 <  1:304552/99‑1 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTtat                                                                                                                          <  2:1053596/90‑1 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTtat                                                                                                                          >  1:1053596/1‑90 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGa                                                                                                                 >  2:304552/1‑99 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                              >  2:1854646/1‑118 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                              <  1:1854646/118‑1 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCa                                                                         >  1:8509/1‑139 (MQ=255)                                                   tGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCa                                                                         >  1:91248/1‑139 (MQ=255)                                                     cTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCa                                                                                                                   >  2:1462501/1‑95 (MQ=255)                                                     cTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCa                                                                                                                   <  1:1462501/95‑1 (MQ=255)                                                            ttGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTccc                                                                <  2:1630858/139‑1 (MQ=255)                                                             tGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                        <  1:512447/98‑1 (MQ=255)                                                             tGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTa                                                                                                        >  2:512447/1‑98 (MQ=255)                                                                  gTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          <  2:1290303/139‑1 (MQ=255)                                                                  gTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          <  2:91248/139‑1 (MQ=255)                                                                  gTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          <  2:8509/139‑1 (MQ=255)                                                                         tacgctaACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATa                                                   <  2:1326502/139‑1 (MQ=255)                                                                                aCAATGCAGGATATTGAACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAg                                             <  1:970568/139‑1 (MQ=255)                                                                                   aTGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCa                                         <  1:284566/139‑1 (MQ=255)                                                                                     gCAGGGTATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATAATCCCGTGGCGAGCGATAACCCAGCGCACt                                       <  2:1360246/139‑1 (MQ=255)                                                                                              tG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                              <  1:1611019/75‑1 (MQ=255)                                                                                              tG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAAcc                                                                                              >  2:1611019/1‑75 (MQ=255)                                                                                              tG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                           <  2:1881025/126‑1 (MQ=255)                                                                                              tG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                           >  1:1881025/1‑126 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACt                                                                                                   <  2:1332438/65‑1 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 >  2:425599/1‑83 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 <  1:425599/83‑1 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCAc                                        >  1:261762/1‑124 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCAc                                        <  2:261762/124‑1 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                         >  2:661563/1‑139 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCAtt                         >  2:1828750/1‑139 (MQ=255)                                                                                                    gTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATATCCAGACATCTGTTATCACt                                                                                                   >  1:1332438/1‑65 (MQ=255)                                                                                                     ttttGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTc                        <  1:661563/139‑1 (MQ=255)                                                                                                       ttGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          <  1:730837/103‑1 (MQ=255)                                                                                                       ttGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGgcg                                                          >  2:730837/1‑103 (MQ=255)                                                                                                       ttGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCg                                  >  2:695224/1‑127 (MQ=255)                                                                                                       ttGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCg                                  <  1:695224/127‑1 (MQ=255)                                                                                                        tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                           >  1:1330432/1‑117 (MQ=255)                                                                                                        tGAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                           <  2:1330432/117‑1 (MQ=255)                                                                                                         gAACATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTta                    <  1:416446/139‑1 (MQ=255)                                                                                                           aCATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 >  2:952348/1‑76 (MQ=255)                                                                                                           aCATCATGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCg                                                                                 <  1:952348/76‑1 (MQ=255)                                                                                                                atGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgc                                            <  1:552996/108‑1 (MQ=255)                                                                                                                atGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgc                                            >  2:552996/1‑108 (MQ=255)                                                                                                                 tGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgc                                            >  2:384759/1‑107 (MQ=255)                                                                                                                 tGGGACAAGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgc                                            <  1:384759/107‑1 (MQ=255)                                                                                                                       aaGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACg       >  1:1023843/1‑138 (MQ=25)                                                                                                                       aaGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACg       <  2:1023843/138‑1 (MQ=25)                                                                                                                       aaGAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGc      >  2:739658/1‑139 (MQ=25)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                            >  2:1353137/1‑83 (MQ=37)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTgg                                                            <  1:1353137/83‑1 (MQ=255)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                           >  2:528977/1‑100 (MQ=25)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                           <  1:528977/100‑1 (MQ=25)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct    >  2:677505/1‑139 (MQ=18)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct    >  1:460601/1‑139 (MQ=18)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct    >  2:875298/1‑139 (MQ=18)                                                                                                                         gAACCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct    >  2:1549441/1‑139 (MQ=18)                                                                                                                         gAACCATGGCTTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCct    >  1:406136/1‑139 (MQ=12)                                                                                                                           aCCCTGGATTTGCCCTTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCTTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCctct  <  2:460601/139‑1 (MQ=17)                                                                                                                                          |                                                                                                                            AGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTG‑ACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCT  >  NC_000913/1467785‑1468044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑