Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	152,036	C→T	V66I (GTA→ATA)	yadL ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	152,036	0	C	T	100.0%	178.8 / NA	55	V66I (GTA→ATA)	yadL	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/30);  total (25/30)

TCATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAA  >  NC_000913/151900‑152168                                                                                                                                         |                                                                                                                                     tCATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATatatatat                                                                                                                                    >  2:572147/1‑139 (MQ=255)  cATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATc                                                                                                                                   >  2:1181835/1‑139 (MQ=255)  cATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATc                                                                                                                                   >  2:113102/1‑139 (MQ=255)  cATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATc                                                                                                                                   >  2:826593/1‑139 (MQ=255)                     tgtgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                                <  1:904456/139‑1 (MQ=255)                               ttGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGAt                                                                                                      <  1:1188536/139‑1 (MQ=255)                                       ttttATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTc                                                                                                           <  2:1782581/126‑1 (MQ=255)                                       ttttATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTc                                                                                                           >  1:1782581/1‑126 (MQ=255)                                       ttttATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCa                                                                                              <  1:826593/139‑1 (MQ=255)                                           aTTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTa                                                                                          >  2:863932/1‑139 (MQ=255)                                                     ttttATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGc                                                                                <  1:113102/139‑1 (MQ=255)                                                             gggATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAg                                                                        >  1:857240/1‑139 (MQ=255)                                                               gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                      <  2:977243/139‑1 (MQ=255)                                                               gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                      >  1:818914/1‑139 (MQ=255)                                                               gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTa                                                                      <  1:863932/139‑1 (MQ=255)                                                                 tACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAcc                                                                    <  1:572147/139‑1 (MQ=255)                                                                       cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaacaa                                                                                                                     >  2:892682/1‑84 (MQ=255)                                                                       cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATcaacaa                                                                                                                     <  1:892682/84‑1 (MQ=255)                                                                       cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                                <  2:1600877/89‑1 (MQ=255)                                                                       cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                                >  1:1600877/1‑89 (MQ=255)                                                                       cacaGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTc                                                              <  1:1181835/139‑1 (MQ=255)                                                                          aGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTAc                                                           <  2:857240/139‑1 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                                <  2:477903/84‑1 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                                >  1:477903/1‑84 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGAt                                                                                                      >  2:726422/1‑94 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGAt                                                                                                      <  1:726422/94‑1 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCagag                                                                                                 >  1:1647012/1‑99 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCagag                                                                                                 <  2:1647012/99‑1 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCag                                                                                                   <  1:941361/97‑1 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCag                                                                                                   >  2:941361/1‑97 (MQ=255)                                                                            tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACag                                                         >  1:1067842/1‑139 (MQ=255)                                                                                      tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCAt                                                                 <  2:1740510/121‑1 (MQ=255)                                                                                      tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCAt                                                                 >  1:1740510/1‑121 (MQ=255)                                                                                      tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                            <  2:1851911/126‑1 (MQ=255)                                                                                      tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                            >  1:1851911/1‑126 (MQ=255)                                                                                      tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                               >  2:719170/1‑139 (MQ=255)                                                                                      tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                               >  1:1109028/1‑139 (MQ=255)                                                                                      tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTC‑ACAGTAGTAATAACg                                              >  1:996104/1‑139 (MQ=255)                                                                                        aGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTC‑ACAGTAGTAATAACGGt                                            <  2:996104/139‑1 (MQ=255)                                                                                                        cTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTc                                                                                                           >  2:1877654/1‑61 (MQ=255)                                                                                                        cTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTc                                                                                                           <  1:1877654/61‑1 (MQ=255)                                                                                                           tGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATActgtctgt                          <  2:818914/139‑1 (MQ=255)                                                                                                            ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                            >  2:1632380/1‑120 (MQ=255)                                                                                                            ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                            <  1:1632380/120‑1 (MQ=255)                                                                                                             gTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTg                        <  2:380301/139‑1 (MQ=255)                                                                                                             gTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTg                        <  2:1067842/139‑1 (MQ=255)                                                                                                                  ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                            >  1:858929/1‑114 (MQ=255)                                                                                                                  ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGt                                            <  2:858929/114‑1 (MQ=255)                                                                                                                    cATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATa                 <  2:1109028/139‑1 (MQ=255)                                                                                                                       tGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTAc                                                                           >  2:641668/1‑78 (MQ=255)                                                                                                                       tGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTAc                                                                           <  1:641668/78‑1 (MQ=255)                                                                                                                       tGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAaga              >  2:224816/1‑139 (MQ=255)                                                                                                                        gATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTc                                                              >  1:911079/1‑90 (MQ=255)                                                                                                                        gATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTc                                                              <  2:911079/90‑1 (MQ=255)                                                                                                                         aTTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagaga            <  1:719170/139‑1 (MQ=255)                                                                                                                         aTTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAagaga            <  1:224816/139‑1 (MQ=255)                                                                                                                                   tatatataTCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTGCAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGaa  >  2:176291/1‑139 (MQ=255)                                                                                                                                   tatatataTCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGaa  >  1:983603/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                     TCATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACGCTGAA  >  NC_000913/151900‑152168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑